ssmuБюллетень сибирской медициныBulletin of Siberian Medicine1682-03631819-3684Siberian State Medical University, the Ministry of Healthcare of the Russian Federation10.20538/1682-0363-2018-1-159-166ssmu-1126Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL PAPERSОсобенности специфической гиперчувствительности к пищевым антигенам молочного и злакового кластеров у детей с расстройством аутистического спектраFeatures of specific food hypersensitivity to dairy and cereal products in children with autism spectrum disorderЧеревкоН. А.CherevkoN. A.

Черевко Наталья Анатольевна, д-р мед. наук, доцент, кафедра иммунологии и аллергологии.

 

Cherevko Natalia A., DM, Associate Professor, Department of Immunology and Allergology.

 

chna@0370.ruСкирневскаяА. В.SkirnevskayaA. V.

Скирневская Александра Владимировна, врач, клинико-диагностическая лаборатория.

г. Томск, ул. Трифонова, 22Б.

Skirnevskaya Aleksandra V., Мedical  Laboratory Scientist.

22B, Trifonova, Tomsk, 634050.

РозенштейнМ. Ю.RosensteinM. Yu.

Розенштейн Марина Юзефовна,  PhD, врач диетолог-эндокринолог.

117393, г. Москва, ул. Архитектора Власова, 18.

Rosenstein Marina Yu., PhD,  Nutritionist-Endocrinologist.

18, Arkhitektora Vlasova Str., Moscow, 117393.

НовиковП. С.NovikovP. S.

Новиков Павел Сергеевич, ординатор.

634050, г. Томск, Московский тракт, 2 .

Novikov Pavel S., Medical Resident.

 

МуравейникО. А.MuraveinikO. A.

Муравейник Ольга Анатольевна, врач, клинико-диагностическая лаборатория.

 

Muraveinik Olga A., Medical  Laboratory Scientist.

 

ДенисовА. А.DenisovA. A.

Денисов Андрей Алексадрович, д-р мед. наук, профессор, кафедра иммунологии и аллергологии.

634050, г. Томск, Московский тракт, 2 .

Denisov Andrei A., DM, Рrofessor, Department of Immunology and Allergology.

2, Moskow Tract, Tomsk, 634055.

Сибирский государственный медицинский университет (СибГМУ).РоссияSiberian State Medical University (SSMU) .Russian FederationМедицинское объединение «Центр семейной медицины» (МО ЦСМ).РоссияFamily Medicine Center .Russian FederationImmunoHealth РУС .РоссияImmunoHealth RUS .Russian Federation201830032018171159166Copyright © Черевко Н.А., Скирневская А.В., Розенштейн М.Ю., Новиков П.С., Муравейник О.А., Денисов А.А., 20182018Черевко Н.А., Скирневская А.В., Розенштейн М.Ю., Новиков П.С., Муравейник О.А., Денисов А.А.Cherevko N.A., Skirnevskaya A.V., Rosenstein M.Y., Novikov P.S., Muraveinik O.A., Denisov A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://bulletin.ssmu.ru/jour/article/view/1126

Целью работы являлось исследование взаимосвязи между специфической гиперчувствительностью к пищевым антигенам (пАГ), параметрами воспаления и цитокинового профиля у детей с расстройствами аутистического спектра (РАС).

Материалы и методы

Материалы и методы. В исследовании использовались иммунологические методы определения концентрации специфических иммуноглобулинов к 111 пищевым антигенам в модифицированном методе иммуно-ферментного анализа с применением методологии компании «Иммунохелс», определялась концентрация интерлейкинов (ИЛ) и биохимических показателей в сыворотке крови. Для оценки изменения физического и психического состояния детей с РАС до и после соблюдения рекомендованной элиминационной диеты с исключением пАГ в установленные сроки 0–6 мес родители заполняли специализированную анкету Bernard Rimland and Stephen M. Edelson of the Autism Research Institute (АТЕС).

Результаты

Результаты. Выявлено, что у детей с РАС статистически значимо чаще встречается гиперчувствительность к пАГ злаковых, молочных продуктов по сравнению с детьми из группы контроля. Степень гиперчувствительности к пАГ связана с результатами АТЕС, оцениваемыми в баллах. Обнаружены различия в показателях цитокинового профиля в исследуемых группах. У детей с РАС повышены значения интерферона (ИФН) γ в сыворотке крови, а также коэффициенты соотношений ИФНγ/ИЛ-10 и ИФНγ/ИЛ-4 по сравнению с этими показателями у детей контрольной группы. Выявленные изменения цитокинового профиля коррелируют с показателями гиперчувствительности к пАГ молочного и злакового кластеров и специализированного теста АТЕС.

The aim of the study was to investigate the relationship between food hypersensitivity, inflammation markers and the cytokine profile in children with autism spectrum disorders (ASD).

Materials and methods. The concentration of specific IgG for 111 food antigens was measured via the modified immunological method designed by the ImmunoHealth company. In addition, we performed routine biochemistry tests and immunoassay of interleukins. All children with ASD followed the recommended eliminating diet during 6 months. In order to check their physical and mental condition, their parents completed the autism treatment evaluation checklist (ATEC), both before and after the diet (Bernard Rimland and Stephen M. Edelson of the Autism Research Institute).

In result, we detected the high frequency of hypersensitivity to cereal and dairy products in children with ASD. Moreover, the intensity of hypersensitivity correlated with ATEC score. We also determined that the cytokine profile in children with ASD was different from that in the control group. In particular, the concentration of IFNγ in serum, as well as the IFNγ/IL-10 and IFNγ/IL-4 ratio, were significantly higher in children with ASD. The revealed changes in the cytokine profile correlate with the specific hypersensitivity for cereal and dairy food antigens as well as with ATEC score.

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The authors declare that there are no conflicts of interest present.