ssmuБюллетень сибирской медициныBulletin of Siberian Medicine1682-03631819-3684Siberian State Medical University, the Ministry of Healthcare of the Russian Federation10.20538/1682-0363-2018-3-80-87ssmu-1286Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL PAPERSОсобенности экспрессии CCL11/эотаксина, рецептора CCR3 и эозинофильной пероксидазы в опухолевой ткани при раке желудка и толстого кишечникаThe expression of CCL11/eotaxin, CCR3 receptor and eosinophil peroxidase in tumor tissue in gastric and colon cancershttps://orcid.org/0000-0001-7156-2471КолобовниковаЮ. В.KolobovnikovaYu. V.

Колобовникова Юлия Владимировна - докторр медицинских наук, профессор, кафедра патофизиологии.

634050, Томск, Московский тракт, 2; 634050, Томск, пр. Ленина, 115

Kolobovnikova Yuliya V. - DM, Professor, Department of Pathophysiology.

2, Moskow Trakt, Tomsk, 634050

kolobovnikova.julia@mail.ruhttps://orcid.org/0000-0001-8893-0939ЯнковичК. И.YankovichK. I.

Янкович Кристина Игоревна - младший научный сотрудник, ЦНИЛ, СибГМУ; врач клинико-диагностической лаборатории.

634050, Томск, Московский тракт, 2

Jankovich Kristina I. - Junior Researcher, Central Research Laboratory, SSMU; Doctor, Clinical and Diagnostic Laboratory, TROD.

2, Moskow Trakt, Tomsk, 634050; 115, Lenin S., Tomsk, 634050

https://orcid.org/0000-0002-8769-5743РомановаЕ. В.RomanovaE. V.

Романова Елена Викторовна - старший преподаватель, кафедра микробиологии и вирусологии.

634050, Томск, Московский тракт, 2

Romanova Elena V. - Senior Lecturer, Department of Microbiology and Virology.

2, Moskow Trakt, Tomsk, 634050

https://orcid.org/0000-0002-5247-9872ДмитриеваА. И.DmitrievaA. I.

Дмитриева Алла Ивановна - докторр медицинских наук, зав. клинико-диагностической лабораторией.

634050, Томск, пр. Ленина, 115

Dmitrieva Alla I. - DM, Head of the Clinical and Diagnostic Laboratory.

115, Lenin S., Tomsk, 634050

https://orcid.org/0000-0002-9577-8370НовицкийВ. В.NovitskiyV. V.

Новицкий Вячеслав Викторович - докторр медицинских наук, профессор, академик РАН, заслуженный деятель науки Российской Федерации, кафедра патофизиологии.

634050, Томск, Московский тракт, 2

Novitskiy Vyacheslav V. - DM, Professor, Academician of RAS, Honored Scientist of the Russian Federation, Department of Pathophysiology.

2, Moskow Trakt, Tomsk, 634050

https://orcid.org/0000-0002-9457-8879УразоваО. И.UrazovaO. I.

Уразова Ольга Ивановна - докторр медицинских наук, профессор, член-корреспондент РАН, заведующая кафедрой патофизиологии.

634050, Томск, Московский тракт, 2

Urazova Olga I. - DM, Professor, Corresponding Member of RAS, Head of the Department of Pathophysiology.

2, Moskow Trakt, Tomsk, 634050

Сибирский государственный медицинский университет (СибГМУ)РоссияSiberian State Medical University (SSMU)Russian FederationСибирский государственный медицинский университет (СибГМУ); Томский областной онкологический диспансер (ТООД)РоссияSiberian State Medical University (SSMU); Tomsk Regional Oncologic Dispensary (TROD)Russian FederationТомский областной онкологический диспансер (ТООД)РоссияTomsk Regional Oncologic Dispensary (TROD)Russian Federation2018290920181738087Copyright © Колобовникова Ю.В., Янкович К.И., Романова Е.В., Дмитриева А.И., Новицкий В.В., Уразова О.И., 20182018Колобовникова Ю.В., Янкович К.И., Романова Е.В., Дмитриева А.И., Новицкий В.В., Уразова О.И.Kolobovnikova Y.V., Yankovich K.I., Romanova E.V., Dmitrieva A.I., Novitskiy V.V., Urazova O.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://bulletin.ssmu.ru/jour/article/view/1286

Цель исследования – проанализировать экспрессию CCL11/эотаксина, рецептора к эотаксину CCR3 и эозинофильной пероксидазы (ЕРХ) в опухолевой ткани и ее связь с тканевой эозинофилией при раке желудка и толстого кишечника.

Материалы и методы

Материалы и методы. Обследовано 52 пациента с раком желудка и 55 пациентов с раком толстого кишечника. Материалом исследования служили образцы злокачественных новообразований желудка и толстого кишечника, полученные при операционном вмешательстве. Экспрессию CCL11/эотаксина, CCR3 и ЕРХ в опухолевой ткани оценивали иммуногистохимическим методом. Для статистической обработки результатов применяли  однофакторный дисперсионный и корреляционно-регрессионный (по Спирмену) методы анализа.

Результаты

Результаты. Для рака желудка и толстого кишечника с эозинофильной инфильтрацией опухолевой ткани характерна высокая экспрессия CCL11/эотаксина опухолевыми клетками. Рецептор CCR3 к эотаксину на мембране клеток инфильтрата опухолевой ткани при раке желудка и толстого кишечника обнаруживается в 100% случаев. Экспрессия CCR3 (при раке желудка) и ЕРХ (при раке желудка и толстого кишечника) клетками микроокружения злокачеcтвенных новообразований с инфильтрацией эозинофильными гранулоцитами существенно выше, чем в клетках микроокружения опухолей без эозинофильной инфильтрации. =

The purpose of the study was to analyze the expression of CCL11/eotaxin, CCR3 receptor to eotaxin and eosinophil peroxidase (EXP) in tumor tissue and its relation to tissue eosinophilia in gastric and colon cancers.

Materials and methods

Materials and methods. 52 patients with gastric cancer and 55 patients with colon cancer were examined. The material of the study was samples of malignant tumors of the stomach and large intestine obtained during a surgery. The expression of CCL11/eotaxin, CCR3 and EXP in the tumor tissue was evaluated by immunohistochemical method. For statistical processing of the results, one-factor dispersion and correlationregression (by Spearman) methods of analysis were used.

Results

Results. High expression of CCL11/eotaxin by tumor cells is typical of stomach and colon cancers with eosinophilic infiltration of the tumor tissue. The CCR3 receptor to eotaxin on the cell membrane of infiltration of the tumor tissue in gastric and colon cancers is found in 100% of cases. Expression of CCR3 (at stomach cancer) and EXP (at stomach and colon cancers) by cells of the tumor microenvironment with eosinophilic granulocytes infiltration is significantly higher than in the cells of the tumor microenvironment without eosinophilic infiltration.

Conclusion

Conclusion. Tissue eosinophilia in gastric and colon cancers develops due to the ability of transformed malignant cells to produce CCL11/eotaxin that mediates the attraction of CCR3-expressing eosinophil granulocytes in the tumor tissue. High level of EPX (marker enzyme of eosinophils) expression by cells of tumor microenvironment in the gastric and colon cancers with tissue eosinophilia suggests the expressed cytotoxic potential of eosinophilic granulocytes, which can be directed against tumor cells.

эозинофилияэотаксинCCR3эозинофильная пероксидазарак желудкарак толстого кишечникаeosinophiliaeotaxinCCR3eosinophilic peroxidasegastric cancercolon cancerСовет по грантам Президента Российской Федерации для государственной поддержки молодых российских ученых (МД-842.2017.7 «Роль галектинов в патогенезе рака желудка и толстой кишки с опухолеассоциированной эозинофилией», руководитель – докторр медицинских наук Ю.В. Колобовникова) и ведущих научных школ (НШ-2690.2018.7 «Молекулярные факторы дизрегуляции гомеостаза иммунокомпетентных клеток крови при социально-значимых заболеваниях» (руководитель – докторр медицинских наук, профессор, член-корреспондент РАН О.И. Уразова)Russian Federation President Council on Grants for governmental support for young Russian scientists (МД842.2017.7 “The role of galectins in the pathogenesis of stomach and colon cancer with tumor-associated eosinophilia”, supervisor – DmedSc Yu. V. 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The authors declare that there are no conflicts of interest present.