ssmuБюллетень сибирской медициныBulletin of Siberian Medicine1682-03631819-3684Siberian State Medical University, the Ministry of Healthcare of the Russian Federation10.20538/1682-0363-2018-3-188-196ssmu-1298Research ArticleОБЗОРЫ И ЛЕКЦИИREVIEW AND LECTURESДвухмерные и трехмерные модели культур клеток опухолей in vitro: преимущества и недостаткиTwo-dimensional and three-dimensional cell culture models in vitro: pros and consГалимоваЭ. С.GalimovaE. S.

Галимова Эльвира Сафуановна - кандидат биологических наук, старший научный сотрудник, отдел биохимических исследований, Центр доклинических и трансляционных исследований, Институт ýкспериментальной медицины.

197341, Санкт-Петербург, ул. Аккуратова, 2

Galimova Elvira S. -  PhD, Senior Researcher.

2, Accuratov Str., Saint Petersburg, 197341

elya-4@yandex.ruГалагудзаМ. М.GalagudzaM. М.

Галагудза Михаил Михайлович - докторр медицинских наук, член-корр. РАН, директор Института ýкспериментальной медицины.

197341, Санкт-Петербург, ул. Аккуратова, 2

Galagudza  Michael M. - DM., Professor, Head of Institute of Experimental Medicine.

2, Accuratov Str., Saint Petersburg, 197341

Национальный медицинский исследовательский центр (НМИЦ) им. В.А. АлмазоваРоссияAlmazov National Medical Research CenterRussian Federation201829092018173188196Copyright © Галимова Э.С., Галагудза М.М., 20182018Галимова Э.С., Галагудза М.М.Galimova E.S., Galagudza M.М.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://bulletin.ssmu.ru/jour/article/view/1298

Синтез и внедрение новых химических соединений, обладающих потенциальной противоопухолевой активностью, требуют надежных предиктивных доклинических моделей для скрининга эффективности in vitro. Такие модели включают культуры клеток опухолей человека – двухмерные системы культур клеток 2D (two-dimensional cell culture systems) и трехмерные системы культур клеток 3D (three-dimensional cell culture systems). В этом обзоре обсуждаются особенности молекулярного фенотипа клеток, культивируемых в 2Dand 3D-системах, и их применение в исследованиях эффективности противоопухолевых препаратов с упором на ключевые примеры из научной литературы. В обзоре также проанализированы преимущества, недостатки и перспективы применения описываемых моделей культур опухолевых клеток.

Discovery and development of new chemical compounds with putative anti-cancer properties requires reliable predictive preclinical models for in vitro screening of efficacy. Such models mainly include cultures of human cancer cells: two-dimensional (2D) and three-dimensional (3D) cell culture systems. In this review, we discuss the molecular aspects of cells cultured in 2D and 3D, and their relevance to cancer study, focusing on key examples from the recent literature. Advantages, disadvantages and perspectives of described models are also analyzed.

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The authors declare that there are no conflicts of interest present.