References

ssmu

Бюллетень сибирской медицины

Bulletin of Siberian Medicine

1682-03631819-3684

Siberian State Medical University, the Ministry of Healthcare of the Russian Federation

10.20538/1682-0363-2010-4-5-18

ssmu-1617

Research Article

ЭКСПЕРИМЕНТАЛЬНЫЕ И КЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯ

EXPERIMENTAL AND CLINICAL INVESTIGATIONS

Клещевой энцефалит: иммунологические показатели возможного перехода острой стадии в хроническое течение болезни

Tick-borne encephalitis: immunological indicators of possible transformation of acute process into chronic disease

Гусев

Е. И.

Gusev

Ye. I.

д-р мед. наук, профессор, академик РАМН, зав. кафедрой неврологии и нейрохирургии

Пархоменко

Т. А.

Parkhomenko

T. A.

аспирант

Бунева

В. Н.

Buneva

V. N.

д-р мед. наук, профессор, главный научный сотрудник

Доронина

О. Б.

Doronina

O. B.

канд. мед. наук, доцент кафедры неврологии

Доронин

В. Б.

Doronin

V. B.

клинический ординатор

Генералов

И. И.

Generalov

I. I.

д-р мед. наук, профессор, научный сотрудник

Доронин

Б. М.

Doronin

B. M.

д-р мед. наук, профессор, зав. кафедрой неврологии

Невинский

Г. А.

Nevinsky

G. A.

Невинский Георгий Александрович, д-р хим. наук, профессор, зав. лабораторией ферментов репарации

8 (383) 363-51-26, факс: 8 (383) 333-36-77

nevinsky@niboch.nsc.ru

Российский государственный медицинский университетRussian FederationИнститут химической биологии и фундаментальной медицины СО РАН; Новосибирский государственный университетRussian FederationНовосибирский государственный медицинский университетRussian FederationИнститут терапии СО РАМНRussian FederationВитебский государственный медицинский университетRussian Federation

2010

28082010

94518

2010

Гусев Е.И., Пархоменко Т.А., Бунева В.Н., Доронина О.Б., Доронин В.Б., Генералов И.И., Доронин Б.М., Невинский Г.А.

Gusev Y.I., Parkhomenko T.A., Buneva V.N., Doronina O.B., Doronin V.B., Generalov I.I., Doronin B.M., Nevinsky G.A.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://bulletin.ssmu.ru/jour/article/view/1617

Ряд аутоиммунных заболеваний с хроническим течением характеризуются обнаружением в крови больных ДНКаутоантител, в то время как их не содержит кровь здоровых доноров или пациентов с острым течением заболеваний, незначительным нарушением иммунного статуса, без определенной склонности к переходу в хронический процесс. Клещевой энцефалит (КЭ) не рассматривался с этих позиций. Предварительно для данной работы проведен поиск достаточно точных критериев обнаружения ДНК-активности антител иммуноглобулина (Ig) G из сыворотки крови больных КЭ и здоровых доноров. Показано, что относительная активность антител IgG значительно варьирует у пациентов, но большинство образцов (91%) имели определяемый уровень ДНКазной активности. Поликлональные ДНКазные антитела IgG не активировались в присутствии ЭДТА или после диализа с ЭДТА, но могли активироваться некоторыми добавленными ионами металлов с уровнем активности, уменьшающимся в ряду Mn2+ + Ca2+ ≥ Mn2+ + Mg2+ ≥ Mn2+ ≥ Mg2+ + Ca2+ ≥ Co2+ ≥ Mg2+ > Ca2+, в то время как K+ , Na+ , Ni2+, Zn2+ и Cu2+ не стимулировали гидролиз ДНК. Аффинная хроматография на ДНК-целлюлозе разделила ДНКазные антитела IgG на множество субфракций с различным сродством к ДНК и очень разными уровнями относительной активности. Возможные причины каталитического разнообразия поликлональных человеческих аутоантител обсуждаются.

Several autoimmune diseases with chronic clinical course are characterized by detection of DNA autoantibodies in patients’ serum, while there are no such IgGs in healthy donors’ blood or in patients with acute clinical course with no evidence of chronization. Tick-borne encephalitis has not been considered this way. Several strict criteria have been applied to show that the DNase activity is an intrinsic property of IgGs from the sera of TBE patients but not from healthy donors. The relative activity of IgGs has been shown to vary extensively from patient to patient, but most of the preparations (91%) had detectable levels of the DNase activity. Polyclonal DNase IgGs were not active in the presence of EDTA or after a dialysis against EDTA, but could be activated by several externally added metal ions, with the level of activity decreasing in the order Mn2+ + Ca2+ ≥ Mn2+ + Mg2+ ≥ Mn2+ ≥ ≥ Mg2+ + Ca2+ ≥ Ca2+ ≥ Mg2+ > Ca2+, while K+ , Na+ , Ni2+, Zn2+, and Cu2+ did not stimulate DNA hydrolysis. Affinity chromatography on DNA-cellulose separated the DNase IgGs into many subfractions with various affinities for DNA and very different levels of the relative activity. Possible reasons for catalytic diversity of polyclonal human Abzs are discussed.

вирусные инфекцииклещевой энцефалитабзимыгидролиз ДНК

viral infectionstick-borne encephalitisabzymesDNA hydrolysis

Исследование осуществлено при поддержке гранта аналитической ведомственной целевой программы «Развитие научного потенциала высшей школы» (№ 2.1.1/5580); гранта Президиума РАН (программа «Молекулярная и клеточная биология», № 22.7, «Фундаментальные науки — медицине», № 21.16), грантов РФФИ (07-04-00387) и РФФИБФФИ (08-04-90014) и при финансовой поддержке СО РАН.

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The authors declare that there are no conflicts of interest present.