ssmuБюллетень сибирской медициныBulletin of Siberian Medicine1682-03631819-3684Siberian State Medical University, the Ministry of Healthcare of the Russian Federation10.20538/1682-0363-2019-1-228-236ssmu-2188Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL PAPERSМоноциты, β-лимфоциты и дендритные клетки при риновирусиндуцированном обострении бронхиальной астмыMonocytes, B-cells and dendritic cells during rhinovirus-induced asthma exacerbationhttps://orcid.org/0000-0003-3064-8871ХаитовР. М.KhaitovR. M.

Хаитов Рахим Мусаевич, доктор медицинских наук, профессор, академик РАН, научный руководитель Института иммунологии

115478, г. Москва, Каширское шоссе, 24

Khaitov Rahim M., DМ, Professor, Аcademician RAS, Research Advisor of Institute of Immunology

24, Kashirskoe Sh., 115478, Moscow

https://orcid.org/0000-0001-9610-0935НиконоваА. А.NikonovaA. A.

Никонова Александра Александровна, кандидат биологических наук, заведующий лабораторией, НИИВС им. И.И. Мечникова

115478, г. Москва, Каширское шоссе, 24, 

105064, г. Москва, Малый Казенный пер., 5а

Nikonova Alexandra A., PhD, Нead of the Laboratory, Mechnikov Research Institute for Vaccines and Sera

24, Kashirskoe Sh., 115478, Moscow, 

5a, Malyj Kazennyj Per., 105064, Moscow

aa.nikonova@nrcii.ruhttps://orcid.org/0000-0003-4961-9640ХаитовМ. Р.KhaitovM. R.

Хаитов Муса Рахимович, доктор медицинских наук, профессор, член-корреспондент РАН, директор Института иммунологии

115478, г. Москва, Каширское шоссе, 24

Khaitov Musa R., DМ, Professor, Corresponding Member of RAS, Director of Institute of Immunology

24, Kashirskoe Sh., 115478, Moscow

ГНЦ Институт иммунологииРоссияInstitute of ImmunologyRussian FederationГНЦ Институт иммунологии; Научно-исследовательский институт вакцин и сывороток (НИИВС) им. И.И. МечниковаРоссияInstitute of Immunology; Mechnikov Research Institute for Vaccines and SeraRussian Federation201916052019181228236Copyright © Хаитов Р.М., Никонова А.А., Хаитов М.Р., 20192019Хаитов Р.М., Никонова А.А., Хаитов М.Р.Khaitov R.M., Nikonova A.A., Khaitov M.R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://bulletin.ssmu.ru/jour/article/view/2188Цель

Цель. Цель работы заключалась в определении количества различных популяций клеток периферической крови у пациентов с бронхиальной астмой (БА) и здоровых индивидуумов до и во время экспериментальной риновирусной инфекции.

Материалы и методы

Материалы и методы. Экспериментальная инфекция риновирусом 16-го типа (РВ16) была индуцирована у добровольцев, не имеющих антител к РВ16. Мононуклеары периферической крови от 8 пациентов с легкой персистирующей БА (LPA), 12 с персистирующей БА средней тяжести (PAST) и 6 здоровых добровольцев (норма), полученные за 14 сут до заражения и на 4-е сут после заражения, исследовались методом проточной цитометрии. B-клетки идентифицировали как CD19+. Моноциты классифицировали как CD19–, MHCII, CD14high. Клетки, охарактеризованные как MHCII, CD14neg-low, далее классифицировали как плазмоцитоидные (pDC) (CD123+) и миелоидные (mDC) дендритные клетки (CD11chigh).

Результаты

Результаты. Не выявлено различий между разными группами добровольцев по содержанию моноцитов, mDC и pDC в образцах крови до инфицирования. Однако мы обнаружили повышение уровня B-клеток в крови пациентов с БА по сравнению со здоровыми добровольцами (p < 0,05). Также на 4-е сут после заражения РВ16 мы выявили снижение количества циркулирующих pDC в крови пациентов с БА (LPA и PAST) по сравнению с исходным уровнем (p < 0,05).

Заключение

Заключение. Полученные данные свидетельствуют о повышенном миграционным потенциале циркулирующих pDC при обострении вирус-индуцированных осложнений БА. Отсутствие изменений в количестве mDC во время инфекции мы объясняем тем, что разные популяции DC мигрируют к участкам аллергического воспаления в разные временные интервалы.

Aim

Aim. In this study we aimed to investigate circulating blood cells during experimental virus-induced asthma exacerbation vs baseline.

Materials and methods

Materials and methods. Rhinovirus 16 (RV16) experimental infections were induced in RV16-seronegative moderate and mild atopic asthmatic and healthy non-atopic subjects. PBMC from 8 mild, 12 moderate asthmatics and 6 normal subjects obtained at baseline (14 day) and at day 4 after infection with RV16 were analyzed by flow cytometry. B-cells were identified as CD19+. Monocytes were identified as MHC II, CD14high cells. The MHC II, CD14neg-low cells were further classified by CD123 and CD11c expression into myeloid DC (CD11chigh, mDC), plasmacytoid DC (CD123+, pDC).

Results

Results. There were no differences at baseline in frequencies of blood monocytes, mDC and pDC in asthmatic compared to normal subjects, but we found increased amount of B-cells in asthma group (p < 0.05). At day 4 after RV16 infection we found decreased percentages of pDC in both moderate and mild asthmatics (p < 0.05) compared to baseline.

Conclusion

Conclusion. These data suggest an increased migratory potential of circulating pDCs during virus-induced asthma exacerbation. In patients with asthma pDCs could be recruited to the airways. It is possible that the distinct subsets of DCs may be recruited at different time points to the effector sites of allergic inflammation.

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The authors declare that there are no conflicts of interest present.