ssmuБюллетень сибирской медициныBulletin of Siberian Medicine1682-03631819-3684Siberian State Medical University, the Ministry of Healthcare of the Russian Federation10.20538/1682-0363-2019-2-119-126ssmu-2313Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL PAPERSПролиферация и апоптоз лимфоцитов у экспериментальных животных после многократной трансплантации клеток иммунной системы, проведенной в ювенильный период развитияProliferation and apoptosis of experimental animal’s lymphocytes after multiple transplantation of immune cells from opposite behavioral types of donors carried out in the juvenile periodМарковаЕ. В.MarkovaE. V.evgeniya_markova@mail.ruСавкинИ. В.AnikeevaO. S.

Anikeeva Olga S., Junior Researcher, Neuroimmunology Laboratory

14, Yadrintsevskaya Str., 630099

АникееваО. С.SavkinI. V.КозловВ. А.KozlovV. A.Научно-исследовательский институт фундаментальной и клинической иммунологии (НИИФКИ)РоссияResearch Institute of Fundamental and Clinical Immunology (RIFCI)Russian Federation201911082019182119126Copyright © Маркова Е.В., Савкин И.В., Аникеева О.С., Козлов В.А., 20192019Маркова Е.В., Савкин И.В., Аникеева О.С., Козлов В.А.Markova E.V., Anikeeva O.S., Savkin I.V., Kozlov V.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://bulletin.ssmu.ru/jour/article/view/2313

Цель исследования – определение субпопуляционного состава, пролиферативной активности и уровня апоптоза Т-лимфоцитов у половозрелых мышей-реципиентов, подвергнутых в ювенильный период развития многократной трансплантации иммунных клеток с различными функциональными показателями, характерными для животных с оппозитными типами поведения. Материалы и методы. Исследования проведены на самцах мышей (CBAxC57BL/6) F1 (n = 190), которым, начиная с 4–5-недельного возраста, была проведена трехкратная трансплантация иммунных клеток с определенными функциональными характеристиками. У сингенных реципиентов в половозрелом возрасте проводилось фенотипирование клеток селезенки методом проточной цитофлуорометрии с моноклональными антителами против CD3+, СD4+, CD8+, оценивались пролиферативная активность и апоптоз спленоцитов. Результаты. Животные, подвергнутые в ювенильный период развития многократной трансплантации иммунных клеток от сингенных доноров с оппозитными типами поведения, в половозрелом возрасте характеризуются различными функциональными свойствами лимфоцитов селезенки. Причем наиболее выраженные изменения выявлены у животных, получивших трансплантат от доноров с пассивным типом поведения, лимфоциты которых характеризовались относительно низкой пролиферативной активностью и чувствительностью к Т-клеточному митогену, снижением доли апоптоза CD4+, CD8 + клеток, находящихся в условиях обедненной культуральной среды и повышением уровня активационного и дексаметазон-индуцированного апоптоза CD4+ лимфоцитов.

The aim of the study was to determine subpopulation content, proliferative activity and T-lymphocyte apoptosis level in adult mice-recipients that in the juvenile period underwent multiple transplantation of immune cells with different functional properties from opposite behavioral types of donors. Materials and methods. The study was carried out on male mice (CBAxC57BL/6) F1 (n = 190), which underwent transplantation of immune cells with definite functional properties three times starting from the age of 4–5-weeks. Phenotyping of recipients’ spleen cells was carried out by flow cytometry with monoclonal antibodies against CD3+, СD4+, CD8+. Splenocyte proliferation and apoptosis were estimated. Results. Animals that underwent threefold transplantation of immune cells from singeing opposite behavioral types of donors in the juvenile period revealed different functional properties of spleen lymphocytes in adults. The most pronounced changes were detected in the animals receiving the immune cells from donors with passive behavior type. The lymphocytes of these recipients were characterized by relatively low proliferative activity and T-mytogen sensitivity, decreased CD4+, CD8+ apoptosis level under deficient medium conditions and increased level of activation and dexamethasone-induced apoptosis in CD4+ lymphocytes.

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The authors declare that there are no conflicts of interest present.