ssmuБюллетень сибирской медициныBulletin of Siberian Medicine1682-03631819-3684Siberian State Medical University, the Ministry of Healthcare of the Russian Federation10.20538/1682-0363-2020-2-195-203ssmu-2877Research ArticleОБЗОРЫ И ЛЕКЦИИREVIEW AND LECTURESОптогенетические методы и технологии в решении прикладных медицинских задачOptogenetic methods and technologies in solving applied medical problemshttps://orcid.org/0000-0002-1862-6816СорокинаЛ. Е.SorokinaL. E.leya.sorokina@mail.ruhttps://orcid.org/0000-0001-9451-1757ПетренкоВ. И.PetrenkoV. I.https://orcid.org/0000-0002-0521-0619СубботкинМ. В.SubbotkinM. V.https://orcid.org/0000-0001-7553-5382КулановаА. А.KulanovaA. A.https://orcid.org/0000-0002-5861-190XКучеренкоА. С.KucherenkoA. S.https://orcid.org/0000-0002-1309-4005КубышкинА. В.KubyshkinA. V.

д-р мед. наук, профессор, зав. кафедрой общей и клинической патофизиологии

Россия, Республика Крым, 295051, г. Симферополь, бул. Ленина, 5/7

https://orcid.org/0000-0003-3065-5748ФомочкинаИ. И.FomochkinaI. I.https://orcid.org/0000-0002-8247-4773НомеровскаяА. Ю.NomerovskayaA. Yu.

ассистент, кафедра общей и клинической патофизиологии

Россия, Республика Крым, 295051, г. Симферополь, бул. Ленина, 5/7

https://orcid.org/0000-0002-1682-9441ХалиловС. С.HalilovS. I.

4, Academician Vernadsky Str., Simferopol, 295007, Republic of Crimea, Russian Federation

Медицинская академия имени С.И. Георгиевского, Крымский федеральный университет (КФУ) имени В.И. ВернадскогоРоссияV.I. Vernadsky Crimean Federal University, Medical Academy named after S.I. GeorgievskyRussian FederationФизико-технический институт, Крымский федеральный университет (КФУ) имени В.И. ВернадскогоРоссияV.I. Vernadsky Crimean Federal University, Physics and Technology InstituteRussian Federation202012072020192195203Copyright © Сорокина Л.Е., Петренко В.И., Субботкин М.В., Куланова А.А., Кучеренко А.С., Кубышкин А.В., Фомочкина И.И., Номеровская А.Ю., Халилов С.С., 20202020Сорокина Л.Е., Петренко В.И., Субботкин М.В., Куланова А.А., Кучеренко А.С., Кубышкин А.В., Фомочкина И.И., Номеровская А.Ю., Халилов С.С.Sorokina L.E., Petrenko V.I., Subbotkin M.V., Kulanova A.A., Kucherenko A.S., Kubyshkin A.V., Fomochkina I.I., Nomerovskaya A.Y., Halilov S.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://bulletin.ssmu.ru/jour/article/view/2877оптогенетикаопсиныионные каналыоптоволоконные системыфотостимуляциянейроинтерфейсоптогенетическая терапияoptogeneticsopsinsion channelsfiber optic systemsphotostimulationneurointerfaceoptogenetic therapyИсследования были частично поддержаны Программой развития ФГАОУ ВО «Крымский федеральный университет В.И. Вернадского» на 2015–2024 г.This work was partially supported by the V.I. Vernadsky Crimean Federal University Development Program for 2015–2024.ReferencesOesterhelt D., Stoeckenius W. Rhodopsin-like protein from the purple membrane of Halobacterium halobium. Nat. New Biol. 1971; 233 (39): 149–152. DOI: 10.1038/newbio233149a0.Oesterhelt D., Stoeckenius W. Rhodopsin-like protein from the purple membrane of Halobacterium halobium. Nat. New Biol. 1971; 233 (39): 149–152. DOI: 10.1038/newbio233149a0.Crick F.H. Thinking about the brain. Sci. Am. 1979; 241 ( 3): 219–232. DOI: 10.1038/scientificamerican0979-219.Crick F.H. Thinking about the brain. Sci. Am. 1979; 241 ( 3): 219–232. DOI: 10.1038/scientificamerican0979-219.Boyden E.S., Zhang F., Bamberg E., Nagel G., Deisseroth K. Millisecond-timescale, genetically targeted optical control of neural activity. Nat. Neurosci. 2005; 8 (9): 1263–1268. DOI: 10.1038/nn1525.Boyden E.S., Zhang F., Bamberg E., Nagel G., Deisseroth K. Millisecond-timescale, genetically targeted optical control of neural activity. Nat. Neurosci. 2005; 8 (9): 1263–1268. DOI: 10.1038/nn1525.Zhang F., Prigge M., Beyrière F., Tsunoda S.P., Mattis J., Yizhar O., Hegemann P., Deisseroth K. Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri. Nat. Neurosci. 2008; 11 (6): 631–633. DOI: 10.1038/nn.2120.Zhang F., Prigge M., Beyrière F., Tsunoda S.P., Mattis J., Yizhar O., Hegemann P., Deisseroth K. Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri. Nat. Neurosci. 2008; 11 (6): 631–633. DOI: 10.1038/nn.2120.Deubner J., Coulon P., Diester I. Optogenetic approaches to study the mammalian brain. Curr. Opin. Struct. Biol. 2019; 57: 157–163. DOI: 10.1016/j.sbi.2019.04.003.Deubner J., Coulon P., Diester I. Optogenetic approaches to study the mammalian brain. Curr. Opin. Struct. Biol. 2019; 57: 157–163. DOI: 10.1016/j.sbi.2019.04.003.Dimidschstein J., Chen Q., Tremblay R. et al. A viral strategy for targeting and manipulating interneurons across vertebrate species. Nat. Neurosci. 2016; 19 (12): 1743–1749. DOI: 10.1038/nn.4430.Dimidschstein J., Chen Q., Tremblay R. et al. A viral strategy for targeting and manipulating interneurons across vertebrate species. Nat. Neurosci. 2016; 19 (12): 1743–1749. DOI: 10.1038/nn.4430.Fenno L.E., Mattis J., Ramakrishnan C. et al. Targeting cells with single vectors using multiple-feature Boolean logic. Nat. Methods. 2014; 11 (7): 763–772. DOI: 10.1038/nmeth.2996.Fenno L.E., Mattis J., Ramakrishnan C. et al. Targeting cells with single vectors using multiple-feature Boolean logic. Nat. Methods. 2014; 11 (7): 763–772. DOI: 10.1038/nmeth.2996.Arenkiel B.R., Peca J., Davison I.G. et al. In vivo light-induced activation of neural circuitry in transgenic mice expressing channelrhodopsin-2. Neuron. 2007; 54 (2): 205–218. DOI: 10.1016/j.neuron.2007.03.005.Arenkiel B.R., Peca J., Davison I.G. et al. In vivo light-induced activation of neural circuitry in transgenic mice expressing channelrhodopsin-2. Neuron. 2007; 54 (2): 205–218. DOI: 10.1016/j.neuron.2007.03.005.Zeng H., Madisen L. Mouse transgenic approaches in optogenetics. Prog. Brain Res. 2012; 196: 193–213. DOI: 10.1016/B978-0-444-59426-6.00010-0.Zeng H., Madisen L. Mouse transgenic approaches in optogenetics. Prog. Brain Res. 2012; 196: 193–213. DOI: 10.1016/B978-0-444-59426-6.00010-0.Park J.E., Silva A.C. Generation of genetically engineered non-human primate models of brain function and neurological disorders. Am. J. Primatol. 2019; 81 (2): e22931. DOI: 10.1002/ajp.22931.Park J.E., Silva A.C. Generation of genetically engineered non-human primate models of brain function and neurological disorders. Am. J. Primatol. 2019; 81 (2): e22931. DOI: 10.1002/ajp.22931.Bitzenhofer S.H., Ahlbeck J., Wolff A. et al. Layer-specific optogenetic activation of pyramidal neurons causes beta-gamma entrainment of neonatal networks. Nat. Commun. 2017; 8: 14563. DOI: 10.1038/ncomms14563.Bitzenhofer S.H., Ahlbeck J., Wolff A. et al. Layer-specific optogenetic activation of pyramidal neurons causes beta-gamma entrainment of neonatal networks. Nat. Commun. 2017; 8: 14563. DOI: 10.1038/ncomms14563.Adesnik H., Scanziani M. Lateral competition for cortical space by layer-specific horizontal circuits. Nature. 2010; 464 (7292): 1155–1160. DOI: 10.1038/nature08935.Adesnik H., Scanziani M. Lateral competition for cortical space by layer-specific horizontal circuits. Nature. 2010; 464 (7292): 1155–1160. DOI: 10.1038/nature08935.Han X., Chow B.Y., Zhou H., Klapoetke N.C., Chuong A., Rajimehr R., Yang A., Baratta M.V., Winkle J., Desimone R., Boyden E.S. A high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex. Front. Syst. Neurosci. 2011; 5: 18. DOI: 10.3389/fnsys.2011.00018.Han X., Chow B.Y., Zhou H., Klapoetke N.C., Chuong A., Rajimehr R., Yang A., Baratta M.V., Winkle J., Desimone R., Boyden E.S. A high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex. Front. Syst. Neurosci. 2011; 5: 18. DOI: 10.3389/fnsys.2011.00018.Jing M., Zhang P., Wang G. et al. A genetically encoded fluorescent acetylcholine indicator for in vitro and in vivo studies. Nat. Biotechnol. 2018; 36 (8): 726–737. DOI: 10.1038/nbt.4184.Jing M., Zhang P., Wang G. et al. A genetically encoded fluorescent acetylcholine indicator for in vitro and in vivo studies. Nat. Biotechnol. 2018; 36 (8): 726–737. DOI: 10.1038/nbt.4184.Srinivasan S., Hosokawa T., Vergara P. et al. Miniaturized microscope with flexible light source input for neuronal imaging and manipulation in freely behaving animals. Biochem. Biophys. Res. Commun. 2019; 517 (3): 520–524. DOI: 10.1016/j.bbrc.2019.07.082.Srinivasan S., Hosokawa T., Vergara P. et al. Miniaturized microscope with flexible light source input for neuronal imaging and manipulation in freely behaving animals. Biochem. Biophys. Res. Commun. 2019; 517 (3): 520–524. DOI: 10.1016/j.bbrc.2019.07.082.Hägglund M., Borgius L., Dougherty K.J., Kiehn O. Activation of groups of excitatory neurons in the mammalian spinal cord or hindbrain evokes locomotion. Nat. Neurosci. 2010; 13 (2): 246–252. DOI: 10.1038/nn.2482.Hägglund M., Borgius L., Dougherty K.J., Kiehn O. Activation of groups of excitatory neurons in the mammalian spinal cord or hindbrain evokes locomotion. Nat. Neurosci. 2010; 13 (2): 246–252. DOI: 10.1038/nn.2482.Farmer D.G., Pracejus N., Dempsey B. et al. On the presence and functional significance of sympathetic premotor neurons with collateralized spinal axons in the rat. J. Physiol. 2019; 597 (13): 3407–3423. DOI: 10.1113/JP277661.Farmer D.G., Pracejus N., Dempsey B. et al. On the presence and functional significance of sympathetic premotor neurons with collateralized spinal axons in the rat. J. Physiol. 2019; 597 (13): 3407–3423. DOI: 10.1113/JP277661.Miterko L.N., Baker K.B. et al. Consensus paper: experimental neurostimulation of the cerebellum. Cerebellum. 2019; 18 (6): 1064–1097. DOI: 10.1007/s12311-019-01041-5.Miterko L.N., Baker K.B. et al. Consensus paper: experimental neurostimulation of the cerebellum. Cerebellum. 2019; 18 (6): 1064–1097. DOI: 10.1007/s12311-019-01041-5.Liu K., Wang L. Optogenetics: Therapeutic spark in neuropathic pain. Bosn. J. Basic. Med. Sci. 2019; 19 (4): 321–327. DOI: 10.17305/bjbms.2019.4114.Liu K., Wang L. Optogenetics: Therapeutic spark in neuropathic pain. Bosn. J. Basic. Med. Sci. 2019; 19 (4): 321–327. DOI: 10.17305/bjbms.2019.4114.Kastanenka K.V., Calvo-Rodriguez M., Hou S.S. et al. Frequency-dependent exacerbation of Alzheimer’s disease neuropathophysiology. Sci. Rep. 2019; 9 (1): 8964. DOI: 10.1038/s41598-019-44964-z.Kastanenka K.V., Calvo-Rodriguez M., Hou S.S. et al. Frequency-dependent exacerbation of Alzheimer’s disease neuropathophysiology. Sci. Rep. 2019; 9 (1): 8964. DOI: 10.1038/s41598-019-44964-z.Krook-Magnuson E., Armstrong C., Oijala M., Soltesz I. On-demand optogenetic control of spontaneous seizures in temporal lobe epilepsy. Nat. Commun. 2013; 4: 1376. DOI: 10.1038/ncomms2376.Krook-Magnuson E., Armstrong C., Oijala M., Soltesz I. On-demand optogenetic control of spontaneous seizures in temporal lobe epilepsy. Nat. Commun. 2013; 4: 1376. DOI: 10.1038/ncomms2376.Deisseroth K. Optogenetics and psychiatry: applications, challenges, and opportunities. Biol. Psychiatry. 2012; 71 (12): 1030–1032. DOI: 10.1016/j.biopsych.2011.12.021.Deisseroth K. Optogenetics and psychiatry: applications, challenges, and opportunities. Biol. Psychiatry. 2012; 71 (12): 1030–1032. DOI: 10.1016/j.biopsych.2011.12.021.Shirai F., Hayashi-Takagi A. Optogenetics: Applications in psychiatric research. Psychiatry Clin. Neurosci. 2017; 71 (6): 363–372. DOI: 10.1111/pcn.12516.Shirai F., Hayashi-Takagi A. Optogenetics: Applications in psychiatric research. Psychiatry Clin. Neurosci. 2017; 71 (6): 363–372. DOI: 10.1111/pcn.12516.Tye K.M., Mirzabekov J.J., Warden M.R. et al. Dopamine neurons modulate neural encoding and expression of depression-related behaviour. Nature. 2013; 493 (7433): 537–541. DOI: 10.1038/nature11740.Tye K.M., Mirzabekov J.J., Warden M.R. et al. Dopamine neurons modulate neural encoding and expression of depression-related behaviour. Nature. 2013; 493 (7433): 537–541. DOI: 10.1038/nature11740.Bass C.E., Grinevich V.P., Gioia D. et al. Optogenetic stimulation of VTA dopamine neurons reveals that tonic but not phasic patterns of dopamine transmission reduce ethanol self-administration. Front. Behav. Neurosci. 2013; 7: 173. DOI: 10.3389/fnbeh.2013.00173.Bass C.E., Grinevich V.P., Gioia D. et al. Optogenetic stimulation of VTA dopamine neurons reveals that tonic but not phasic patterns of dopamine transmission reduce ethanol self-administration. Front. Behav. Neurosci. 2013; 7: 173. DOI: 10.3389/fnbeh.2013.00173.Burguière E., Monteiro P., Feng G., Graybiel A.M. Optogenetic stimulation of lateral orbitofronto-striatal pathway suppresses compulsive behaviors. Science. 2013; 340 (6137): 1243–1246. DOI: 10.1126/science.1232380.Burguière E., Monteiro P., Feng G., Graybiel A.M. Optogenetic stimulation of lateral orbitofronto-striatal pathway suppresses compulsive behaviors. Science. 2013; 340 (6137): 1243–1246. DOI: 10.1126/science.1232380.Henriksen B.S., Marc R.E., Bernstein P.S. Optogenetics for retinal disorders. J. Ophthalmic. Vis. Res. 2014; 9 (3): 374–372. DOI:10.4103/2008-322X.143379.Henriksen B.S., Marc R.E., Bernstein P.S. Optogenetics for retinal disorders. J. Ophthalmic. Vis. Res. 2014; 9 (3): 374–372. DOI:10.4103/2008-322X.143379.Berry M.H., Holt A., Salari A. et al. Restoration of high-sensitivity and adapting vision with a cone opsin. Nat. Commun. 2019; 10 (1): 1221. DOI: 10.1038/s41467-019-09124-x.Berry M.H., Holt A., Salari A. et al. Restoration of high-sensitivity and adapting vision with a cone opsin. Nat. Commun. 2019; 10 (1): 1221. DOI: 10.1038/s41467-019-09124-x.Ganjawala T.H., Lu Q., Fenner M.D., Abrams G.W., Pan Z.H. Improved CoChR variants restore visual acuity and contrast sensitivity in a mouse model of blindness under ambient light сonditions. Mol. Ther. 2019; 27 (6): 1195–1205. DOI: 10.1016/j.ymthe.2019.04.002.Ganjawala T.H., Lu Q., Fenner M.D., Abrams G.W., Pan Z.H. Improved CoChR variants restore visual acuity and contrast sensitivity in a mouse model of blindness under ambient light сonditions. Mol. Ther. 2019; 27 (6): 1195–1205. DOI: 10.1016/j.ymthe.2019.04.002.Hernandez V.H., Gehrt A., Jing Z. et al. Optogenetic stimulation of the auditory nerve. J. Vis. Exp. 2014; (92): e52069/ DOI: 10.3791/52069.Hernandez V.H., Gehrt A., Jing Z. et al. Optogenetic stimulation of the auditory nerve. J. Vis. Exp. 2014; (92): e52069/ DOI: 10.3791/52069.Hernandez V.H., Gehrt A., Reuter K. et al. Optogenetic stimulation of the auditory pathway. J. Clin. Invest. 2014; 124 (3): 1114–1129. DOI: 10.1172/JCI69050.Hernandez V.H., Gehrt A., Reuter K. et al. Optogenetic stimulation of the auditory pathway. J. Clin. Invest. 2014; 124 (3): 1114–1129. DOI: 10.1172/JCI69050.Wrobel C., Dieter A., Huet A. et al. Optogenetic stimulation of cochlear neurons activates the auditory pathway and restores auditory-driven behavior in deaf adult gerbils. Sci. Transl. Med. 2018; 10 (449): eaao4496. DOI: 10.1126/scitranslmed.aao0540.Wrobel C., Dieter A., Huet A. et al. Optogenetic stimulation of cochlear neurons activates the auditory pathway and restores auditory-driven behavior in deaf adult gerbils. Sci. Transl. Med. 2018; 10 (449): eaao4496. DOI: 10.1126/scitranslmed.aao0540.Ye H., Daoud E., Baba M., Peng R.W., Fussenegger M. A synthetic optogenetic transcription device enhances blood-glucose homeostasis in mice. Science. 2011; 332 (6037): 1565–1568. DOI: 10.1126/science.1203535.Wrobel C., Dieter A., Huet A. et al. Optogenetic stimulation of cochlear neurons activates the auditory pathway and restores auditory-driven behavior in deaf adult gerbils. Sci. Transl. Med. 2018; 10 (449): eaao4496. DOI: 10.1126/scitranslmed.aao0540.Shao J., Xue S., Yu G. et al. Smartphone-controlled optogenetically engineered cells enable semiautomatic glucose homeostasis in diabetic mice. Sci. Transl. Med. 2017; 9 (387): eaal2298. DOI: 10.1126/scitranslmed.aal2298.Ye H., Daoud E., Baba M., Peng R.W., Fussenegger M. A synthetic optogenetic transcription device enhances blood-glucose homeostasis in mice. Science. 2011; 332 (6037): 1565–1568. DOI: 10.1126/science.1203535.Yu L., Zhou L., Cao G. et al. Optogenetic modulation of cardiac sympathetic nerve activity to prevent ventricular arrhythmias. J. Am. Coll. Cardiol. 2017; 70 (22): 2778–2790. DOI: 10.1016/j.jacc.2017.09.1107.Ye H., Daoud E., Baba M., Peng R.W., Fussenegger M. A synthetic optogenetic transcription device enhances blood-glucose homeostasis in mice. Science. 2011; 332 (6037): 1565–1568. DOI: 10.1126/science.1203535.Majumder R., Feola I., Teplenin A.S., de Vries A.A., Panfilov A.V., Pijnappels D.A. Optogenetics enables real-time spatiotemporal control over spiral wave dynamics in an excitable cardiac system. Elife. 2018; 7: e41076. DOI: 10.7554/eLife.41076.Shao J., Xue S., Yu G. et al. Smartphone-controlled optogenetically engineered cells enable semiautomatic glucose homeostasis in diabetic mice. Sci. Transl. Med. 2017; 9 (387): eaal2298. DOI: 10.1126/scitranslmed.aal2298.Cheng Y., Li H., Lei H. et al. Flexible and precise control of cardiac rhythm with blue light. Biochem. Biophys. Res. Commun. 2019; 514 (3): 759–764. DOI: 10.1016/j.bbrc.2019.05.035.Shao J., Xue S., Yu G. et al. Smartphone-controlled optogenetically engineered cells enable semiautomatic glucose homeostasis in diabetic mice. Sci. Transl. Med. 2017; 9 (387): eaal2298. DOI: 10.1126/scitranslmed.aal2298.Quiñonez Uribe R.A., Luther S., Diaz-Maue L., Richter C. Energy-reduced arrhythmia termination using global photostimulation in optogenetic murine hearts. Front. Physiol. 2018; 9: 1651. DOI: 10.3389/fphys.2018.01651.Yu L., Zhou L., Cao G. et al. Optogenetic modulation of cardiac sympathetic nerve activity to prevent ventricular arrhythmias. J. Am. Coll. Cardiol. 2017; 70 (22): 2778–2790. DOI: 10.1016/j.jacc.2017.09.1107.Zhang H., Cohen A.E. Optogenetic approaches to drug discovery in neuroscience and beyond. Trends Biotechnol. 2017; 35 (7): 625–639. DOI: 10.1016/j.tibtech.2017.04.002.Yu L., Zhou L., Cao G. et al. Optogenetic modulation of cardiac sympathetic nerve activity to prevent ventricular arrhythmias. J. Am. Coll. Cardiol. 2017; 70 (22): 2778–2790. DOI: 10.1016/j.jacc.2017.09.1107.Kiełbus M., Czapiński J., Odrzywolski A. et al. Optogenetics in cancer drug discovery. Expert Opin. Drug Discov. 2018; 13 (5): 459–472. DOI: 10.1080/17460441.2018.1437138.Majumder R., Feola I., Teplenin A.S., de Vries A.A., Panfilov A.V., Pijnappels D.A. Optogenetics enables real-time spatiotemporal control over spiral wave dynamics in an excitable cardiac system. Elife. 2018; 7: e41076. DOI: 10.7554/eLife.41076.Sahel J.-A., Roska B. Gene therapy for blindness. Ann. Rev. Neurosci. 2013; 36: 467–488. DOI: 10.1146/annurev-neuro-062012-170304.Majumder R., Feola I., Teplenin A.S., de Vries A.A., Panfilov A.V., Pijnappels D.A. Optogenetics enables real-time spatiotemporal control over spiral wave dynamics in an excitable cardiac system. Elife. 2018; 7: e41076. DOI: 10.7554/eLife.41076.Cheng Y., Li H., Lei H. et al. Flexible and precise control of cardiac rhythm with blue light. Biochem. Biophys. Res. Commun. 2019; 514 (3): 759–764. DOI: 10.1016/j.bbrc.2019.05.035.Cheng Y., Li H., Lei H. et al. Flexible and precise control of cardiac rhythm with blue light. Biochem. Biophys. Res. Commun. 2019; 514 (3): 759–764. DOI: 10.1016/j.bbrc.2019.05.035.Cheng Y., Li H., Lei H. et al. Flexible and precise control of cardiac rhythm with blue light. Biochem. Biophys. Res. Commun. 2019; 514 (3): 759–764. DOI: 10.1016/j.bbrc.2019.05.035.Cheng Y., Li H., Lei H. et al. Flexible and precise control of cardiac rhythm with blue light. Biochem. Biophys. Res. Commun. 2019; 514 (3): 759–764. DOI: 10.1016/j.bbrc.2019.05.035.Quiñonez Uribe R.A., Luther S., Diaz-Maue L., Richter C. Energy-reduced arrhythmia termination using global photostimulation in optogenetic murine hearts. Front. Physiol. 2018; 9: 1651. DOI: 10.3389/fphys.2018.01651.Quiñonez Uribe R.A., Luther S., Diaz-Maue L., Richter C. Energy-reduced arrhythmia termination using global photostimulation in optogenetic murine hearts. Front. Physiol. 2018; 9: 1651. DOI: 10.3389/fphys.2018.01651.Quiñonez Uribe R.A., Luther S., Diaz-Maue L., Richter C. Energy-reduced arrhythmia termination using global photostimulation in optogenetic murine hearts. Front. Physiol. 2018; 9: 1651. DOI: 10.3389/fphys.2018.01651.Quiñonez Uribe R.A., Luther S., Diaz-Maue L., Richter C. Energy-reduced arrhythmia termination using global photostimulation in optogenetic murine hearts. Front. Physiol. 2018; 9: 1651. DOI: 10.3389/fphys.2018.01651.Zhang H., Cohen A.E. Optogenetic approaches to drug discovery in neuroscience and beyond. Trends Biotechnol. 2017; 35 (7): 625–639. DOI: 10.1016/j.tibtech.2017.04.002.Zhang H., Cohen A.E. Optogenetic approaches to drug discovery in neuroscience and beyond. Trends Biotechnol. 2017; 35 (7): 625–639. DOI: 10.1016/j.tibtech.2017.04.002.Zhang H., Cohen A.E. Optogenetic approaches to drug discovery in neuroscience and beyond. Trends Biotechnol. 2017; 35 (7): 625–639. DOI: 10.1016/j.tibtech.2017.04.002.Zhang H., Cohen A.E. Optogenetic approaches to drug discovery in neuroscience and beyond. Trends Biotechnol. 2017; 35 (7): 625–639. DOI: 10.1016/j.tibtech.2017.04.002.Kiełbus M., Czapiński J., Odrzywolski A. et al. Optogenetics in cancer drug discovery. Expert Opin. Drug Discov. 2018; 13 (5): 459–472. DOI: 10.1080/17460441.2018.1437138.Kiełbus M., Czapiński J., Odrzywolski A. et al. Optogenetics in cancer drug discovery. Expert Opin. Drug Discov. 2018; 13 (5): 459–472. DOI: 10.1080/17460441.2018.1437138.Kiełbus M., Czapiński J., Odrzywolski A. et al. Optogenetics in cancer drug discovery. Expert Opin. Drug Discov. 2018; 13 (5): 459–472. DOI: 10.1080/17460441.2018.1437138.Kiełbus M., Czapiński J., Odrzywolski A. et al. Optogenetics in cancer drug discovery. Expert Opin. Drug Discov. 2018; 13 (5): 459–472. DOI: 10.1080/17460441.2018.1437138.Sahel J.-A., Roska B. Gene therapy for blindness. Ann. Rev. Neurosci. 2013; 36: 467–488. DOI: 10.1146/annurev-neuro-062012-170304.Sahel J.-A., Roska B. Gene therapy for blindness. Ann. Rev. Neurosci. 2013; 36: 467–488. DOI: 10.1146/annurev-neuro-062012-170304.Sahel J.-A., Roska B. Gene therapy for blindness. Ann. Rev. Neurosci. 2013; 36: 467–488. DOI: 10.1146/annurev-neuro-062012-170304.Sahel J.-A., Roska B. Gene therapy for blindness. Ann. Rev. Neurosci. 2013; 36: 467–488. DOI: 10.1146/annurev-neuro-062012-170304.

The authors declare that there are no conflicts of interest present.