ssmuБюллетень сибирской медициныBulletin of Siberian Medicine1682-03631819-3684Siberian State Medical University, the Ministry of Healthcare of the Russian Federation10.20538/1682-0363-2021-4-32-38ssmu-4577Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL PAPERSВзаимосвязь некоторых параметров мукозального иммунитета полости рта с уровнем витамина D у пациентов с множественным кариесомThe association between parameters of oral mucosal immunity and 25-hydroxyvitamin D in patients with rampant carieshttps://orcid.org/0000-0003-3225-1333ПутневаА. С.PutnevaA. S.

аспирант, кафедра патологической физиологии, 

672090, г. Чита, ул. Горького 39а

39a, Gorkogo Str., Chita, 672090

putnevaas@yandex.ruhttps://orcid.org/0000-0002-0487-6275КараваеваТ. М.KaravaevaT. M.

канд. мед. наук, ст. науч. сотрудник, лаборатория клинической и экспериментальной биохимии и иммунологии, НИИ молекулярной медицины; доцент, кафедра химии и биохимии, 

672090, г. Чита, ул. Горького 39а 

39a, Gorkogo Str., Chita, 672090

https://orcid.org/0000-0002-0487-6275МаксименяМ. В.MaksimenyaM. V.

канд. биол. наук, ст. науч. сотрудник, лаборатория клинической и экспериментальной биохимии и иммунологии, НИИ молекулярной медицины; доцент, кафедра химии и биохимии, 

672090, г. Чита, ул. Горького 39а 

39a, Gorkogo Str., Chita, 672090

https://orcid.org/0000-0002-8601-3499ТерешковП. П.TereshkovP. P.

канд. мед. наук, вед. науч. сотрудник, лаборатория клинической и экспериментальной биохимии и иммунологии, НИИ молекулярной медицины; доцент, кафедра химии и биохимии, 

672090, г. Чита, ул. Горького 39а 

39a, Gorkogo Str., Chita, 672090

https://orcid.org/0000-0003-4678-0527МищенкоМ. Н.MishchenkoM. N.

канд. мед. наук, ассистент, кафедра стоматологии ФПК и ППС,

672090, г. Чита, ул. Горького 39а 

39a, Gorkogo Str., Chita, 672090

https://orcid.org/0000-0002-0724-0352ФефеловаЕ. В.FefelovaE. V.

канд. мед. наук, доцент, кафедра патологической физиологии, 

672090, г. Чита, ул. Горького 39а 

39a, Gorkogo Str., Chita, 672090

https://orcid.org/0000-0002-0975-2351ЦыбиковН. Н.TsybikovN. N.

 д-р мед. наук, профессор, зав. кафедрой патологической физиологии, 

672090, г. Чита, ул. Горького 39а 

39a, Gorkogo Str., Chita, 672090

https://orcid.org/0000-0002-1458-2385ПаршинаА. А.ParshinaA. A.

ассистент, кафедра патологической физиологии, 

672090, г. Чита, ул. Горького 39а 

39a, Gorkogo Str., Chita, 672090

Читинская государственная медицинская академия (ЧГМА)РоссияChita State Medical Academy (CSMA)Russian Federation2021010120222043238Copyright © Путнева А.С., Караваева Т.М., Максименя М.В., Терешков П.П., Мищенко М.Н., Фефелова Е.В., Цыбиков Н.Н., Паршина А.А., 20222022Путнева А.С., Караваева Т.М., Максименя М.В., Терешков П.П., Мищенко М.Н., Фефелова Е.В., Цыбиков Н.Н., Паршина А.А.Putneva A.S., Karavaeva T.M., Maksimenya M.V., Tereshkov P.P., Mishchenko M.N., Fefelova E.V., Tsybikov N.N., Parshina A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://bulletin.ssmu.ru/jour/article/view/4577

Цель – оценить содержание иммунорегуляторных молекул в слюне у лиц с множественным кариесом и дефицитом 25(OH)D3 и определить взаимосвязи их величин с концентрацией 25(OH)D3 в крови.

Материалы и методы

Материалы и методы. Обследованы две группы лиц в возрасте 20–22 лет. В одну включены 15 человек с кариесом и уровнем 25(OH)D3 менее 20 нг/мл, в другую (контрольную) – 15 здоровых человек с содержанием 25(OH)D3 30–100 нг/мл. В ротовой жидкости определены концентрации растворимых форм молекул B7.2 (CD86), Free Active TGF-b1, CTLA-4, PD-1, Tim-3, LAG-3, IGFBP-4, ICAM-1 методом проточной цитофлуометрии, количество кателицидина LL-37, секреторного иммуноглобулина A (IgA) методом иммуноферментного анализа. Между определяемыми показателями рассчитан критерий корреляции Спирмена.

Результаты

Результаты. У лиц с кариесом и дефицитом витамина D выявлено снижение значений Free Active TGF-b1, B7.2 (CD86), PD-1, Tim-3, sIgA, кателицидина LL-37 и повышение уровня IGFBP-4 и ICAM-1 в слюне. Обнаружено наличие прямых корреляционных связей между количеством 25(OH)D3 в крови, с одной стороны, и значениями Free Active TGF-b1, CTLA-4, В7.2 (CD86), секреторного IgA, пептида LL-37 – с другой. Зафиксирована отрицательная взаимосвязь между величинами 25(OH)D3 и ICAM-1.

Заключение

Заключение. На фоне дефицита витамина D при множественном кариесе в ротовой жидкости регистрируются низкие концентрации Free Active TGF-b1, B7.2 (CD86), PD-1, Tim-3, секреторного IgA, кателицидина LL-37 по сравнению с контролем, но увеличены значения IGFBP-4 и ICAM-1. 

Aim

Aim. To determine the saliva level of immunoregulatory proteins in patients with rampant caries and 25-hydroxyvitamin D (25(OH)D) deficiency and evaluate the association of their concentration with 25(OH)D plasma level.

Materials and methods

Materials and methods. The study was performed in two groups. The experimental group included 15 patients aged 20–22 years with rampant caries and the 25(OH)D plasma level of < 20 ng / ml. The control group encompassed 15 healthy age-matched volunteers with the 25(OH)D plasma level of 20–100 ng / ml. The concentrations of B7.2 (CD86), free active TGF-β1, CTLA-4, PD-1, Tim-3, LAG-3, IGFBP-4, and ICAM-1 were assessed using flow cytometry. The levels of LL-37 and secretory immunoglobulin A (sIgA) were measured using ELISA. The Spearman’s rank correlation coefficient was used to reveal a correlation between the indicated proteins and the 25(OH)D plasma level.

Results

Results. A decrease in B7.2 (CD86), PD-1, Tim-3, sIgA, and LL-37 and elevation of IGFBP-4 and ICAM-1 saliva levels were detected in patients with rampant caries and 25-hydroxyvitamin D deficiency. A positive Spearman’s rank correlation coefficient was revealed between plasma 25(OH)D and saliva levels of free active TGF-β1, CTLA4, B7.2 (CD86), LL-37, and sIgA. A negative correlation was revealed between 25(OH)Dand ICAM-1.

Conclusion

Conclusion. 25(OH)D deficiency in patients with rampant caries is associated with decreased levels of B7.2 (CD86), PD-1, Tim-3, sIgA, and LL-37 and elevated levels of IGFBP-4 and ICAM-1 in the saliva. 

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The authors declare that there are no conflicts of interest present.