ssmuБюллетень сибирской медициныBulletin of Siberian Medicine1682-03631819-3684Siberian State Medical University, the Ministry of Healthcare of the Russian Federation10.20538/1682-0363-2024-4-145-157ssmu-5882Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL PAPERSИспользование трехмерной биопечати для регенерации кожи и заживления ран (обзор литературы)The use of three-dimensional bioprinting for skin regeneration and wound healing (literature review)https://orcid.org/0000-0002-3728-9966БарсукИ. А.BarsukI. A.

Барсук Илья Александрович – адъюнкт при научно-исследовательском отделе (медико-биологических исследований) научно-исследовательского центра,

194044, г. Санкт-Петербург, ул. Академика Лебедева, 6

6, Akademika Lebedeva Str., Saint Petersburg, 194044

barsuk20220@gmail.comhttps://orcid.org/0000-0002-1584-1748ГоловкоК. П.GolovkoK. P.

Головко Константин Петрович – д-р мед. наук, доцент, начальник научно-исследовательского центра, 194044, г. Санкт-Петербург, ул. Академика Лебедева, 6;

199034, г. Санкт-Петербург, Университетская наб., 7/9

6, Akademika Lebedeva Str., Saint Petersburg, 194044;

7–9, Universitetskaya Embankment, Saint Petersburg, 199034

labws@mail.ruhttps://orcid.org/0009-0001-9229-5293АлександровВ. Н.AlexandrovV. N.

Александров Виктор Николаевич – д-р мед. наук, профессор, начальник научно-исследовательской лаборатории (тканевой инженерии) научно-исследовательского отдела (медико-биологических исследований) научно-исследовательского центра,

194044, г. Санкт-Петербург, ул. Академика Лебедева, 6

6, Akademika Lebedeva Str., Saint Petersburg, 194044;

2, Litovskaya Str., Saint Petersburg, 194100

vnaleks9@yandex.ruhttps://orcid.org/0009-0003-0763-7194ХасановА. Р.KhasanovA. R.

Хасанов Артур Ришатович – адъюнкт при научно-исследовательском отделе (медико-биологических исследований) научно-исследовательского центра, 194044, г. Санкт-Петербург, ул. Академика Лебедева, 6;

194100, г. Санкт-Петербург, ул. Литовская, 2

6, Akademika Lebedeva Str., Saint Petersburg, 194044

khasartrish@yandex.ruhttps://orcid.org/0009-0006-4989-2523ЭдгеевН. И.EdgeevN. I.

Эдгеев Наран Игоревич – начальник хирургического отделения,

184310, г. Заозерск, ул. Матроса Рябинина, 22

22, Matrosa Ryabinina Str., Murmansk region, Zaozersk, 184310

luxomjachok@mail.ruhttps://orcid.org/0009-0008-6079-956XГалиуллинР. И.GaliullinR. I.

Галиуллин Ринат Ильмирович – старший ординатор хирургического отделения,

184310, г. Заозерск, ул. Матроса Рябинина, 22

22, Matrosa Ryabinina Str., Murmansk region, Zaozersk, 184310

rinat061989@list.ruВоенно-медицинская академия (ВМедА) им. С.М. КироваРоссияS.M. Kirov Military Medical AcademyRussian FederationВоенно-медицинская академия (ВМедА) им. С.М. Кирова; Санкт-Петербургский государственный университет (СПбГУ)РоссияS.M. Kirov Military Medical Academy; Saint Petersburg State UniversityRussian FederationВоенно-медицинская академия (ВМедА) им. С.М. КироваРоссияS.M. Kirov Military Medical Academy; Saint Petersburg State Pediatric Medical UniversityRussian FederationВоенно-медицинская академия (ВМедА) им. С.М. Кирова; Санкт-Петербургский государственный педиатрический медицинский университет (СПбГПМУ)РоссияS.M. Kirov Military Medical AcademyRussian FederationФилиал № 4 1469-го военно-морского клинического госпиталяРоссияBranch No. 4 of Naval Clinical Hospital No. 1469Russian Federation202426012025234145157Copyright © Барсук И.А., Головко К.П., Александров В.Н., Хасанов А.Р., Эдгеев Н.И., Галиуллин Р.И., 20252025Барсук И.А., Головко К.П., Александров В.Н., Хасанов А.Р., Эдгеев Н.И., Галиуллин Р.И.Barsuk I.A., Golovko K.P., Alexandrov V.N., Khasanov A.R., Edgeev N.I., Galiullin R.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://bulletin.ssmu.ru/jour/article/view/5882

Трехмерная биопечать в настоящее время применяется в самых разных областях медицины, являясь движущей силой многих медицинских исследований. Эти исследования способствуют продвижению в область персонализированной медицины, включающих печать сложных человеческих органов для их использования в клинической практике. Одним из ведущих направлений в продвижении трехмерной биопечати является разработка биочернил определенного состава с заданными свойствами.

Цель настоящего систематического обзора состоит в анализе данных современных биомедицинских исследований, касающихся оценки эффективности использования трехмерной биопечати для регенерации кожи и заживления ран. Всеобъемлющий поиск всех релевантных оригинальных статей выполнили на основе заранее определенных критериев приемлемости. Поиск проводили с использованием платформ PubMed, Web of Science, Scopus, Medline Ovid и ScienceDirect.

В результате сужения области поиска из 2 256 статей отобрали 18, полностью соответствовавших критериям включения. Во все отобранные исследования было включено 159 животных с раневыми дефектами. В качестве биочернил чаще всего использовали коллагеновые и желатиновые гидрогели. В части клеточного компонента превалировали аллогенные фибробласты и кератиноциты. Период наблюдения колебался от 1 сут до 6 нед. В большинстве включенных исследований на животных полное закрытие раны достигалось через 2–4 нед.

Результаты как in vitro, так и in vivo показали положительное влияние напечатанных биоинженерных конструкций на ускорение заживления ран. Особый интерес представляет исследование, где биопечать выполняется непосредственно в ране in situ.

Проведенное исследование позволяет сделать вывод о необходимости отработки технологии биопечати тканей для лечения кожных ран на животных моделях с использованием стандартизированных параметров, чтобы открыть двери для клинических испытаний на людях.

Three-dimensional (3D) bioprinting is rapidly proliferating across many medical disciplines and is making strides towards manufacturing intricate human organs for clinical application. One of the most promising areas in 3D bioprinting is development of bioinks with certain composition and designed properties.

The aim of this systematic review was to assess current biomedical research evidence regarding the efficacy of 3D bioprinting for skin regeneration and wound healing. A comprehensive search for all applicable original articles was conducted according to pre-established eligibility criteria. The study employed PubMed, Web of Science, Scopus, Medline Ovid, and ScienceDirect databases.

Of the retrieved articles, eighteen satisfied the inclusion criteria, while twenty-three were excluded. A total of 159 animals that had wound defects were considered in all animal-based research. Collagen and gelatin hydrogels were the most commonly employed bioinks. In relation to cellular composition, allogeneic fibroblasts and keratinocytes were predominant. The observation period ranged from one day to six weeks. Complete wound closure was achieved within 2–4 weeks in most animal studies. In vitro and in vivo animal studies have shown a positive effect of printed bioengineered constructs in accelerating wound healing. Notably, the research where bioprinting was performed directly in the wound in situ was of particular interest. Further studies are required to enhance the tissue bioprinting technique to address skin wound healing in animal models. The utilization of standardized parameters may pave the way for human clinical studies.

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The authors declare that there are no conflicts of interest present.