Morphological and functional state of the epithelial cells in submandibular salivary glands after repeated amputations of the incisor teeth in adult rats
https://doi.org/10.20538/1682-0363-2018-1-53-58
Abstract
Purpose. To evaluate a morphofunctional state of the epithelial cells of the acini and ducts of submandibular glands of mature rats after repeated incisors amputation.
Materials and methods. The experiment was performed on mature (2 months) white male rats, divided into groups: intact, control group and a group of rats subjected to repeated incisors amputation. Applying histological, histochemical and morphometric methods, we evaluated the morphological and functional state of the epithelial cells in submandibular glands of adult rats at 2, 3, 4, 6, 8, 10 and 12 weeks after repeated amputation of the incisor teeth.
Results. There is an increase of submandibular gland acini area at the 3–10 week after repeated incisors amputation. The functional activity of the submandibular glands ducts and acini cells decreases at the 2–4 week of the experiment.
Conclusion. As a result of repeated incisors amputation in rat submandibular glands acini and ducts epithelial cells develop reversible structural and functional changes, which are leveled by the 12th and 6th week of the experiment, respectively.
About the Authors
V. V. Ivanova
Siberian State Medical University.
Russian Federation
Ivanova Vera V., Postgraduate Student, Department of Morphology and General Pathology. 2, Moskow Tract, Tomsk, 634050.
Russian Federation
Ivanova Vera V., Postgraduate Student, Department of Morphology and General Pathology. 2, Moskow Tract, Tomsk, 634050.
I. V. Milto
Siberian State Medical University; National Research Tomsk Polytechnic University.
Russian Federation
Russian Federation
Milto Ivan V., DBSc, Associate Professor, Department of Morphology and General Pathology; Associate Professor, Department of Biotechnology and Organic Chemistry.
2, Moskow Tract, Tomsk, 634050; 30, Lenin Av., Tomsk, 634050.
I. V. Sukhodolo
Siberian State Medical University.
Russian Federation
Russian Federation
Sukhodolo Irina V., DM, Professor, Head of the Department of Morphology and General Pathology.
2, Moskow Tract, Tomsk, 634050.
References
1. Thulesen J., Bor M.V., Thulesen S., Nexо E. et al. Altered secretion and processing of epidermal growth factor in adrenergic-induced growth of the rat submandibular gland. Regulatory Peptides. 2002; 106: 105–114.
2. Adthapanyawanich K., Kumchantuek T., Nakata H., Yamamoto M. et al. Morphology and gene expression profile of the submandibular gland оf androgen-receptor-deficient mice. Arch. Oral. Biol. 2015; 60 (2): 320–332.
3. Amano O., Iseki S. Cell growth factors in salivary glands. Microscope. 2005; 40: 1–6.
4. Takeda Y., Hirose H., Enomoto S. Enlargement of rat submandibular salivary gland induced by single amputation of lower incisor teeth. Histological, histometric and ultrastructural studies. J. Oral Pathol. 1986; 15 (6): 327–333.
5. Almeida de P.V., Grеgio A.M., Machado M.А., de Li- ma A.A. et al. Saliva composition and functions: a comprehensive review. J. Contemp. Dent. Pract. 2008; 9 (3): 72–80.
6. Babayeva А.G., Shubnikova E.А. Structure, function and adaptive growth of the salivary glands. M.: MGU Publ., 1979: 192 (in Russ.).
7. Avtandilov G.G. Medical morphometry (guidance). M.: Meditsina Publ., 1990: 384 (in Russ.).
8. Handelman C.S., Wells H. Morphological and histochemical studies of experimentally enlarged and atrophied salivary glands of rats. Am. J. Anat. 1963; 112: 65–79.
9. Kofoed J.A., Curbelo H.M., Houssay A.B. Acid glycosaminoglycans and sialic acid content in salivary glands of rats. J. Dent. Res. 1969; 48 (4): 555–558.
10. Mehansho H., Carlson D.M. Induction of protein and glycoprotein synthesis in rat submandibular glands by isoproterenol. J. Biol. Chem. 1983; 25: 8 (10): 6616–6620.
11. Wu A.M., Csako G., Herp A. Structure, biosynthesis, and function of salivary mucins. Mol. Cell. Biochem. 1994; 137 (1): 39–55.
Review
For citations:
Ivanova V.V., Milto I.V., Sukhodolo I.V. Morphological and functional state of the epithelial cells in submandibular salivary glands after repeated amputations of the incisor teeth in adult rats. Bulletin of Siberian Medicine. 2018;17(1):53-58. (In Russ.) https://doi.org/10.20538/1682-0363-2018-1-53-58
Views:
961
Email this article 