NEUROTROPIC AND DIURETIC ACTIVITY OF THICK EXTRACT OF THE BLOOD-RED HAWTHORN’S FRUITS

Blood-red hawthorn (Crataegi fructus) are a popular cardiotonic herbal materials, which widely used in medical practice in our country and abroad. In order to prevent diseases of the cardiovascular system is appropriate to prescribe herbal medicines, because they combine the breadth and softness of therapeutic action, along with the absence of a significant number of side effects and contraindications.
The purpose was to study diuretic and antidepressant activity of thick fruit extract of blood-red hawthorn.
Material and methods. Under laboratory conditions we obtained a thick extract of blood-red hawthorn fruits. This phytopharmaceutical was studied by us for any diuretic and antidepressant activity. Investiga- tions were carried out on white rats of both sexes weighing 200–220 g. Each experimental group consist- ed of ten animals. Study drug was administered intragastrically by stomach tube. Control in both cases served as purified water. For determination of diuretic activity, we used a thick extract of hawthorn fruits in a dose of 20 mg/kg against the background of the water load in the amount of 3% of body weight of the animal. Reference preparation was chosen dichlorthiazid 20 mg/kg. It was administered once. After ad- ministration the animals were placed in metabolic cages for urine collection for 24 hours. The study de- termined diuresis, natriuresis, kaliuresis, and kreatininuresis for 4 h and 24 h of the experiment. On the day of the experiment the animals of the control group was administered water load of 3%, and experi- enced – intragastrically drug in an identical amount. Animals were placed in a metabolic cage for a day. Collected 4 h and 24-h urine sample. Determined by renal excretion of water was recorded concentration of sodium and potassium by flame photometry in the fiery liquid analyzer PAG-1, creatinine – colorimet- ric method for photocolorimeter CFC-3. For determining antidepressant activity we used a thick hawthorn extract in a dose of 25 mg/kg of animal body weight. As a synthetic drug comparisons, we used amitriptilin 5 mg/g.
Results. It was found that the thick extract of fruits of blood red hawthorn in a dose of 20 mg/kg for 24 hours increases the diuresis experiment, both by increasing the glomerular filtration rate and by lowering tubular reabsorption. For thick extract of fruits of hawthorn blood-red characteristic expression antide- pressant activity.
Conclusion. The studied preparation of blood-red hawthorn’s fruits have properties to provide complex nephrotropic and neurotropic activity on the living organism.

1. Gosudarstvennaya farmakopeya SSSR. 11-e izd. MZ SSSR. Vyp. 2. Moskow, Meditsina Publ., 1990. 400 p. (in Russian).

2. Kurkin V.A. Farmakognoziya [Pharmacognosy]. Samara, Ofort Publ., SamGMU Publ., 2007. 1239 p. (in Russian).

3. Murav'eva D.A., Samylina I.A., Yakovlev G.P. Farmakognoziya [Pharmacognosy]. Moskow, Medicina Publ., 2002. 656 p. (in Russian).

4. Pastushenkov L.V., Pastushenkov A.L., Pastushenkov V.L. Lekarstvennye rasteniya: Ispol'zovanie v narodnoy meditsine i bytu [Medicinal plants: use in folk medicine and daily life]. Leningrad, Lenizdat Publ., 1990. 284 p. (in Russian).

5. Kurkin V.A., Zaytseva Ye.N., Kurkina A.V., Pravdivtseva O.Ye., Morozova T.V., Garaeva R.R. Diureticheskie svoystva eks- trakta boyaryshnika krovavo-krasnogo [Diuretic properties of the extract of hawthorn blood-red]. Materialy konferentsii “Farmatsiya i obshchestvennoe zdorov'e” [Proceedings of the conference "Pharmacy and Public Health"]. Ekaterinburg, 2014. P. 96–100 (in Russian).

6. Zaytseva Ye.N., Kurkin V.A., Dubishchev A.V., Pravdiv- tseva O.Ye., Zimina L.N. Preparaty na osnove travy zveroboya kak sredstva korrektsii ekskretornoy funktsii pochek [Prepara- tions based on St. John's wort herb as a correction drug of renal excretory function]. Izvestiya Samarskogo nauchnogo tsentra RAN, 2011, vol. 13, no. 1 (8), pp. 1999–2002 (in Russian).

7. Kurkin V.A., Pravdivtseva O.Ye., Zimina L.N. Antidepressantnaya aktivnost' preparatov travy zveroboya [The antidepressant activity of phytopharmaceuticals of St. John's wort]. Farmatsiya, 2010, no. 5, pp. 40–41 (in Rus- sian).

8. Kurkina A.V. Novye podxody k standartizatsii tsvetkov boyaryshnika [New approaches of standardization of hawthorn flowers] // Khimiya rastitel'nogo syr'ya, 2013, no. 2, pp. 171–176 (in Russian).

9. Kurkina A.V. Flavonoidy farmakopeynykh rasteniy [Flavonoids of pharmacopoeia medicinal plants]. Samara, Ofort Publ., SamGMU Publ., 2012, 290 p. (in Russian).

10. Zaytseva Ye.N., Zaytsev A.R., Dubishchev A.V. Patent for an utility model RUS 115651. № 2011138631/13; zayavl. 20.09.11; opubl. 10.05.12, Byul. no. 13, 2 p. (in Russian).

11. Berkhin Ye.B. Metody eksperimental'nogo issledovaniya pochek i vodno-solevogo obmena [Methods of experimental studies of the kidneys and water-salt metabolism]. Barnaul, Omskaya pravda Publ., 1972. 200 p. (in Russian).

12. Zaytseva Ye.N. Patent for an invention RUS 2494703. № 2012104057/13; zayavl. 06.02.12; opubl. 10.10.13, Byul. no. 28, 11 p. (in Russian).

13. Xabriev R.U. Manual on experimental (preclinical) study of new pharmacological substances. 2nd Edition. Ed. R.U. Hab- riev. Moscow, Medicine Publ., 2005. 832 p. (in Russian).