Study of the IL-4 and IL-4δ2 interaction with the IL-4R receptors of the I and II types in silico and the effect on the functional activity of mononuclear cells in vitro
https://doi.org/10.20538/1682-0363-2010-3-51-56
Abstract
Alternatively spliced interleukins are very actively studied over the last years. Splice form of IL-4 — IL-4δ2 has antagonistic effects to its full form on proliferative activity of human mononuclear blood cells and their IL-6 production. Antagonistic effects between IL-4 and IL-4δ2 were confirmed and explained in this study due to combination of biochemical and computer methods.
About the Authors
K. V. Goremykin
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
A. N. Silkov
НИИ клинической иммунологии СО РАН
Russian Federation
Russian Federation
B. V. Shilov
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
V. Yu. Serebrov
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
A. E. Sazonov
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
S. V. Sennikov
НИИ клинической иммунологии СО РАН
Russian Federation
Russian Federation
References
1. Птицын Л.Р., Смирнов С.В., Альтман И.Б. и др. Продукция рекомбинантного hIL-4δ2 клетками Escherichia coli//Биоорган. химия. 1999. Т. 25. С. 623-629.
2. Степанов Н.Ф., Пупышев В.И. Квантовая механика молекул и квантовая химия. М.: Изд-во МГУ, 1991. 384 с.
3. Alms W.J., Atamas S.P., Yurovsky V.V. et al. Generation of a variant of human interleukin-4 by alternative splicing//Mol. Immunol. 1996. V. 33. P. 361-370.
4. Arinobu Y., Atamas S.P., Otsuka T. et al. Antagonistic effects of an alternative splice variant of human IL-4, IL-4delta2, on IL-4 activities in human monocytes and B cells//Cell. Immunol. 1999. V. 191. P. 161-167.
5. Atamas S.P. Alternative splice variants of cytokines: making a list//Life Sci. 1997. V. 61. P. 1105-1112.
6. Atamas S.P., Choi J., Yurovsky V.V. et al. An alternative splice variant of human IL-4, IL-4 delta 2, inhibits IL-4-stimulated T cell proliferation//J. Immunol. 1996. V. 156. P. 435-441.
7. Atamas S.P., White B. Interleukin 4 in systemic sclerosis: not just an increase//Clin. Diagn. Lab. Immunol. 1999. V. 6. P. 658-659.
8. Barrera P., Faure S., Prud'homme J.F. et al. European genetic study on rheumatoid arthritis: is there a linkage of the interleukin-1 (IL-1), IL-10 or IL-4 genes to RA?//Clin. Exp. Rheumatol. 2001. V. 19. P. 709-714.
9. Berman H.M., Westbrook J., Feng Z. et al. The Protein Data Bank//Nucleic Acids Res. 2000. V. 28. № 1. P. 235-242.
10. Callard R.E., Matthews D.J., Hibbert L. IL-4 and IL-13 receptors: are they one and the same?//Immunol. Today. 1996. V. 17. P. 108-110.
11. Chomarat P., Banchereau J. An update on interleukin-4 and its receptor//Eur. Cytokine Netw. 1997. V. 8. P. 333-344.
12. Isakson P.C., Pure E., Vitetta E.S. et al. T cell-derived B cell differentiation factor(s). Effect on the isotype switch of murine B cells//J. Exp. Med. 1982. V. 155. P. 734-748.
13. Klein S.C., Golverdingen J.G., Bouwens A.G. et al. An alternatively spliced interleukin 4 form in lymphoid cells//Immunogenetics. 1995. V. 41. P. 57.
14. Klein S.C., Golverdingen J.G., van Wichen D.F. et al. Expression of two interleukin 4 mRNA isoforms in B lymphoid cells//Cell. Immunol. 1996. V. 167. P. 259-268.
15. Morris G.M., Huey R., Olson A.J. Using AutoDock for ligand-receptor docking//Curr Protoc Bioinformatics. 2008. V. 11, № 3. P. 34-37.
16. Russell S.M., Keegan A.D., Harada N. et al. Interleukin-2 receptor gamma chain: a functional component of the interleukin-4 receptor//Science. 1993. V. 262. P. 1880-1883.
17. Seah G.T., Gao P.S., Hopkin J.M. et al. Interleukin-4 and its alternatively spliced variant (IL-4 delta 2) in patients with atopic asthma//Am. J. Respir. Crit. Care Med. 2001. V. 164. P. 1016-1018.
18. Seah G.T., Rook G.A. High levels of mRNA encoding IL-4 in unstimulated peripheral blood mononuclear cells from tuberculosis patients revealed by quantitative nested reverse transcriptase-polyme rase chain reaction; correlations with serum IgE levels//Scand. J. Infect. Dis. 2001. V. 33. P. 106-109.
19. Sennikov S.V., Krysov S.V., Injelevskaya T.V. et al. Quantitative analysis of human immunoregulatory cytokines by electrochemiluminescence method//J. Immunol. Methods. 2003. V. 275. P. 81-88.
20. Zav'yalov V.P., Denesyuk A.I., White B. et al. Molecular model of an alternative splice variant of human IL-4, IL-4 delta 2, a naturally occurring inhibitor of IL-4-stimulated T cell proliferation//Immunol lett. 1997. V. 58. P. 149-152. 1.
Review
For citations:
Goremykin K.V., Silkov A.N., Shilov B.V., Serebrov V.Yu., Sazonov A.E., Sennikov S.V. Study of the IL-4 and IL-4δ2 interaction with the IL-4R receptors of the I and II types in silico and the effect on the functional activity of mononuclear cells in vitro. Bulletin of Siberian Medicine. 2010;9(3):51-56. (In Russ.) https://doi.org/10.20538/1682-0363-2010-3-51-56
Views:
502
Email this article 