Action of inhibitors Heat shock proteins 90 and 27 on dexamethasone-induced apoptosis of tumor cells
https://doi.org/10.20538/1682-0363-2010-3-68-71
Abstract
Programmed cell death of tumor cells of line Jurkat in conditions of cultivation with various concentration of dexamethasone, selective inhibitors of Hsp90 (Heat shock protein — Hsp) (17-AAG) and Hsp27 (KRIBB3) was investigated. An estimation of realisation apoptosis spent by method of fluorescent microscopy with use annexin V-FITC and propidium iodide. Inhibition of Hsp90 and Hsp27 leads to activation of tumor cells Jurkat apoptotic program and strengthening of dexamethasone-induced apoptosis. Hsp27 and Hsр90 play an antiapoptotic role in tumor cells of line Jurkat.
About the Authors
Ye. V. Kaigorodova
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
N. V. Ryazantseva
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
V. V. Novitsky
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
A. N. Maroshkina
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
M. V. Belkina
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
O. Ye. Chechina
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
A. P. Zima
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
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Review
For citations:
Kaigorodova Ye.V., Ryazantseva N.V., Novitsky V.V., Maroshkina A.N., Belkina M.V., Chechina O.Ye., Zima A.P. Action of inhibitors Heat shock proteins 90 and 27 on dexamethasone-induced apoptosis of tumor cells. Bulletin of Siberian Medicine. 2010;9(3):68-71. (In Russ.) https://doi.org/10.20538/1682-0363-2010-3-68-71
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