Hydrogen peroxide influence on contractile activity smooth muscle cells: the role of cytoskeleton
https://doi.org/10.20538/1682-0363-2009-4-41-46
Abstract
The influence of of hydrogen peroxide on the contractile reactions of smooth muscle cells caused by hyperpotassium solution end phenylephrine in modulation a potassium conductance the membrane and the state of cytoskeleton elements has been investigated by the mechanographical method. It has multidirectional influence of hydrogen peroxide in the reduction of smooth muscles of rat aorta with the membrane depolarization hyperpotassium solution and action phenylephrine: phenylephrine decline in value and increase strength hyperpotassium contractures. We show that the cytoskeleton components involved in the mechanisms of action of hydrogen peroxide in the contractile reactions of smooth muscles of rat aorta caused by phenylephrine.
About the Authors
I. V. Kovalyev
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
S. V. Gusakova
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
O. S. Melnik
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
M. B. Baskakov
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
L. V. Kapilevich
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
M. A. Medvedev
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
V. B. Studnitsky
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
O. I. Antonov
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
References
1. Баскаков М.Б., Медведев М.А., Ковалёв И.В. и др. Механизмы регуляции функций гладких мышц вторичными посредниками. Томск: Гавань, 1996. 154 с.
2. Шуба М.Ф., Кочемасова Н.Г. Физиология сосудистых гладких мышц. Киев: Наукова думка, 1988. 250 с.
3. Barlow R.S., El Mowafy A.M., White R.E. H2O2 opens BKCa channels via the PLA2-arachidonic acid signaling cascade in coronary artery smooth muscle//Am. J. Physiol. Heart Circ. Physiol. 2000. V. 279. P. 475-483.
4. Dalle-Donne I., Rossi R., Milzani A. et al. The actin cytoskeleton response to oxidants: from small heat shock protein phosphorylation to changes in the redox state of actin itself//Free radical biology & medicine. 2001. № 31 (12). P. 1624-1632.
5. Dröge W. Free Radicals in the Physiological Control of Cell Function//Physiological Reviews. 2002. V. 82, № 1. P. 47-95.
6. Esteve J.M., Mompo J., Garcia de Laasuncioon J., Sastre J. Oxidative damage to mitochondrial DNA and glutathione oxidation in apoptosis: studies in vivo and in vitro//The FASEB Journal. 1999. № 13. P. 1055-1064.
7. Rogers A.M. Rapid vasoregulatory mechanisms in exercising human skeletal muscle: dynamic response to repeated changes in contraction intensity//Am. J. Physiol. Heart Circ. Physiol. 2006. V. 10. P. 355-368.
8. Thakali K., Demel S.L., Fink G.D., Watts S.W. Endothelin-1-induced contraction in veins is independent of hydrogen peroxide//Am. J. Physiol. Heart Circ. Physiol. 2005. V. 289. P. 1115-1122.
9. Touyz R.M., Yao G., Quinn M.T. et al. p47phox Associates With the Cytoskeleton Through Cortactin in Human Vascular Smooth Muscle Cells Role in NAD(P)H Oxidase Regulation by Angiotensin II//Arteriosclerosis, Thrombosis, and Vascular Biology. 2005. V. 25. P. 512-518.
10. Ursini F., Maiorino M., Brigelius-Flohe´ R. et al. Roveri Diversity of glutathione peroxidases//Methods Enzymol. 1995. V. 252. P. 38-53.
11. Valen G., Sonden A., Vaage J. Hydrogen peroxide induces endothelial cell atypia and cytoskeleton depolymerization//Free Radical Biology and Medicine. 1999. V. 26, № 11. P. 1480-1488.
12. Yang Z., Zheng T., Zhang A. et al. Mechanisms of hydrogen peroxide-induced contraction of rat aorta//Eur. J. Pharmacol. 1998. V. 344. P. 169-181.
13. Zhao Y., Davis H.W. Hydrogen peroxide-induced cytoskeletal rearrangement in cultured pulmonary endothelial cells//J. Cell. Physiol. 1998. V.174. P.370-379.
14. Zhu D., Tan K.S., Zhang X. et al. Hydrogen peroxide alters membrane and cytoskeleton properties and increases intercellular connections in astrocytes//J. of Cell Sci. 2005. V. 188. P. 3695-3703.
15. Zuo L., Ushio-Fukai M., Hilenski L.L., Wayne Alexander R. Microtubules Regulate Angiotensin II Type 1 Receptor and Rac1 Localization in Caveolae/Lipid Rafts Role in Redox Signaling//Arterioscler. Thromb. Vasc. Biol. 2004. V. 24. P. 1223-1228.
Review
For citations:
Kovalyev I.V., Gusakova S.V., Melnik O.S., Baskakov M.B., Kapilevich L.V., Medvedev M.A., Studnitsky V.B., Antonov O.I. Hydrogen peroxide influence on contractile activity smooth muscle cells: the role of cytoskeleton. Bulletin of Siberian Medicine. 2009;8(4):41-46. (In Russ.) https://doi.org/10.20538/1682-0363-2009-4-41-46
Views:
1143
Email this article 