EFFECT OF OXIDATIVE STRESS ON HISTOARCHITECTONICS AND ELEMENTAL COMPOSITION OF BONE TISSUE OF THE VERTEBRAL BODY RATS

This paper is concerned with study an influence of the oxidative stress on morphofunctional characteris-tics and elemental composition of bone tissue of the vertebral body rats.

Material and methods. The research carried out on male rats of the Wistar line with weight 250–300 g. All rats were categorized into 4 groups with 10 ones in each: 1 – intact group; 2 and 3 – experimental groups; 4 – control group. Rats from groups 2–4 were under the development of oxidized stress which caused by daily stomach-pump injection (during fortnight) with aqua suspension of synthetic glucocorticoid “Prednisolone” in dose 50 mg/kg. Through three hours after “Prednisolone” the antioxidant “Tiophan” was injected by stomach-pump (dose 100 mg/kg), which was dissolved in 0.2 ml of vegetation oil to laboratory animals from the group 2. Rats from control group got only solvent (vegetative oil 0.2 ml) according the same scheme. Three hours after “Prednisolone” to rats from group 3 were injected of 0.2 ml aquaFontana. It was made for purity the experiment and standartization of manipulations, which are connected with injections into rats’ organisms. The peculiarities of structure of bone tissue of vertebra have been studied by methods of morphohystochemical analysis. The elemental composition of bone tissue was defined by atom-emission analysis method with inductive coupled plasma (spectrometer OPTIMA, code of methodics KHA: MUK.4.1.1482-03).

Results. A development of glucocorticoid-induced oxidative stress results to infraction the histoarchitectonics of bone tissue of vertebra and decreasing the content of cations, which are included to the composition of active centres of pherments of antioxidant protection and prosthetic groups of proteins with an antioxidant activity. Using the antioxidant “Tiophan” proved an existence of its expressed osteoprotective properties. This can be seen from decreasing a level of resorbtion of bone tissue of vertebra while modeling the oxidative stress and increasing the content of Ca, P, Cu, Fe, Zn in bone tissue in compare to data of intact animals.

Conclusion. Polyfunctional S-containing antioxidant of new generation “Tiophan” is a prospective remedy of protecting cells and matrix of bone tissue against infraction caused by active oxygen metabolites during an oxidative stress. 

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