CHANGES IN THE GLUTATHIONE SYSTEM IN P19 EMBRYONAL CARCINOMA CELLS UNDER HYPOXIC CONDITIONS
https://doi.org/10.20538/1682-0363-2015-4-41-45
Abstract
Introduction. According to modern perceptions, tumor growth, along with oxidative stress formation, is accompanied by hypoxia. Nowadays studying the regulation of cellular molecular system functioning by conformational changes in proteins appears to be a topical issue. Research goal was to evaluate the state of the glutathione system and the level of protein glutathionylation in P19 embryonal carcinoma (EC) cells under hypoxic conditions.
Material and methods. P19 EC cells (mouse embryonal carcinoma) cultured under normoxic and hypox-ic conditions served the research material.The concentration of total, oxidized, reduced and protein-bound glutathione, the reduced to oxidized thiol ratio as well as glutathione peroxidase and glutathione reductase activity were determined by spectropho-tometry.
Results. Glutathione imbalance was accompanied by a decrease in P19 EC cell redox status under hypox-ic conditions against the backdrop of a rise in protein-bound glutathione.
Conclusions. As a result of the conducted study oxidative stress formation was identified when modeling hypoxia in P19 embryonal carcinoma cells. The rise in the concentration of protein-bound glutathione may indicate the role of protein glutathionylation in regulation of P19 cell metabolism and functions un-der hypoxia.
About the Authors
D. S. Orlov
Siberian State Medical University, Tomsk
Russian Federation
Orlov Dmitry S.
Russian Federation
Orlov Dmitry S.
N. V. Ryazantseva
Siberian Federal University, Krasnoyarsk;
Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky
Russian Federation
Ryazantseva Natalia V.
Russian Federation
Ryazantseva Natalia V.
Ye. A. Stepovaya
Siberian State Medical University, Tomsk
Russian Federation
Stepovaya Yelena A.
Russian Federation
Stepovaya Yelena A.
O. L. Nosareva
Siberian State Medical University, Tomsk
Russian Federation
Nosareva Olga L.
Russian Federation
Nosareva Olga L.
Ye. V. Shakhristova
Siberian State Medical University, Tomsk
Russian Federation
Shakhristova Yevgenia V.
Russian Federation
Shakhristova Yevgenia V.
V. V. Ivanov
Siberian State Medical University, Tomsk
Russian Federation
Ivanov Vladimir V.
Russian Federation
Ivanov Vladimir V.
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Review
For citations:
Orlov D.S., Ryazantseva N.V., Stepovaya Ye.A., Nosareva O.L., Shakhristova Ye.V., Ivanov V.V. CHANGES IN THE GLUTATHIONE SYSTEM IN P19 EMBRYONAL CARCINOMA CELLS UNDER HYPOXIC CONDITIONS. Bulletin of Siberian Medicine. 2015;14(4):41-45. (In Russ.) https://doi.org/10.20538/1682-0363-2015-4-41-45
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