Сontent of cytokines and growth factors in the intraocular fluid of patients with primary open-angle glaucoma
V. V. Chernykh,
V. I. Konenkov,
O. V. Ermakova,
N. B. Orlov,
O. O. Obukhova,
A. V. Eremina,
A. N. Trunov https://doi.org/10.20538/1682-0363-2019-1-257-265
Abstract
Purpose. To study the content of cytokines and growth factors in the intraocular fluid of patients with developed stage of primary open-angle glaucoma (POAG).
Materials and methods. 56 patients with a verified diagnosis developed stage of primary open-angle glaucomawere examined. The control group consisted of 30 patients with a diagnosis of uncomplicated cataract. A concentration of 17 cytokinesand 3 isoforms of the transforming growth factor (TGF) β was determined using a Bio-Plex Pro™ Human Cytokine 17-plex Assay and Bio-Plex Pro™ and TGFβ 3-plex Assay kit by flow-through fluorometry on a Bio-Plex 200, Bio-Rad double beam laser analyzer, USA.
Results. Astatistically significant increase was shown in the concentrations of cytokines and growth factors (interleukins (IL) 4, 6, 7, 8, 12, 17, TGFβ 1, 2, 3, macrophage inflammatory protein 1 β) in the intraocular fluid of patients with developed stage of the primary open-angle glaucoma in respect to data obtained from the study of the intraocular fluid of the persons with uncomplicated cataract, as well as a statistically significant decrease in the concentrations IL-2, IL-10, granulocyte-macrophage colony-stimulating factor.
Conclusion. In the pathogenesis of primary open-angle glaucoma, the activity of the local chronic inflammatory process is determined. This is confirmed by statistically significant changes in the studied cytokines and growth factors. Increase in the concentrations of the studied representatives of the superfamily of transforming growth factors-beta, which have anti-inflammatory activity, the ability to stimulate proliferation, cell growth, synthesis of extracellular matrix proteins, etc., attests to their importance in the mechanisms of primary open-angle glaucoma development. Increase concentrations of IL-7 in the intraocular fluid of patients with primary open-angle glaucoma allows us to assume participation in the pathogenesis of the primary open-angle glaucoma of this autocrine mediator of activation of the growth of lymphatic structures.
About the Authors
V. V. Chernykh
The Academician S.N. Fyodorov Federal State Institution National Medical Research Center (NMRC)«Intersectoral Research and Technology Complex «Eye microsurgery» Ministry of Health of the Russian Federation»
Russian Federation
Russian Federation
Chernykh Valeriy V., DM, Professor, Director
Novosibirsk Branch
10, Kolkhidskaya Str., Novosibirsk, 630071
V. I. Konenkov
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center of the Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences (RICEL – Branch of IC&G SB RAS)
Russian Federation
Russian Federation
Konenkov Vladimir I., DM, Professor, Academician of RAS, Head of the Laboratory of Clinical Immunogenetics
2, Timakova Str., Novosibirsk, 630117
O. V. Ermakova
The Academician S.N. Fyodorov Federal State Institution National Medical Research Center (NMRC)«Intersectoral Research and Technology Complex «Eye microsurgery» Ministry of Health of the Russian Federation»
Russian Federation
Russian Federation
Ermakova Olga V., Ophthalmologist
Novosibirsk Branch
10, Kolkhidskaya Str., Novosibirsk, 630071
N. B. Orlov
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center of the Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences (RICEL – Branch of IC&G SB RAS)
Russian Federation
Russian Federation
Orlov Nikolai B., DM, Senior Researcher, Laboratory of Clinical Immunogenetics
2, Timakova Str., Novosibirsk, 630117
O. O. Obukhova
Federal Research Center for Fundamental and Translational Medicine of the Siberian Branch of the Russian Academy of Sciences (FIZ FTM SB RAS)
Russian Federation
Russian Federation
Obukhova Olga O., DM, Head of the Laboratory of Immunology
2, Timakova Str., Novosibirsk, 630117
A. V. Eremina
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center of the Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences (RICEL – Branch of IC&G SB RAS)
Russian Federation
Russian Federation
Eremina Alena V., PhD, Researcher, Novosibirsk Branch of the Academician S.N. Fyodorov Federal State Institution NMRC «Intersectoral Research and Technology Complex «Eye microsurgery» Ministry of Health of the Russian Federation
2, Timakova Str., Novosibirsk, 630117
A. N. Trunov
The Academician S.N. Fyodorov Federal State Institution National Medical Research Center (NMRC)«Intersectoral Research and Technology Complex «Eye microsurgery» Ministry of Health of the Russian Federation»;
Federal Research Center for Fundamental and Translational Medicine of the Siberian Branch of the Russian Academy of Sciences (FIZ FTM SB RAS)
Russian Federation
Russian Federation
Trunov Aleksandr N., DM, Professor, Deputy Director for Science, Novosibirsk Branch of the Academician S.N. Fyodorov Federal State Institution NMRC «Intersectoral Research and Technology Complex «Eye microsurgery» Ministry of Health of the Russian Federation; Chief Researcher, Laboratory of Immunology, FIZ FTM SB RAS
10, Kolkhidskaya Str., Novosibirsk, 630071,
2, Timakova Str., Novosibirsk, 630117
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Chernykh V.V., Konenkov V.I., Ermakova O.V., Orlov N.B., Obukhova O.O., Eremina A.V., Trunov A.N. Сontent of cytokines and growth factors in the intraocular fluid of patients with primary open-angle glaucoma. Bulletin of Siberian Medicine. 2019;18(1):257-265. (In Russ.) https://doi.org/10.20538/1682-0363-2019-1-257-265
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