The influence of humoral factors of homeostatistic proliferation on t-regulatory cells in vitro
https://doi.org/10.20538/1682-0363-2019-1-286-293
Abstract
Aim. The aim of this study was the investigation of the influence of humoral factors of homeostatic proliferation IL-7 and IL-15 on T-regulatory cells in healthy donors.
Materials and methods. The study included 15 conditionally healthy donors. Phenotyping and evaluation of expression changes of transcription factor FoxP3 and the main functional molecules on T-regulatory cells such as PD-L1, CTLA-4 and HLA-DR during cultivation under IL-7, IL-15 and anti-CD3 stimulation combined with IL-2 were performed by flow cytometry. Also, we estimated proliferation intensity of T-regulatory cells in the course of cultivation.
Results. We revealed that humoral factors of homeostatic proliferation can effectively support a pool of T-regulatory cells during cultivation by number and phenotype and can maintain expression of important molecules such as PD-L1 and HLA-DR on regulatory T-cell surface. In addition, our study showed that IL-7 and IL-15 can cause relatively low T-regulatory cells proliferation in comparison to CD4+- lymphocytes.
Conclusion. The observed ability of homeostatic proliferation factors to maintain T-regulatory cells pool presumably can play an important role in lymphopenic conditions when the number of effector cells is decreased and the insufficiency of interleukin IL-2 is observed, which plays a primary role in the homeostasis of T-regulatory cells in normal conditions.
Keywords
T-regulatory cells,
interleukin-7,
interleukin-15,
transcriptional factor FoxP3,
suppression molecules
Supporting agencies: Russian Foundation for Basic Research and the Novosibirsk Region (No. 17-44-540167r_a)
About the Authors
D. V. Shevyrev
Research Institute of Fundamental and Clinical Immunology (RIFCI)
Russian Federation
Russian Federation
Shevyrev Daniil V., PhD Student, Laboratory of Clinical Immunopathology
14, Yadrintsevskaya Str., Novosibirsk, 630099
E. A. Blinova
Research Institute of Fundamental and Clinical Immunology (RIFCI)
Russian Federation
Russian Federation
Blinova Elena A., PhD, Senior Researcher, Laboratory of Clinical Immunopathology
14, Yadrintsevskaya Str., Novosibirsk, 630099
V. A. Kozlov
Research Institute of Fundamental and Clinical Immunology (RIFCI)
Russian Federation
Russian Federation
Kozlov Vladimir A., DM, Professor, Аcademician of RAS, Scientific Supervisor of RIFCI, Head of the Laboratory of Clinical Immunopathology, RIFCI
14, Yadrintsevskaya Str., Novosibirsk, 630099
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Review
For citations:
Shevyrev D.V., Blinova E.A., Kozlov V.A. The influence of humoral factors of homeostatistic proliferation on t-regulatory cells in vitro. Bulletin of Siberian Medicine. 2019;18(1):286-293. (In Russ.) https://doi.org/10.20538/1682-0363-2019-1-286-293
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