Morphology of autolymphocytes used in the treatment of trophic ulcers in the setting of diabetic foot syndrome

Abstract

Diabetic foot syndrome is one of the most often chronic and severe complications of diabetes mellitus. Method of local treat-ment of trophic ulcers and wounds in the setting of diabetic foot syndrome using autolymphocytes processed by leukinoferon was developed in the Institute of Clinical and Experimental Lymphology (Novossibirsk). Autolymphocytes were obtained by lymphocytapheresis on the blood cell separator «Haemonetics MCS+». The study of ultrastructural organization of identified lym-phocytes processed by leukinoferon did not reveal any disturbances of their structural integrity. Numerical density free polysomal ribosomes increased by 50%. Volumetric density of vesicular structures in cytoplasm of lymphocytes increased by 3 times. Number of excrescences and microvilli formed by plasmatic membrane increased by 2,4 times. Thus, technologies of autolymphocytes iden-tification do not result to damaged structure of cells. Processing of the lymphocytes by leukinoferon does not cause cellular damage but promotes development of structural changes.