Features of the apoptosis markers expression by skin cell with ageing
https://doi.org/10.20538/1682-0363-2008-2-23-28
Abstract
The immunohistochemical study of skin was conducted to verify presence of p53, bcl-2 and bax apoptosis markers in epidermal keratinocytes of patients from two age groups: 16 to 44 years old and 45 to 64 years old. It was discovered that with chronological ageing of skin the expression levels of p53 and bax apoptosis markers are raised by epidermal cells in patients of elder age group. Obtained data testifies to cumulative damaging effect of a quantity of proapoptotic factors (oxidative stress, UV-waves, ionizing radiation, chemical reagents etc.) on epidermal cells with ageing.
About the Authors
T. Yu. Vitruk
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
N. V. Ryazantseva
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
P. N. Pesterev
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
L. R. Mustafina
Сибирский государственный медицинский университет
Russian Federation
Russian Federation
References
1. Жижина Г.П. Роль апоптоза в нормальном онтогенезе, патогенезе и старении // Клинич. геронтология. 2002. № 4. С. 3—10.
2. Иванова Е. Келоидные и гипертрофические рубцы: вопросы патогенеза // Косметика и медицина. 2007. № 2. С. 4—11.
3. Семкин В.И. Морфофункциональные изменения эпидермальных дифферонов при старении // Клинич. геронтология. 2001. № 9. С. 27—31.
4. Нейроиммуноэндокринология кожи и молекулярные маркеры ее старения / И.О. Смирнова, И.М. Кветной, И.В. Князькин, С.И. Данилов. СПб.: Деан, 2005. 288 с.
5. Руководство по иммуногистохимической диагностике опухолей человека / Под ред. С.В. Петрова, Н.Т. Райхлина. Казань, 2000.278 с.
6. Эллиниди В.Н., Аникеева Н.В., Максимова Н.А. Практическая иммуногистохимия. СПб.: ВУЭРМ МЧС России, 2000. 36 с.
7. Adams J.M., Cory S. The Bcl-2 protein family: arbiters of cell survival // Science. 1998. V. 281. № 5381. P. 1322—1326.
8. Balajee A.S., May A., Bohr V.A. Fine structural analysis of DNA repair in mammalian cells // Mutat. Res. 1998. V. 404. № 1. P. 3—11.
9. Blatt T., Mundt C., Mummert C. et al. Modulation des oxidatives stresses inder humanen altershaut // Z. Gerontol. Geriatr. 1999. Bd. 32. № 2. S. 83—88.
10. Bosset S., Bonnet-Duquennoy M., Barre P. et al. Decreased expression of keratinocyte betal integrins in chronically sun-exposed skin in vivo // Br. J. Dermatol. 2003. V. 148. № 4. P. 770—778.
11. Bowen A.R., Hanks A.N., Allen S.M. et al. Apoptosis regulators and responses in human melanocytic and keratinocytic cells // J. Invest. Dermatol. 2003. V. 120. № 1. P. 48—55.
12. Chao D.T., Korsmeyer S.J. BCL-2 family: regulators of cell death // Ann. Rev. Immunol. 1998. № 16. P. 395—419.
13. Chodon T., Sugihara T., Igava H.H. et al. Keloid-derived fibroblasts are refractory to fas-mediatet apoptosis and neutralization of autocrine transforming growth factor-beta 1 can abrogate this rezistance // Am. J Pathol. 2000. V. 157. № 5. P. 1661—1669.
14. El-Domiaty M., Attia S., Saleh F. et al. Instrinic ageing vs pho-toaging: a comparative histopathological, immunohistochemical and ultrastractural study of skin // Exp. Dermatol. 2002. V. 11. № 5. P. 398 —405.
15. Engelke M., Jensen J.M., Ekanayake-Mudiyanselage S., Proksch E. Effects of xerosis and ageing on epidermal proliferation and differentiation // Br. J. Dermatol. 1997. V. 137. № 2. P. 219— 225.
16. Fujiwara M., Muragaki Y., Ooshima A. Upregulation of transforming growth factor-beta1 and vascular endothelial growth factor in cultured keloid fibroblasts: relevance to angiogenic activity // Arch. Dermatol. Res. 2005. V. 297. № 4. P. 161—169.
17. Green D.R., Beere H.M. Apoptosis. Gone but not forgotten // Nature. 2000. V. 405. № 6782. P. 28—29.
18. Grewe M. Chronological ageing and photoageing of dendritic cells // Clin. Exp. Dermatol. 2001. V. 26. № 7. P. 608—612.
19. Harley C.B., Sherwood S.W. Telomerase, checkpoints and cancer // Cancer Surv. 1997. № 29. P. 263—284.
20. Hockenbery D.M., Zutter M., Hickey W. et al. BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death // Proc. Natl. Acad. Sci. USA. 1991. V. 88. № 16. P. 6961— 6965.
21. Jost M., Class R., Kari C. et al. A central role of Bcl-X(L) in the regulation of keratinocyte survival by autocrine EGFR ligands // J. Invest. Dermatol. 1999. V. 112. № 4. P. 443—449.
22. Krajewski S., Bodrug S., Krajewska A. et al. Immunohistochemical analysis of mcl-1 protein and bcl-2 protein production suggest a unigue role for mcl-1 in control of programmed cell death in vivo // Am. J. Pathol. 1995. V. 146. № 6. P. 1309—1319.
23. Lane D.P. Worrying about p53 // Curr. Biol. 1992. V. 2. № 11. P. 581 —583.
24. Ling G., Person A., Berne B. et al. Persistent p53 mutations in single cells from normal human skin // Am. J. Pathol. 2001. V. 159. № 4. P. 1247—1253.
25. Lu Q.L., Abel P., Foster C.S., Lalani E.N. Bcl-2: role in epithelial differentiation and oncogenesis // Hum. Pathol. 1996. V. 27. № 2. P. 102—110.
26. Nakagawa K., Yamamura K., Maeda S., Ichihashi. Bcl-2 expression in epidermal keratinocytic diseases // Cancer. 1994. V. 74. № 6. P. 1720—1724.
27. Nunez G., Benedict M.A., Hu Y., Inohara N. Caspases: the proteases of the apoptotic pathway // Oncogene. 1998. V. 17. № 25. P. 3237—3245.
28. Pincelli C., Hakke A.R., Bernassi L. et al. Autocrine nerve growth factor protects human keratinocytes from apoptosis through its high affinity receptor (trk): a role for bcl-2 // J. Invest. Dermatol. 1997. V. 109. № 6. P. 757—764.
29. Ponten F., Berne B., Ren Z.P. et al. Ultraviolet light induces expression of p53 and p21 in human skin: effect of sunscreen and consti tutive p21 expression in skin appendages // J. Invest. Dermatol. 1995. V. 105. № 3. P. 402—406.
30. Qin J.Z., Chaturvedi V., Denning M.F. et al. Regulation of apoptosis by p53 in UV-irradiated human epidermis, psoriatic plaques and senescent keratinocytes // Oncogene. 2002. V. 21. № 19. P. 2991— 3002.
31. Reed J.C. Bcl-2 family proteins // Oncogene. 1998. V. 17. № 25. P. 3225—3236.
32. Stefanato C.M., Yaar M., Bhawan J. et al. Modulations of nerve growth factor and Bcl-2 in ultraviolet-irradiated human epidermis // J. Cutan. Pathol. 2003. V. 30. № 6. P. 351—357.
33. Tao W., Levine A.J. Nucleocytoplasmic shuttling of oncoprotein Hdm2 is required for Hdm2-mediated degradation of p53 // Proc. Natl. Acad. Sci USA. 1999. V. 96. № 6. P. 30077—3080.
34. Wang E. Failure to undergo programmed cell death in senescent human fibroblasts in related to inability to down regulate bcl-2 pres ence // Cancer Res. 1995. № 55. P. 2284—2292.
35. Wang Y., Rosenstein B., Goldwyn S. et al. Differential regulation of P53 and Bcl-2 expression by ultraviolet A and В // Dermatol. 1998. V. 111. № 3. P. 380—384.
36. Wassberg C., Backwall H., Diffey B. et al. Enhanced epidermal ultraviolet responses in chronically sun-exposed skin are dependent on previous exposure // Acta. Derm. Veneorol. 2003. V. 83. № 4. P. 254—261.
37. Yang J., Liu X., Bhalla L. et al. Prevention of apoptosis by Bcl-2: release of cytochrome С from mitochondria blocked // Science. 1997. V. 275. № 5303. P. 1129—1132.
38. Zhai S., Yaar M., Doyle S.M., Gilchrest B.A. Nerve growth |factorrescues pigment cells from ultraviolet-induced apoptosis by up-regulating BCL-2 levels // Exp. Cell. Res. 1996. V. 1. № 2. P. 335— 343.
Review
For citations:
Vitruk T.Yu., Ryazantseva N.V., Pesterev P.N., Mustafina L.R. Features of the apoptosis markers expression by skin cell with ageing. Bulletin of Siberian Medicine. 2008;7(2):23-28. (In Russ.) https://doi.org/10.20538/1682-0363-2008-2-23-28
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