Receptor mechanism of infarct-limiting effect of adaptation to normobaric hypoxia
https://doi.org/10.20538/1682-0363-2020-4-138-142
Abstract
The aim of the study was to investigate the involvement of bradykinin, cannabinoid and vanilloid (TRPV1 channel) receptors in the implementation of the infarct-limiting effect of chronic normobaric hypoxia (CNH).
Materials and methods. The study was performed on male Wistar rats (n = 117) weighing 250–300 g. Adaptation to CNH was modeled for 21 days at 12% pO2, 0.3% pCO2 and normal atmospheric pressure. A day after adaptation of rats to CNH coronary artery occlusion (45 min) and reperfusion (2 h) was performed. In the study the following compounds were used: selective cannabinoid CB1 receptor antagonist rimonabant (1 mg/kg), selective cannabinoid CB2 receptor antagonist AM630 (2.5 mg/kg), selective bradykinin B2 receptor antagonist HOE140 (50 μg/kg), and vanilloid receptor (TRPV1 channel) antagonist capsazepine (3 mg/kg). All antagonists were administered 15 min before coronary artery occlusion.
Results. Adaptation to normobaric hypoxia promoted the formation of the pronounced infarct-limiting effect.
The blockade of B2 receptor eliminated the infarct-limiting effect of CNH. Blockade of cannabinoid or vanilloid
receptors did not affect the infarct-limiting effect of CNH.
Conclusion. The infarct-limiting effect of CNH depends on the activation of B2 receptor, and the adaptive increase in cardiac tolerance to ischemia/reperfusion does not depend on cannabinoid or vanilloid receptors.
About the Authors
N. V. Naryzhnaya
Cardiology Research Institute, Tomsk National Research Medical Center (TNRMC), Russian Academy of Science
Russian Federation
111a, Kievskaya Str., Tomsk, 634012, Russian Federation
Russian Federation
111a, Kievskaya Str., Tomsk, 634012, Russian Federation
A. V. Mukhomedzyanov
Cardiology Research Institute, Tomsk National Research Medical Center (TNRMC), Russian Academy of Science
Russian Federation
111a, Kievskaya Str., Tomsk, 634012, Russian Federation
Russian Federation
111a, Kievskaya Str., Tomsk, 634012, Russian Federation
S. Yu. Tsibulnikov
Cardiology Research Institute, Tomsk National Research Medical Center (TNRMC), Russian Academy of Science
Russian Federation
111a, Kievskaya Str., Tomsk, 634012, Russian Federation
Russian Federation
111a, Kievskaya Str., Tomsk, 634012, Russian Federation
L. N. Maslov
Cardiology Research Institute, Tomsk National Research Medical Center (TNRMC), Russian Academy of Science
Russian Federation
111a, Kievskaya Str., Tomsk, 634012, Russian Federation
Russian Federation
111a, Kievskaya Str., Tomsk, 634012, Russian Federation
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Review
For citations:
Naryzhnaya N.V., Mukhomedzyanov A.V., Tsibulnikov S.Yu., Maslov L.N. Receptor mechanism of infarct-limiting effect of adaptation to normobaric hypoxia. Bulletin of Siberian Medicine. 2020;19(4):138-142. https://doi.org/10.20538/1682-0363-2020-4-138-142
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