Gastroprotective effect of Ferulopsis hystrix (Bunge) Pimenov in ethanol-induced gastropathy

The aim of the study was to evaluate the gastroprotective effect of dry extracts from the roots and rhizomes of Ferulopsis hystrix in ethanol-induced gastropathy.

Materials and methods. The studies were carried out on 68 white Wistar rats. Ethanol-induced gastropathy was simulated by a single intragastric administration of ethanol in the dose of 10 ml/kg. Animals of the experimental groups received medicinal forms from the roots and rhizomes of F. hystrix: I – decoction in a volume of 10 ml/kg; II–V – dry extracts in the dose of 200 mg/kg, obtained by extraction with purified water, 30, 40 and 70% ethanol, respectively; VI – dry extract, prepared by double extraction with 40% and single extraction with 30% ethanol for 7 days before the modeling of gastropathy. Number of structural changes was determined in the gastric mucosa. They were differentiated into small, large, and strip-like erosions. The Pauls’ index was calculated for structural changes. Pathomorphological studies of the stomach were carried out.

Results. The total number of structural changes in the stomach of animals in experimental groups I, II, IV and V is 44% lower on average, in experimental group III it is 67% lower and in experimental group VI it is 3.6 times lower than in the control. The Pauls’ index for large erosions in experimental groups I–V is 38–75% lower, in experimental group VI it is 83% lower than the index in the control animals. No strip-like erosions are detected in animals of experimental groups III–VI. Pauls’ index for these destructions in experimental groups I and II is 7.0 and 6.5 times lower than the index in the control animals. Microscopic morphological examination registered the increase of shallow-like erosions in the stomach of animals of experimental groups. Shallow-like erosions do not reach the muscularis mucosae. Microcirculation disorders and leukocyte infiltration are less pronounced than in the control group.

Conclusion. F. hystrix has the gastroprotective effect, increasing the resistance of the gastric mucosa to the effect of ethanol. The F. hystrix extract prepared with 30% and 40% ethanol shows the most pronounced pharmacotherapeutic effect in ethanol-induced gastropathy.

1. Растительные ресурсы: Дикорастущие цветковые растения, их компонентный состав и биологическая активность. Т. 3. Семейство Fabaceae – Apiaceae; отв. ред. А.Л. Буданцев. СПб.; М., 2010: 601.

2. Серенот С.К. Тувинская народная медицина: лекарственные растения, травы, лишайники, грибы с параллельным описанием их использования в китайской, монгольской и тибетской медицинах. Кызыл: Тувин. кн. изд-во им. Ю.Ш. Кюнзегеша, 2009: 118.

3. Асеева Т.А., Кузнецова Н.А., Михневич Л.В., Корнопольцева Т.В., Чехирова Г.В. Болезни органов пищеварения: симптоматика и лечение (по материалам тибетских медицинских сочинений XII–XVII вв.). Новосибирск: Наука, 2016: 188.

4. Ganbaatar J., Shults E.E., Otgonsuren D., Radnaeva L.D. Taraskin V.V., Badamkhand D. Coumarins from Peucedanum hystrix growing in Mongolia. Mongolian Journal of Chemistry. 2014; 12: 42–49. DOI: 10.5564/mjc.v12i0.170.

5. Shul’ts E.E., Petrova T.N., Shakirov M.M., Bagryanskaya I.Yu., Taraskin V.V., Tolstikov G.A., Ganbaatar Zh., Radnaeva L.D., Otgonsuren D., Pokrovskii A.G. Plant coumarins IX. Phenolic compounds of Ferulopsis hystrix growing in Mongolia. Cytotoxic activity of 8,9- dihydrofurocoumarins. Chemistry of Natural Compounds. 2012; 48 (2): 211–217. DOI: 10.1007/s10600-012-0207-3.

6. Heide L. The aminocoumarins: biosynthesis and biology. Natural Product. Reports. 2009. 26 (10): 1241–1250. DOI:

7. 1039/b808333a.

8. Song P.-P., Zhao J., Liu Z.-L., Duan Ya.-B., Hou Yi.-P., Zhao Ch.-Q., Wu M., Wei M., Wang N.-H., Lv Ye., Han Z.-J. Evaluation of antifungal activities and structure-activity relationships of coumarin derivatives. Pest Management Science. 2017; 73 (1): 94–101. DOI: 10.1002/ps.4422.

9. Курбанова Ф.К., Серкеров С.В. Этерифицированные дигидрофурокумарины корней Seseli transcaucasica (Apiaceae). Растительные ресурсы. 2012; 48 (2): 228–233.

10. Brumbarova T., Bauer P., Ivanov R. Molecular mechanisms governing Arabidopsis iron uptake. Trends in Plant Science. 2015; 20 (2): 124–133. DOI: 10.1016/j.tplants.2014.11.004.

11. Tomasz K., Rafał P., Monika S. Natural and synthetic coumarins and their pharmacological activity. European Journal of Clinical and Experimental Medicine. 2017; 15 (2): 169–175. DOI: 10.15584/ejcem.2017.2.12.

12. Парфёнов Э.А., Трапков В.А., Шабанов П.Д. Редокс- регуляция как надежная платформа поиска и разработки лекарств нового типа. Поиск гастропротекторов среди замещенных кумаринов. Обзоры по клинической фармакологии и лекарственной терапии. 2014; 12 (4): 22–42.

13. Аракчаа К.Д., Николаев С.М., Салчак С.М., Разуваева Я.Г., Николаева И.Г., Торопова А.А., Николаева Г.Г. Способ получения средства, обладающего гастропротективной, противовоспалительной, антиоксидантной активностью. Патент на изобретение № 2679310. Заявка № 2018110207 от 22.03.2018. МПК A61K36/23 (2006.01). Опубликовано: 07.02.2019 Бюл. № 4.

14. Багинская А.И., Ферубко Е.В., Курманова Е.Н., Воскобейникова Е.Н., Сидельников Н.И. Экспериментальные модели эрозивно-язвенных поражений желудка и двенадцатиперстной кишки. М.: Издательский дом «Русский врач», 2017: 96.

15. Ibrar A., Shehzadi S.A., Khan I. Developing hybrid molecule therapeutics for diverse enzyme inhibitory action: аctive role of coumarin-based structural leads in drug discovery. Bioorganic & Medicinal Chemistry. 2018; 26 (13): 3731–3762. DOI: 10.1016/j.bmc.2018.05.042.

16. Chauhan S., Devi U., Kumar V.R., Anwar F., Kaithwas G. Dual inhibition of arachidonic acid pathway by mulberry leaf extract. Journal Inflammopharmacology. 2015; 23 (1): 65–70. DOI: 10.1007/s10787-014-0223-y.

17. Cheriyan B.V, Kadhirvelu P., Nadipelly J., Shanmugasundaram J., Sayeli V., Subramanian V. Anti- nociceptive effect of 7-methoxy coumarin from Eupatorium triplinervevahl (Asteraceae). Pharmacogn Magazine. 2017; 13 (49): 81–84. DOI: 10.4103/0973-1296.197650.

18. Barot Kuldipsinh P., Jain Shailesh V., Kremer L., Singh S. Recent advances and therapeutic journey of coumarins: current status and perspectives. Medicinal Chemistry Researsh. 2015; 24 (7): 2771–2798. DOI: 10.1007/s00044-015-1350-8.