Features of the cytokine profile in children with autism spectrum disorder
https://doi.org/10.20538/1682-0363-2020-4-174-178
Abstract
The aim of the study: to reveal the particularities of the concentration of cytokines IL4, IL6, IL10, IL17, IFNγ in blood serum in children with autism spectrum disorder (ASD).
Materials and methods. The blood samples obtained from children of two study groups: children with autism spectrum disorder (n = 93) and clinically healthy children (n = 30), served as the material for the study. Cytokine concentrations were determined in blood serum using the Bender Medsystems (Austria) kits for IL17A and Vector-Best (Russia) kits for IL4, IL6, IL10, IFNγ. Serum cytokine concentrations were determined by enzyme immunoassay using kits for IL17A (Bender Medsystems, Austria), IL4, IL6, IL10, IFNγ (Vector-Best, Russia). Assessment of cognitive and psychophysiological indicators in children was performed using the Autism Treatment Evaluation Checklist (ATEC).
Results. The concentrations of IL17A (U = 54; p = 0,015) and IFNγ (U = 4.64; p = 0,006) were increased and the concentrations of IL6 (U = 327; p = 0.001) and IL4 (U = 177; p = 0.001) were decreased in children with ASD. The concentration of IL6 correlates with the concentration of IL4 (r = 0.68; p < 0.05). The concentration of IL17A correlates with the concentration of IFNγ (r = 0.41; p < 0.05), IL6 (r = 0.87; p < 0.05) and ATEC score (r = 0.24; p < 0.05) in the group of children with ASD.
Conclusion. The cytokine disbalance in children with ADS, which was observed in our study, confirms the hypothesis of their participation in the development of the disease and clearly shows the Th17 immunoregulation pathway in the pathogenesis of the autism spectrum disorder.
Supporting agencies: The study was sponsored by the Center of Family Medicine (Tomsk, Russia), “Sozidaniye” charity fund (Moscow, Russia), and Association of Parents of Autistic Children “AURA” (Tomsk, Russia).
About the Authors
M. I. Khudiakova
Siberian State Medical University
Russian Federation
2, Moscow Trakt, Tomsk, 634055, Russian Federation
Russian Federation
2, Moscow Trakt, Tomsk, 634055, Russian Federation
N. A. Cherevko
Siberian State Medical University
Russian Federation
2, Moscow Trakt, Tomsk, 634055, Russian Federation
Russian Federation
2, Moscow Trakt, Tomsk, 634055, Russian Federation
P. S. Novikov
Siberian State Medical University
Russian Federation
2, Moscow Trakt, Tomsk, 634055, Russian Federation
Russian Federation
2, Moscow Trakt, Tomsk, 634055, Russian Federation
K. V. Berezovskaya
Center of Family Medicine
Russian Federation
22, Trifonova Str., Tomsk, 634055, Russian Federation
Russian Federation
22, Trifonova Str., Tomsk, 634055, Russian Federation
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Review
For citations:
Khudiakova M.I., Cherevko N.A., Novikov P.S., Berezovskaya K.V. Features of the cytokine profile in children with autism spectrum disorder. Bulletin of Siberian Medicine. 2020;19(4):174-178. https://doi.org/10.20538/1682-0363-2020-4-174-178
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