THE STRUCTURE OF THE LIPID PHASE OF THE ERYTHROCYTE MEMBRANE IN PATIENTS WITH EXPRESSED HEMOLYSIS AFTER SURGERY WITH CARDIOPULMONARY BYPASS
https://doi.org/10.20538/1682-0363-2013-1-75-79
Abstract
The composition of lipid phase of red-cell membrane in patients with ischemic heart disease (IHD) with pronounced postperfusion hemolysis (18 patients) before coronary artery bypass surgery and 1 hour after the completion of cardia bypass (CB) has been studied. It is shown that patients with IHD with pronounced hemolysis are characterized by the normal ratio of phospholipids (PL) fractions in red-cell membrane before surgery, which is connected with eth high content of young forms of red cells in blood at this stage. After surgery, the fraction of lysophosphatidylcholine and phosphatidic acid in red-cell membrane increases against the background of decrease of phosphatidylinositol and phosphatidylcholine, which likely reflects the simultaneous activation of phospholipases of three classes (A, C, and D) in red cells during CB. Regardless of the phase of the study, the total content of PL in red-cell membrane of IHD patients with pronounced hemolysis is decrease at the high level of cholesterol (CS) and the CS/Pl ratio.
About the Author
O. A. Khokhlov
Siberian State Medical University, Tomsk
Russian Federation
Khokhlov Oleg A., postgraduate student at the Chair of Pathophisiology
Russian Federation
Khokhlov Oleg A., postgraduate student at the Chair of Pathophisiology
References
1. Averina T.B., Samuilova D.Sh. Creative cardiology, 2007. no. 1–2, pp. 102–117 (in Russian).
2. Dutkevich I.G. The bulletin of surgery, 2007, vol. 166, no. 6, pp. 77–80 (in Russian).
3. Kamyshnikov V.S. Handbook of clinical and biochemical la-boratory diagnostics. Minsk, Belarus Publ., 2000, vol. 2, 495 p. (in Russian).
4. Kravets Ye.B., Stepovaya Ye.A., Koshhevets T.Yu. et al. Diabetes mellitus, 2010, no. 1, pp. 41–44 (in Russian).
5. Men'shikov V.V. Laboratory methods of investigation in clinic. Handbook. Moscow, Medicine Publ., 1987, 368 p. (in Russian).
6. Novitsky V.V., Ryazantseva N.V., Stepovaya Ya.A. Physio-logy and pathology of the red blood cell. Tomsk, TSY Publ., 2004, 202 p. (in Russian).
7. Rozhdestvenskaja M.A. Actual issuesof blood transfusion, 1955, no. 4, pp. 55. (in Russian).
8. Guide to hemotology. In 3 vol. Yu.N. Andreev, Z.S. Barkagan, A.Yu. Bulanov et al., ed. A.I. Vorob'ev. Moscow, Newdiamed Publ., 2005, vol. 3, 416 p. (in Russian).
9. Chumakova S.P., Urazova O.I., Novitsky V.V. et al. The Bulletin of RAMS, 2012, no. 7, pp. 15–19 (in Russian).
10. Chumakova S.P., Urazova O.I., Shipulin V.M. et al. The Bul-letin of Ural Medical Academic Science, 2011, vol. 33, no. 1, pp. 73–76 (in Russian).
11. Balsinde J., Balboa M.A., Insel P.A. et al. Regulation and ingibition of phospholipase A2. Annu. Rew. Pharmacol. Toxicol., 1999, vol. 39, pp. 175–189.
12. Dodge J.T., Mitchell C., Hanahan D.J. et al. The preparation and chemical characteristics of hemoglobin-free ghost of human erythrocytes. Archives Biochem. Biophys., 1963, vol. 100, no. 1, pp. 119–130.
13. Folch J., Lees M., Sloane-Stanley A.G.H. A simple method for the isolation and purification of total lipid from animal tissues. J. Biol. Chem., 1957, vol. 226, suppl. 1, pp. 497–509.
14. Liscotovitch M., Czarny M., Fiucci G. et al. Phospholipase D: molecular and cell biology of a novel gene family. Biochem. J., 2000, vol. 345, pp. 401–415.
15. Vercaemst L. Hemolysis in cardiac surgery patients undergo-ing cardiopulmonary bypass: A review in search of a treat-ment algorithm. The J. of Extra Corporeal Technology, 2008, vol. 40, no. 4, pp. 257–267.
Review
For citations:
Khokhlov O.A. THE STRUCTURE OF THE LIPID PHASE OF THE ERYTHROCYTE MEMBRANE IN PATIENTS WITH EXPRESSED HEMOLYSIS AFTER SURGERY WITH CARDIOPULMONARY BYPASS. Bulletin of Siberian Medicine. 2013;12(1):75-79. (In Russ.) https://doi.org/10.20538/1682-0363-2013-1-75-79
Views:
796
Email this article 