Proliferation and apoptosis of lymphocytes in response to borrelia antigen stimulation in Lyme-borreliosis patients

The object of this work was to study the correlation between the lymphocyte proliferation and apoptosis in the cultures stimulated by specific inactivated Borrelia garinii antigen and the different clinical outcomes in Lyme borreliosis patients. It was found that borrelia antigen stimulation of the cultures obtained from chronic patients induced T helper type 1-like response as well as depression in lymphocyte apoptosis, which may provide the basis of persistent activated T cell immune response and chronic inflammatory in Lyme borreliosis.

1. Гланц С. Медико-биологическая статистика. М.: Практика, 1998. 459 с.

2. Новиков Д.К., Новикова В.И. Оценка иммунного статуса. М.: Витебск — Москва, 1996. 286 с.

3. Рябченко А.В. Получение рекомбинантных белков западносибирских изолятов Borrelia burgdorferi sensu lato и изучение их антигенных свойств: автореф. дис. … канд. мед. наук. Новосибирск, 2009. 21 с.

4. Asbrink E., Hovmark A. Comments on the course and classi-fication of Lyme borreliosis // Scand. J. Infect. Dis. Suppl. 1991. V. 77. P. 41—43.

5. Sjöwall J., Carlsson A., Vaarala O. et al. Innate immune re-sponses in Lyme borreliosis: enhanced tumour necrosis factor-alpha and interleukin-12 in asymptomatic individuals in response to live spirochetes // Clin. Exp. Immunol. 2005. V. 141, № 1. P. 89—98.

6. Budd R.C. Activation-induced cell death // Curr. Opin. Im-munol. 2001. V. 13, № 3. P. 356—362.

7. Grygorczuk S., Osada J., Swierzbińska R. et al. Expression of Fas receptor on human T lymphocytes under stimulation with Borrelia burgdorferi sensu lato — preliminary results // Adv. Med. Sci. 2010. V. 55, № 2. P. 228—234.

8. Krause A., Brade V., Schoerner C. et al. T cell proliferation induced by Borrelia burgdorferi in patients with Lyme bor-reliosis. Autologous serum required for optimum stimulation // Arthritis Rheum. 1991. V. 34, № 4. P. 393—402.

9. Lacombe F., Beloc F., Bernard P., Boisseau M.R. Evalua-tion of four methods of DNA distribution data analysis based on bromdeoxyuridine / DNA bivariante data // Cytometry. 1988. V. 9, № 3. P. 245—253.

10. Oksi J., Savolainen J., Pene J. et al. Decreased interleukin-4 and increased gamma interferon production by peripheral blood mononuclear cells of patients with Lyme borreliosis // Infect. Immun. 1996. V. 64, № 9. P. 3620—3623.

11. Pohl-Koppe A., Balashov K., Steere A.C. et al. Identifica-tion of a T cell subset capable of both IFN-g and IL-10 se-cretion in patients with chronic Borrelia burgdorferi infection // J. Immunol. 1998. V. 160, № 4. P. 1804—1810.

12. Postic D., Assous M.V., Grimont P.A. et al. Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons // Int. J. Syst. Bacteriol. 1994. V. 44, № 4. P. 743—752.

13. Widhe M., Jarefors S., Ekerfelt C. et al. Borrelia-specific in-terferon-g and interleukin-4 secretion in cerebrospinal fluid and blood during Lyme borreliosis in humans: association with clinical outcome // J. Infect. Dis. 2004. V. 189, № 10. P. 1881—1891.