Transformation of NETs under the effect of pathogens and IgG
https://doi.org/10.20538/1682-0363-2024-4-22-30
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Abstract
Background. Many studies have shown that neutrophil extracellular traps (NETs) in the form of web-like structures are present in the peripheral blood of patients with inflammatory diseases. In our research, in addition to traditional web-like NET structures, several anomalous forms were identified, including NETs with cloud-like appearance.
Aim. To investigate morphological and functional transformation of NETs under the influence of Klebsiella pneumoniae and immunoglobulin G (IgG).
Materials and methods. The study included 42 patients of Moscow City Clinical Hospital No. 51: 28 patients with acute inflammation in the abdominal cavity (appendicitis, cholecystitis, pancreatitis, peritonitis), 6 patients diagnosed with ulcerative colitis, and 8 patients with hernias. Neutrophils were isolated using gradient-density centrifugation. To calculate NETs, we used SYBR Green I-induced fluorescence microscopy (Evrogen, Russia), with the dye specifically interacting with double-stranded DNA. The functional activity of NETs was determined in the Klebsiella pneumoniae (ATCC 700603) capture test.
Results. In patients with inflammatory diseases of the abdominal cavity in the postoperative period, the functional activity of NETs was several times lower than in healthy individuals. NETs in these patients capture and bind no more than 20 cells of the microorganism. Under the effect of IgG, neutrophil networks transform into loose cloud-like structures, which can hardly capture and bind the pathogen, binding only 8.46 ± 0.44 cells of the microorganism. Spontaneous enzymatic degradation of cloud like NETs may be accompanied by the production of secondary alteration factors.
Conclusion. The results of the study provide the grounds for the development of new approaches to elaborating vaccination regimens and using immunobiologics that require preliminary monitoring of the state of innate immunity, in particular, neutrophil networks in the patient’s body.
About the Authors
A. N. KazimirskiiRussian Federation
1, Ostrovityanova Str., Moscow ,117997
J. M. Salmasi
Russian Federation
1, Ostrovityanova Str., Moscow ,117997
G. V. Poryadin
Russian Federation
1, Ostrovityanova Str., Moscow ,117997
M. I. Panina
Russian Federation
1, Ostrovityanova Str., Moscow ,117997
A. E. Kim
Russian Federation
1, Ostrovityanova Str., Moscow ,117997
L. S. Rogozhina
Russian Federation
1, Ostrovityanova Str., Moscow ,117997
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Review
For citations:
Kazimirskii A.N., Salmasi J.M., Poryadin G.V., Panina M.I., Kim A.E., Rogozhina L.S. Transformation of NETs under the effect of pathogens and IgG. Bulletin of Siberian Medicine. 2024;23(4):22-30. https://doi.org/10.20538/1682-0363-2024-4-22-30
ISSN 1819-3684 (Online)