Spontaneous neutrophil extracellular trap formation during pollinosis exacerbation
A. N. Kazimirsky,
J. M. Salmasi,
G. V. Poryadin,
M. I. Panina,
A. E. Kim,
T. E. Kim,
O. O. Turishcheva https://doi.org/10.20538/1682-0363-2026-2-32-37
Abstract
Aim. To study the morphological structure and functional activity of neutrophil extracellular traps in patients with seasonal allergic rhinitis during the exacerbation period.
Materials and methods. The study included 12 patients with seasonal allergic rhinitis during the exacerbation period. We studied the morphological structure and functional activity of neutrophil extracellular traps (NET) in pollinosis patients. Fluorescence microscopy with SYBR Green dye was used to visualize NETs.
Results. The study of neutrophils from allergic patients during the exacerbation period showed spontaneous NETs formation in the morphological form of neutrophil networks. The number of NETs during 1 hour of incubation is 10 [8.3–14]%, and their size is 28 [17.5–35] μm. Culturing neutrophils from allergic patients for 2 hours does not increase the number of NETs, but the size of NETs doubles, and 10% of NETs form as single threads and fibers. Neutrophil networks have a high functional activity.
Conclusion. The results specify that during the exacerbation of seasonal allergic rhinitis, there is an NET formation similar to that observed during infectious inflammation, which is combined with high functional activity of neutrophil networks.
About the Authors
A. N. Kazimirsky
Pirogov Russian National Research Medical University (Pirogov University)
Russian Federation
Russian Federation
1 Ostrovityanov St., 117997 Moscow, Russian Federation
J. M. Salmasi
Pirogov Russian National Research Medical University (Pirogov University)
Russian Federation
Russian Federation
1 Ostrovityanov St., 117997 Moscow, Russian Federation
G. V. Poryadin
Pirogov Russian National Research Medical University (Pirogov University)
Russian Federation
Russian Federation
1 Ostrovityanov St., 117997 Moscow, Russian Federation
M. I. Panina
Pirogov Russian National Research Medical University (Pirogov University)
Russian Federation
Russian Federation
1 Ostrovityanov St., 117997 Moscow, Russian Federation
A. E. Kim
Pirogov Russian National Research Medical University (Pirogov University)
Russian Federation
Russian Federation
1 Ostrovityanov St., 117997 Moscow, Russian Federation
T. E. Kim
Pirogov Russian National Research Medical University (Pirogov University)
Russian Federation
Russian Federation
1 Ostrovityanov St., 117997 Moscow, Russian Federation
O. O. Turishcheva
Pirogov Russian National Research Medical University (Pirogov University)
Russian Federation
Russian Federation
1 Ostrovityanov St., 117997 Moscow, Russian Federation
References
1. Wang J., Zhou Y., Zhang H., Hu L., Liu J., Wang L. et al. Pathogenesis of allergic diseases and implications for therapeutic interventions. Sig. Transduct. Target Ther. 2023;8(1):138. DOI: 10.1038/s41392-023-01344-4.
2. Lianou P., Fortis A., Papadopoulos N., Trakas N. Defective neutrophil function in patients with perennial allergic rhinitis. Allergology International. 2001;50(1):15–20. DOI: 10.1046/j.1440-1592.2001.00196.x.
3. Arebro J., Ekstedt S., Hjalmarsson E., Winqvist O., Kumlien Georén S. et al. A possible role for neutrophils in allergic rhinitis revealed after cellular subclassification. Sci. Rep. 2017;7:43568. DOI: 10.1038/srep43568.
4. Казимирский А.Н., Салмаси Ж.М., Порядин Г.В., Панина М.И., Ким А.Э., Чахалян А.Г., Турищева О.О. ДНК-содержащие экстраклеточные структуры опухолевых клеток подавляют формирование нейтрофильных экстраклеточных ловушек in vitro. Бюллетень сибирской медицины. 2025;24(2):36–43.
5. Казимирский А.Н., Салмаси Ж.М., Порядин Г.В., Панина М.И., Ступин В.А., Ким А.Э. и др. Противоинфекционная защита организма человека с участием нейтрофильных сетей. Бюллетень сибирской медицины. 2024;23(1):56–63. DOI: 10.20538/1682-0363-2024-1-56-63
6. Keshari R.S., Jyoti A., Dubey M., Kothari N., Kohli M., Bogra J. et al. Cytokines induced neutrophil extracellular traps formation: implication for the inflammatory disease condition. PloS One. 2012;7(10):e48111. DOI: 10.1371/journal.pone.0048111.
7. Abrams S.T., Morton B., Alhamdi Y., Alsabani M., Lane S., Welters I.D. et al. A novel assay for neutrophil extracellular trap formation independently predicts disseminated intravascular coagulation and mortality in critically ill patients. American Journal of Respiratory and Critical Care Medicine. 2019;200(7):869–880. DOI: 10.1164/rccm.201811-2111OC.
8. Hosoki K., Brasier A.R., Kurosky A., Boldogh I., Sur S. Reply: protease plays a role in ragweed pollen-induced neutrophil recruitment and epithelial barrier disruption. Am. J. Respir. Cell Mol. Biol. 2017;56(2):272–273. DOI: 10.1165/rcmb.2016-0281LE.
9. Казимирский А.Н., Салмаси Ж.М., Порядин Г.В., Панина М.И., Ким А.Э., Рогожина Л.С. Нейтрофильные экстраклеточные ловушки как маркеры инфекционного и асептического воспаления. Инфекция и иммунитет. 2024;14(3):505–510. DOI: 10.15789/2220-7619-NET-16602.
10. Казимирский А.Н., Салмаси Ж.М., Порядин Г.В., Панина М.И., Ким А.Э., Рогожина Л.С. Трансформация нейтрофильных сетей под влиянием патогенов и иммуноглобулинов класса G. Бюллетень сибирской медицины. 2024;23(4):22–30. DOI: 10.20538/1682-0363-2024-4-22-30.
Review
For citations:
Kazimirsky A.N., Salmasi J.M., Poryadin G.V., Panina M.I., Kim A.E., Kim T.E., Turishcheva O.O. Spontaneous neutrophil extracellular trap formation during pollinosis exacerbation. Bulletin of Siberian Medicine. 2026;25(2):32-37. https://doi.org/10.20538/1682-0363-2026-2-32-37
Views:
47
JATS XML
Email this article 