Hystochrome influence on physical endurance of rats
https://doi.org/10.20538/1682-0363-2016-4-97-103
Abstract
Introduction. Domestic antioxidant drugs series «Gistohrom®» are based on the marine natural compounds echinochrome A. «Gistohrom®»activates cell energy metabolism and protein synthesis, increases the body’s endurance under extreme physical exertion. The aim of the research was to study effect of different «Gistohrom®» doses on the physical endurance rats.
Material and Methods The male Wistar rats were ranked into three groups: group 1 (n = 17) and group 2 (n = 15) for 10 days subcutaneously treated «Gistohrom®» on doses 1 mg/kg and 10 mg/kg, respectively, while the control group (n = 20) received the same quantity of saline injections. Physical endurance (efficiency) was evaluated by the test swim.
Results Repeated exposure to extreme physical exertion accompanied by increased efficiency in all groups of animals. In the control group of rats swimming duration increased to 9,31 ± 0,39 min (p < 0,001) and reached maximum values at the 9th day of the experiment. Physical endurance in rats exceeded by 1,7 times comparison reference values when «Gistohrom®» administered at a dose of 10 mg/kg, and by 2,7 times at a 1 mg/kg drug dose. Maximum growth endurance was recorded at 25,59 ± 3,87 minutes after the cancellation of «Gistohrom®» applied in a dose of 10 mg/kg, and at 28,24 ± 4,23 minutes after its administration at a dose of 1 mg/kg.
Conclusion. The increase of the time water retention in the control rats group, appears to be the result of long-term adaptation to physical exercise. Increase physical endurance in rats with long-term administration «Gistohrom®» inversely proportional to the administered dose. Perhaps the key mechanism is to modulate the p53 gene activity by echinochrome A.
About the Authors
O. S. Talalaeva
Altay State Medical University 40, Leninа St., Barnaul, 656038, Russian Federation
Russian Federation
PhD, Department of Pathophysiology, Altay State Medical University, Russian Federation
Russian Federation
PhD, Department of Pathophysiology, Altay State Medical University, Russian Federation
Ya. F. Zverev
Altay State Medical University 40, Leninа St., Barnaul, 656038, Russian Federation
Russian Federation
MD, Professor, Department of Pharmacology, Altay State Medical University, Russian Federation
Russian Federation
MD, Professor, Department of Pharmacology, Altay State Medical University, Russian Federation
V. M. Bryukhanov
Altay State Medical University 40, Leninа St., Barnaul, 656038, Russian Federation
Russian Federation
MD, Professor, Department of Pharmacology, Altay State Medical University, Russian Federation
Russian Federation
MD, Professor, Department of Pharmacology, Altay State Medical University, Russian Federation
References
1. Mishenko N.P., Fedoreev S.A., Bagirova S.l. Novij originalnij otechestvennij preparat gistochrom [New original domestic drug histochrome] // Chim.-pharmacevt. Journ, 2003; 37 (1): 49–53 (in Russian).
2. http://grls.rosminzdrav.ru/Grls_View_v2.aspx-?idReg=38019&t=
3. Enciklopedia lekarstvennich sredstv / Yu.L. Shevchenko et al. M.: Remedium Rubl., 2001: 1775 (in Russian).
4. Egorov G.B., Alehina V.A., Volobueva T.M. Novij bioantioxidant gistochrom v klinike glasnih bolesnei [New bioantioxidant histochrome in clinic of eye diseases] // Vestnik ophthalmologii, 1999; 115 (2): 34–35 (in Russian).
5. Zakirova A.N., Ivanova M.V., Golubiatnikova V.B. Pharmakokinetika i klinicheskaia effektivnist gistochroma u bolnich ostrim infarktom miokarda [Pharmacokinetics and clinical efficacy histochrome in patients with acute myocardial infarction] // Experimentalnaia I klinicheskaia pharmakologia. 1997; 60 (4): 21–24 (in Russian).
6. Polunin G.S., Vorobjeva O.K., Makasheva N.V. Opit primenenia preparata gistochrom v ophthalmologicheskoi praktike [Experience with histochrome drug in ophthalmic practice] // Refrakcionnaia chirurgia I ophthalmologia. 2003; 3 (2): 23–28 (in Russian).
7. Repnin A.N., Maximov I.V., Markov V.A. i dr. Ocenka kardioprotektornogo deistvia emoxipina pri tromboliticheskoi reperfusii moikarda [Evaluation of cardioprotective action emoxipine myocardial reperfusion with thrombolytic] // Kardiologia – Cardiology. 1994; 3: 4–7 (in Russian).
8. Koslov V.K., Koslov M.V., Lebedko O.A. i dr. Kliniko-experimentalnoe obosnovanie primenenia echinochroma A dlia korrekcii i profilaktiki strukturno-methabolicheskich narushenij v sisteme organov dichania na rannih etapah ontogensa [Clinical and experimental substantiation of application echinochrome A for correction and prevention of structural and metabolic disorders in the respiratory system in the early stages of ontogeny] // Dalnevostochnii medicinskii Journal. 2009; 2: 61–64 (in Russian).
9. Talalaeva O.S., Garikov A.U., Fedoreev S.A. i dr. Vlianie gistochroma na exkretornuju funkciju pochek v experimente [Influence histochrome on renal excretory function in the experiment] // Bull. Sibirskoi medicini, 2011; 5: 101–104 (in Russian).
10. Mishenko N.P., Fedoreev S.A., Stonik V.A. i dr. Preparat dlja lechenia gemorragicheskogo insulta i sposob lechenia gemorragicheskogo insulta [Drug for the treatment of hemorrhagic stroke and method for the treatment of hemorrhagic stroke] // Pat. № 2266737 RF.; opubl. 12.04.2004 (in Russian).
11. Zakirova A.N., Lebedev A.V., Kuharchuk V.V. i dr. Antioxidant gistochrom: vlianie na perekisnoe okislenie lipidov i reologicheskie svoistva krovi u bolnih nestabilnoi stenokardiei [Antioxidant histochrome: effects on lipid peroxidation and blood rheology in patients with unstable angina] // Terapevticheskii archive. 1996; 68 (8): 12–14 (in Russian).
12. Lebedev A.V., Leviclaia E.L., Tichonova E.V. i dr. Antioxidantnie svoistva, avtookislenie i mutagennaia aktivnost echinohroma A v sravnenii s ego esterificirovannim analogom [The antioxidant properties and autoxidation echinochrome A mutagenic activity compared with its analogue esterified] // Biohimia – Biochemistry. 2001; 66 (8): 1089–1098 (in Russian).
13. Lebedev A.V., Ivanova M.V., Levitsky D.O. Echinochrome, a naturally occurring iron chelator and free radical scavenger in artificial and natural membrane systems // Life Sci. 2005; 76 (8): 863–875.
14. Kareeva E.N., Tihonov D.A., Mishenko N.P., Fedoreev S.A., Shimanovslij N.L. Vlianie gistochroma na expressiu p53 v kletkah krasnogo kostnogo mosga michei v usloviah modeli hronicheskogo stressa [Effects of Histochrome on the expression of p53 in the cells of the red bone marrow of mice in a model of chronic stress] // Chim.-pharmacevt. Journ. 2014; 48 (3): 9–12.
15. Lennikov A., Kitaichi N., Noda K., et al. Amelioration of endotoxin-induced uveitis treated with the sea urchin pigment echinochrome in rats // Mol Vis. 2014; 2 (20): 171–177.
16. Eremenko E.M., Antimonova O.I., Shekalova O.G., et al. Novel compounds increasing chaperone Hsp70 expression and their biological activity // Tsitologiia. 2010; 52 (3): 235–241.
17. Miroshnikov S.V., Notova S.V., Timasheva A.B., Manshins L.E. Pokasateli adaptivnosti k phisicheskoi nagruske laboratornih jivotnih v usloviah experimentalnogo ismenenia tireoidnogo statusa [Indicators of adaptation to physical stress in laboratory animals in experimental changes in thyroid status] // Medicinskie nauki. Fundamentalnie issledovania, 2012; 10: 73–77 (in Russian).
18. Seo D.Y., McGregor R.A., Noh S.J., et al. Echinochrome A Improves Exercise Capacity during Short-Term Endurance Training in Rats // Mar. Drugs. 2015; 13: 5722–5731. doi:10.3390/md13095722.
19. Kim H.K., Youm J.B., Jeong S.H., et al. Echinochrome A regulates phosphorylation of phospholamban Ser16 and Thr17 suppressing cardiac SERCA2A Ca²- reuptake // Pflugers Arch. 2015; 467 (10): 21514672163. doi: 10.1007/s00424-014-1648-2. Epub 2014 Nov 21.
20. Dawson C.A., Horvath S.M. Swimming in small laboratory animals // Med Sci Sports. 1970; 2: 51–78.
21. Horvath S.M., Dawson C.A., Lin Y.C. Regional distribution of 137Cs in rats forced to swim in warm and cold water // Comp Biochem Physiol. 1970; 37 (2): 265– 270.
22. Jeltuhin A.O., Chumakov P.M. Povsednevnie i induciruemie funkcii gena p53 [Casual and inducible p53 gene function] // Uspehi biologicheskoi himii. 2010; 50: 447– 526.
23. Artiukov A.A., Popov A.M., Tsybul’skiĭ A.V., et al. [Pharmacological activity echinochrome A singly and consisting of BAA “Timarin”] // Biomed Khim. 2012; 58 (3): 281–290.
24. Talalaeva O.S., Mishenko N.P., Bruchanov V.M. i dr. Vlijanie gistochroma na process svobodnoradikalnogo okislenia v experimente [Effect histochrome the process of free radical oxidation in experiment] // Bull. SO RAMN. 2011; 3 (31): 63–67 (in Russian).
25. Afanaseva T.A., Lasukova T.V., Cherniavskii A.M. ATF-sberegauchij effect gistochroma pri ostroi ishemii miokarda bolnih ishemicheskoi bolesniu serdca [ATP-saving effect histochrome in acute myocardial ischemia in coronary heart disease] // Bull. experimentalnoi biologii I medicini – Bulletin of Experimental Biology and Medicine. 1997; 124 (12): 669–676 (in Russian).
26. Talalaeva O.S., Zverev Y.F., Zamyatin S.V., Bruchanov V.M. i dr. Vlijanie gistochroma na osmoticheskuiu resistentnost eritrocitov v experimente in vitro in vivo [Effect histochrome on osmotic resistance of red blood cells in experiments in vitro in vivo] // Sib.med magazine. 2012; 27 (4): 70–74 (in Russian).
Review
For citations:
Talalaeva O.S., Zverev Ya.F., Bryukhanov V.M. Hystochrome influence on physical endurance of rats. Bulletin of Siberian Medicine. 2016;15(4):97-103. (In Russ.) https://doi.org/10.20538/1682-0363-2016-4-97-103
Views:
1020
Email this article 