Morphology of exorbital lacrimal glands of not purebred white rats at high-intensity light influence
https://doi.org/10.20538/1682-0363-2016-5-97-104
Abstract
Purpose: this study morphological changes the exorbital lacrimal glands at experimental impact on an organ of vision of high-intensity light.
Material and methods. White not purebred rats males (n = 40) were subjected to continuous influence of light intensity by 3500 lx within 1, 7, 14 and 30 days, the control group (n = 10) contained in conditions of natural lighting (20 lx). Exorbital lacrimal glands fixed in formalin, embedded in paraffin wax, stained hematoxylin-eosine. By means of an oculary insert of Avtandilov counted specific volumes of output channels, an epithelium, stroma and vessels of exorbital lacrimal glands, defined an epitelio/stroma ratio. The analysis of data was carried out by methods of descriptive statistics with calculation of a median (Ме) and interquartile range (Q1 –Q3). For an assessment of distinctions used nonparametric criterion of Mann – Whitney.
Results. In 1 days in response to high-intensity light influence there is a reflex strengthening of synthetic function of exorbital lacrimal glands. For the 7th days the first signs of violation of functioning with development of hydropic dystrophy in the lacrimacytes appear. By 14th days fibrous changes develop, in a glandular parenchyma harderization sites appear. For the 30th days the nuclear pycnosis of separate lacrimacit, accumulation of a lipofuscin in cytoplasm is observed.
Conclusion. The obtained data allow to estimate in dynamics compensatory and adaptive reactions of lacrimal gland in response to high-intensity light influence from the moment of the beginning of their development before emergence of the first signs of a decompensation.
About the Authors
L. R. Mustafina
Siberian State Medical University
Russian Federation
Russian Federation
DM, Professor of Department of Histology, Embryology and Cytology,
2, Moscow Trakt, Tomsk, 634050
A. D. Zhuravleva
Siberian State Medical University
Russian Federation
Russian Federation
5-year Student,
2, Moscow Trakt, Tomsk, 634050
S. V. Logvinov
Siberian State Medical University
Russian Federation
Russian Federation
DM, Рrofessor, Head of Department of Histology, Embryology and Cytology,
2, Moscow Trakt, Tomsk, 634050
M. R. Karpova
Siberian State Medical University
Russian Federation
Russian Federation
MD, Рrofessor, Head of Department Microbiology and Virology,
2, Moscow Trakt, Tomsk, 634050
T. V. Lasukova
Tomsk State Pedagogical University
Russian Federation
Russian Federation
DBSс, Professor, Department of Biomedical Sciences,
60а, Kievskaya Str., Tomsk, 634061
G. A. Sukhanova
Siberian State Medical University
Russian Federation
Russian Federation
DBSс, Professor, Biochemistry and Molecular Biology Department,
2, Moscow Trakt, Tomsk, 634050
N. N. Ilinskich
Siberian State Medical University
Russian Federation
Russian Federation
DBSс, Рrofessor, Head of Department Biology and Genetics,
2, Moscow Trakt, Tomsk, 634050
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Review
For citations:
Mustafina L.R., Zhuravleva A.D., Logvinov S.V., Karpova M.R., Lasukova T.V., Sukhanova G.A., Ilinskich N.N. Morphology of exorbital lacrimal glands of not purebred white rats at high-intensity light influence. Bulletin of Siberian Medicine. 2016;15(5):97-104. (In Russ.) https://doi.org/10.20538/1682-0363-2016-5-97-104
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