Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells
https://doi.org/10.20538/1682-0363-2016-5-105-112
Abstract
The purpose of this work is to study the influence of extracellular ATP (adenosine-3-phosphate), which is an activator of purinergic receptors, on contractive activity of rat aortic ring segments precontracted by α1-adrenoreceptors activation with phenylephrine and evaluate the impact of potassium channels of plasma membrane on mechanisms of ATP activity.
Material and methods. Contractive activity of vascular smooth muscle cells was studied using the method of Organ Bath Myography applied to the thoracic aorta segments in male Wistar rats both with intact and removed endothelium. ATP (1–1000 mM) produced a dose-dependent relaxing effect on intact and endothelium-denuded segments precontracted with phenilephrine. To assess the impact of potassium channels on mechanisms of ATP activity we used tetraethylammonium (10 mM), a nonselective potassium-channel blocker, glibenclamide (10 mM), the blocker of ATP-sensitive potassium channels and 4-aminopiridine, a blocker of voltage-gated potassium channels.
Conclusion. Our study has shown that the impact of ATP on segments with intact endothelium depends on the ATP-sensitive potassium channels whereas the impact of ATP on endothelium-denuded aortic segments depends on both ATP-sensitive and voltage-gated potassium channels.
About the Authors
S. N. Orlov
Siberian State Medical University;
Lomonosov Moscow State University
Russian Federation
Russian Federation
DBSс, Professor, Research Officer, 2, Moscow Trakt, Tomsk, 634050;
Professor, 1, Leninskiye Goru, Moscow, 119991
L. V. Smagliy
Siberian State Medical University
Russian Federation
Russian Federation
PhD, Associate Professor, Department of Biophysics and Functional Diagnostics,
2, Moscow Trakt, Tomsk, 634050
S. V. Gusakova
Siberian State Medical University
Russian Federation
Russian Federation
MD, the Chief of Department of Biophysics and Functional Diagnostics,
2, Moscow Trakt, Tomsk, 634050
V. S. Rydchenko
Siberian State Medical University
Russian Federation
Russian Federation
Postgraduate Student, Department of Biophysics and Functional Diagnostics,
2, Moscow Trakt, Tomsk, 634050
Yu. G. Birulina
Siberian State Medical University
Russian Federation
Russian Federation
Assistant Department of Biophysics and Functional Diagnostics,
2, Moscow Trakt, Tomsk, 634050
A. N. Baikov
Siberian State Medical University
Russian Federation
Russian Federation
MD, Нead of Central Research Laboratory,
2, Moscow Trakt, Tomsk, 634050
V. N. Vasiliyev
Siberian State Medical University
Russian Federation
Russian Federation
DBSс, Professor, Department of Physical Culture and Health,
2, Moscow Trakt, Tomsk, 634050
G. A. Sukhanova
Siberian State Medical University
Russian Federation
Russian Federation
DBSс, Professor, Biochemistry and Molecular Biology Department,
2, Moscow Trakt, Tomsk, 634050
T. S. Fedorova
Siberian State Medical University
Russian Federation
Russian Federation
MD, Professor, Biochemistry and Molecular Biology Department,
2, Moscow Trakt, Tomsk, 634050
T. V. Lasukova
Tomsk State Pedagogical University
Russian Federation
Russian Federation
DBSс, Professor, Department of Biomedical Sciences,
60a, Kievskaya Str., Tomsk, 634061
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Review
For citations:
Orlov S.N., Smagliy L.V., Gusakova S.V., Rydchenko V.S., Birulina Yu.G., Baikov A.N., Vasiliyev V.N., Sukhanova G.A., Fedorova T.S., Lasukova T.V. Role of potassium conductance in mechanisms of exrtacellular atp impact on the contractive activity of vascular smooth muscle cells. Bulletin of Siberian Medicine. 2016;15(5):105-112. (In Russ.) https://doi.org/10.20538/1682-0363-2016-5-105-112
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