Antigenic properties estimation for legumain protein recombinant analogue of trematoda Opisthorchis felineus, causing human opisthorchiasis
https://doi.org/10.20538/1682-0363-2012-6-166-171
Abstract
We estimated potential of protein legumain from opisthorchiasis agent O. felineus for application in immunodiagnosis of this disease. Bacterially expressed recombinant protein (rOF49-leg) showed strong difference in Enzyme-linked immunosorbent assay (ELISA) between infected and negative sera. These results suggest the potential of this antigen for development of serodiagnostic test for human opisthorchiasis.
About the Authors
I. A. Razumov
Институт цитологии и генетики СО РАН, г. Новосибирск
Russian Federation
Russian Federation
M. N. Lvova
Институт цитологии и генетики СО РАН, г. Новосибирск
Russian Federation
Russian Federation
Ye. P. Ponomareva
Институт цитологии и генетики СО РАН, г. Новосибирск
Russian Federation
Russian Federation
A. V. Katokhin
Институт цитологии и генетики СО РАН, г. Новосибирск
Russian Federation
Russian Federation
V. A. Petrenko
Институт цитологии и генетики СО РАН, г. Новосибирск
Russian Federation
Russian Federation
A. E. Sazonov
Институт цитологии и генетики СО РАН, г. Новосибирск
Russian Federation
Russian Federation
L. M. Ogorodova
Сибирский государственный медицинский университет, г. Томск
Russian Federation
Russian Federation
V. V. Novitsky
Сибирский государственный медицинский университет, г. Томск
Russian Federation
Russian Federation
A. Yu. Sivkov
Институт цитологии и генетики СО РАН, г. Новосибирск
Russian Federation
Russian Federation
V. A. Mordvinov
Институт цитологии и генетики СО РАН, г. Новосибирск
Russian Federation
Russian Federation
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For citations:
Razumov I.A., Lvova M.N., Ponomareva Ye.P., Katokhin A.V., Petrenko V.A., Sazonov A.E., Ogorodova L.M., Novitsky V.V., Sivkov A.Yu., Mordvinov V.A. Antigenic properties estimation for legumain protein recombinant analogue of trematoda Opisthorchis felineus, causing human opisthorchiasis. Bulletin of Siberian Medicine. 2012;11(6):166-171. (In Russ.) https://doi.org/10.20538/1682-0363-2012-6-166-171
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