Efficacy of n-tyrozol medication for regeneration stimulation of lip mucous membrane at experimental stomatitis

The purpose of the study is to investigate the effectiveness of the use of n-tyrosol (extract of rhodiola rosea) in the form of gel on the mucous membrane of the lip in rats with experimental stomatitis.
Materials and methods. The experiments were carried out on 54 white male rats weighing 220–280 g. The animals were divided into three groups: control, experimental for comparison and experimental main. When modeling chemical stomatitis in rats by applying 5% solution of caustic sodium to the mucous membrane of the lip, the development of inflammation was observed. The local application of the gel based on n-tyrosol was carried out three times a day for 12 days.
Histological and macroscopic examination was performed on the 3rd, 7th and 12th days. The extent of damage was recorded by measuring the area of the lesion in square millimeters (mm2 ). At the same time a control material was taken and was fixed in formalin, embedded into paraffin, stained with hematoxylin and eosin by Van Gieson. Specific areas of dense unformed connective tissue, lymphomonocytic infiltration, granulation tissue were determined in the lesion and surrounding tissues; the numerical density of the vessels was determined in 1 mm2 . The data obtained during the study of the dynamics of healing were averaged in the groups mentioned above and subjected to statistical processing using nonparametric (Mann–Whitney and Wilcoxon) methods using the computer program Statistica 8.0.
Results. On the 12th day, the area of the lesions was more than twice as large as the surface of burn surfaces, on which the gel with n-tyrosol was used. The use of n-tyrosine-based gel applications reduced by half the specific area of lymphomonocytic infiltration in the surrounding tissues and in the lesion itself, and promoted a more rapid maturation of the connective tissue. Quantitative analysis revealed that the use of the gel with n-tyrosol stimulated the proliferation of granulation and connective tissue, as well as the growth of newly formed vessels in the burn area. Thus, topical application of the n-tyrosine-based gel helped to reduce the inflammatory response and stimulated the regeneration process.

1. Shashy R.G., Ridley M.B. Aphthous ulcers: A difficult clinical entity // J. Am. Otolaryngol. 2000; 93. DOI: 10.1053/ajot.2000.18872.

2. Shulman J.D., Beach M.M., Rivera-Hidalgo F. The prevalence of oral mucosal lesions in U.S. adults: data from the Third National Health and Nutrition Examination Survey // J. Am. Dent. Assoc. 2004; 86. DOI: 10.14219/jada.archive.2004.0403.

3. Ming D.S. Hillhouse В.J., Guns E. S., Eberding A., Xie S., Vimalanathan S., Towers G.H. Bioactive compounds from Rhodiola rosea (Crassulaceae) // Phytother. Res. 2005;19 (9): 22. DOI:10.1002/ptr.1597.

4. Doncheva N.D., Mihaylova A.S., Getova D.P. Antinociceptive and anti-inflammatory effects of Rhodiola rosea L. Extract in rats // Folia Med. 2013; 55: 70–75. DOI: 10.2478/folmed-2013-0030.

5. Chernyshova G.A., Plotnikov M.B., Smoliakova V.I., Krasnov E.A. Main pharmacokinetic parameters of p-tyrosol after intravenous injection in rats. Part III: Distribution of p-tyrosol in rat // Eksp. Klin. Farmakol. 2011; 74: 27–29. DOI: 10.1007/s10517-007-0215-7.

6. Plotnikov M.B., Maslov M.Yu., Chernysheva G.A., Smol’yakova V.I., Aliev O.I.E, Vasil’ev A.S., Krysin A.P., Salakhutdinov N.F., Tolstikov G.A., Tolstikova T.G. Gemoreologicheskoe i antitrombotsitarnoe sredstvo [Hemorheological and antiplatelet agent]. Patent RUS 2239423. 2004 (in Russian).

7. Mendonça R.J.D., Coutinho-Netto J. Cellular aspects of healing // An. Bras. Dermatol. 2009; 84 (3): 257–262. DOI: 10.1590/s0365-05962009000300007.

8. Levitskiy A.P., Den’ga O.V., Makarenko O.A., Khromagina L.N., Stupak E.P., Tomilina T.V., Knava O.E. Eksperimental’nye metody vosproizvedeniya gingivita [Experimental methods gingivitis] // Innovatsii v stomatologii – Innovations in Dentistry. 2013; 1: 2–6 (in Russian).

9. Mazanova L.S., Abakumova T.A. Spasov A.A., Temkin E.S., Novochadov V.V. Izuchenie effektivnosti gelya «polikatan» pri travmaticheskom stomatite [The study of the effectiveness of the gel “polikatan” traumatic stomatitis] // Saratovskiy nauchno-meditsinskiy zhurnal – Saratov Journal of Medical Scientific Research. 2008; 4: 75–77 (in Russian).

10. Popkov V.M., Chesnokova N.P., Ledvanov M.Yu. Aktivatsiya lipoperoksidatsii kak vedushchiy patogeneticheskiy faktor razvitiya tipovykh patologicheskikh protsessov i zabolevaniy razlichnoy etiologii [Activation of lipid peroxidation as a leading pathogenetic factor in the development of typical pathological processes and diseases of different etiologies]. Saratov: SGMU, 2012 (in Russian).

11. Sarris J., Panossian A., Schweitzer I., Stough C., Schley A. Herbal medicine for depression, anxiety and insomnia: a review of psychopharmacology and clinical evidence // Eur. Neuropsychopharmacol. 2011; 21: 841–860. DOI: 10.1016/j.euroneuro.2011.04.002.

12. Konstantinova E.E. Osnovnye zakonomernosti izmeneniya reologicheskikh svoystv krovi i sostoyaniya mikrotsirkulyatsii u bol’nykh ishemicheskoy bolezn’yu serdtsa v usloviyakh dislipoproteinemiy [The basic laws of change blood rheology and microcirculation state in patients with coronary heart disease in a dislipoproteinemia] // Tromboz, gemostaz, reologiya – Thrombosis, hemostasis, reologiya. 2004; 18 (12): 62–68 (in Russian).

13. Golubeva I.V. Mekhanizmy kardioprotektornogo deystviya i parametry farmakokinetiki p-tirozola: аvtoref. dis. ... kand. biol. nauk [Cardioprotective mechanisms of action and pharmacokinetics parameters of p-tyrosol: аuthor. dis. ... cand. biol. scien]. Tomsk, 2007 (in Russian).

14. Radomska-Leśniewska D.M., Skopiński P., Bałan B.J., Białoszewska A., Jóźwiak J., Rokicki D., Skopińska-Różewska E., Borecka A., Hevelke A. Angiomodulatory properties of Rhodiola spp. and other natural antioxidants // Cent. Eur. J. Immunol. 2015; 40 (2): 249–262. DOI: 10.5114/ceji.2015.52839.