Protective effect of grapes polyphenol concentrate “Fenokor” in terms of hypoxic myocardial injury
https://doi.org/10.20538/1682-0363-2017-3-34-42
Abstract
Polyphenols of grapes and their beneficial effects on human health have been known for a long time and still attract more and more research interest.
The aim of the research was to reveal cardioprotective properties of polyphenols contained in grape concentrate “Fenokor” in terms of experimental histotoxic hypoxia.
Materials and methods. The study was conducted on 21 adult male Wistar rats divided into 3 groups, 2 of which were administered CoCl2 aqueous solution in a dose of 60 mg/kg for 7 days intragastrically. The control group consisted of 5 intact animals. The rats of the second group (n = 8) after the introduction of the cobalt did not receive any treatment, the animals of the third group (n = 8) after the administration of cobalt chloride intragastrically, were given Fenokor aqueous solution at a dose of 2,5 ml/kg along with 0,05 ml of water orally. Morphological study was performed using light and electron microscopy. During the experiment the following biochemical parameters such as contents of malondialdehyde, oxidative modification of proteins were estimated.
The results. The result of the influence of cobalt on the heart of animals in experiments is the development of severe cardiomyopathy that requires cardio-protection. Histological structure of myocardium observed in the second group of male rats after cobalt intoxication on the background of grape polyphenol concentrate generally reflected a tendency to minimize the damage extent which was manifested in the form of normalization of cell structures and muscle fibers.
Application of Fenokor has demonstrated its antioxidant and cytoprotective properties, which contributed to myocardial structure preservation in rats exposed to histotoxic hypoxia.
About the Authors
Igor V. Zadnipryany
Medical Academy named after S.I. Georgievskiy, Crimean Federal University (CFU) named after V.I. Vernadsky
Russian Federation
DM, Professor, Head of the Topographical Anatomy Department, Medical Academy named after S.I. Georgievskiy, CFU named after V.I. Vernadsky, Simferopol, Republic of Crimea, Russian Federation.
Russian Federation
DM, Professor, Head of the Topographical Anatomy Department, Medical Academy named after S.I. Georgievskiy, CFU named after V.I. Vernadsky, Simferopol, Republic of Crimea, Russian Federation.
Olga S. Tretiakova
Medical Academy named after S.I. Georgievskiy, Crimean Federal University (CFU) named after V.I. Vernadsky
Russian Federation
DM, Professor, Head of the Public Health and Healthcare Department, Medical Academy named after S.I. Georgievskiy, CFU named after V.I. Vernadsky, Simferopol, Republic of Crimea, Russian Federation.
Russian Federation
DM, Professor, Head of the Public Health and Healthcare Department, Medical Academy named after S.I. Georgievskiy, CFU named after V.I. Vernadsky, Simferopol, Republic of Crimea, Russian Federation.
Anatoliy V. Kubyshkin
Medical Academy named after S.I. Georgievskiy, Crimean Federal University (CFU) named after V.I. Vernadsky
Russian Federation
DM, Professor, Head of the General and Clinical Pathophysiology Department, Medical Academy named after S.I. Georgievskiy, CFU named after V.I. Vernadsky, Simferopol, Republic of Crimea, Russian Federation.
Russian Federation
DM, Professor, Head of the General and Clinical Pathophysiology Department, Medical Academy named after S.I. Georgievskiy, CFU named after V.I. Vernadsky, Simferopol, Republic of Crimea, Russian Federation.
Tatiana P. Sataieva
Medical Academy named after S.I. Georgievskiy, Crimean Federal University (CFU) named after V.I. Vernadsky
Russian Federation
DM, Associate Professor, Medical Biology Department, Medical Academy named after S.I. Georgievskiy, CFU named after V.I. Vernadsky, Simferopol, Republic of Crimea, Russian Federation
Russian Federation
DM, Associate Professor, Medical Biology Department, Medical Academy named after S.I. Georgievskiy, CFU named after V.I. Vernadsky, Simferopol, Republic of Crimea, Russian Federation
References
1. Nichols M., Townsend N., Scarborough P., Rayner M. Cardiovascular disease in Europe 2014: epidemiological update // European Heart Journal. 2014; 1:1–10.
2. Buja L.M. The pathobiology of acute coronary syndromes: clinical implications and central role of the mitochondria // Tex. Heart Inst. J. 2013; 40 (3): 221–228.
3. Hatori N., Pehrsson S.K., N. Clyne N. et al. Acute cobalt exposure and oxygen radical scavengers in the rat myocardium // Biochimica et Biophysica Acta. 1993; 1181: 257–260.
4. Bruick R.K. Oxygen sensing in the hypoxic response pathway: regulation of the hypoxia-inducible transcription factor // Genes Dev. 2003; 17: 2614–2623.
5. Zadnipryanyi I.V., Tretyakova O.S., Sataeva T.P. Investigation of the antioxidant activity and cardioprotective effect of reamberin and cytoflavin in newborn rats exposed to chronic hemic hypoxia // Arkhiv patologii. 2015; 77 (6): 39–44.
6. Santangelo C., Vari R., Scazzocchio D. et al. Polyphenols, intracellular signalling and inflamation // Ann. 1st. Super. Snita. 2007; 43 (4): 394–405.
7. Tamura H., Yamagami A., Agric J. Antioxidative Activity of Monoxylated Antocyanins isolated from Muscat Bailey A Grape // Food Сhem. 1994; 42: 1612–1615.
8. Levchenkova O.S., Novikov V.E. Novyie napravleniya poiska lekarstvennyih sredstv s antigipoksicheskoy aktivnostyu i misheni dlya ih deystviya [New drugs search direction with antigipoksicheskoy activity and targets for their actions] // Eksperimentalnaya i klinicheskaya farmakologiya – Experimental and Clinical Pharmacology. 2013; 5 (76): 37–47 (in Russian).
9. Zajcev G.P., Katrich L.I., Ogaj Ju.A. Polifenol’nye biologicheski aktivnye komponenty krasnogo suhogo vinomateriala iz vinograda sorta Kaberne-Sovin’on i pishhevogo koncentrata «Еnoant» [Polyphenolic biologically active components of red dry wine from grapes of Cabernet Sauvignon and food “Enoant” concentrate] // Magarach. Vinogradarstvo i vinodelie. 2010; 3: 25–27 (in Russian).
10. Mizin V.I., Yezhov V.V., Severin N.A., Kruglova A.Y. Syndrome pathogenic approach to application of therapeutic and prophylactic effects of grape polyphenol complex under pathology of cardiovascular system // Journal of Health Sciences. 201; 3 (16): 95–108.
11. Mohiuddin, Syed M., Taskar P.K., Rheault M. et al. Experimental cobalt cardiomyopathy // American Heart Journal. 1970; 80 (4): 532–543.
12. Meshkov V.V., Bogdanov N.N., Bogdanov A.N. Eksperimentalnyie predposyilki k optimizatsii metodik primeneniya «Enoanta» [The experimental conditions to optimize the use of techniques “Enoant”] // Vestn. fizioterapii i kurortologii. – Herald Physiotherapy and Health Resort. 2002; 2: 30–33 (in Russian).
13. Merkulov G.A. Kurs patologo-gistologicheskoy tehniki [The course of pathological and histological tehniki]. L .: Meditsina Publ., 1969 (in Russian).
14. Kuo J. Electron microscopy: methods and protocols. New Jersey: Humana Press. Inc., 2007: 608.
15. Mihara M., Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test // Anal Biochem. 1978; 86 (1): 271–278.
Review
For citations:
Zadnipryany I.V., Tretiakova O.S., Kubyshkin A.V., Sataieva T.P. Protective effect of grapes polyphenol concentrate “Fenokor” in terms of hypoxic myocardial injury. Bulletin of Siberian Medicine. 2017;16(3):34-42. (In Russ.) https://doi.org/10.20538/1682-0363-2017-3-34-42
Views:
1196
Email this article 