Major salivary gland hypertrophy model in immature rats: morphometric and histochemical epithelial cell characteristics
https://doi.org/10.20538/1682-0363-2017-3-61-69
Abstract
The purpose of the study is to estimate the functional state of epithelial cells of acini and ducts of major salivary glands with hypertrophy caused by repeated incisor amputations in immature rats.
Materials and methods. The experiment was carried out on immature (20 days), white male rats, divided into 3 groups: intact, control and group of rats with repeated incisor amputations. Animals were taken out in 2d, 3d, 4th, 6th, 8th, 10th and 12th weeks after the first incisor amputation. Morphofunctional state of rat major salivary glands was assessed by histological (hematoxylin and eosin), histochemistrical (Alcian blue, PAS-reaction, Brachet method) and morphometrical (acini area, intralobular ducts volume) methods.
Results. Repeated incisor amputations led to the increase of acini area and the decrease of intralobular duct volume in submandibular glands in 2nd–4th weeks of the experiment. Cytoplasm pyroninophilia of submandibular gland acinar cells was less pronounced and intensity of PAS-reaction was more pronounced than in intact animals in 3rd week of the experiment. Morphological and functional changes of parotid and sublingual gland epithelial cells were not observed after repeated amputations of incisors in immature rats.
Conclusion. Repeated incisor amputations in immature male rats lead to submandibular gland acinar cell hypertrophy in the early stages of the experiment (2d–4th weeks) with accumulation of glycoproteins and protein synthesis weakening in these cells. Hypertrophy of acinar cells are accompanied by retardation in the development of granular convoluted tubule cells which are the source of synthesis and secretion of the endocrine biologically active factors of submandibular glands.
About the Authors
Vera V. Ivanova
Siberian State Medical University
Russian Federation
Postgraduate Student of the Department of Morphology and General Pathology, Siberian State Medical University, Tomsk, Russian Federation.
Russian Federation
Postgraduate Student of the Department of Morphology and General Pathology, Siberian State Medical University, Tomsk, Russian Federation.
Ivan V. Milto
Siberian State Medical University;
National Research Tomsk Polytechnic University
Russian Federation
DBSc, Associate Professor, Department of Morphology and General Pathology, Siberian State Medical University, Associate Professor, Department of Biotechnology and Organic Chemistry, National Research Tomsk Polytechnic University, Tomsk, Russian Federation.
Russian Federation
DBSc, Associate Professor, Department of Morphology and General Pathology, Siberian State Medical University, Associate Professor, Department of Biotechnology and Organic Chemistry, National Research Tomsk Polytechnic University, Tomsk, Russian Federation.
Irina V. Sukhodolo
Siberian State Medical University
Russian Federation
DM, Professor, Head of the Department of Morphology and General Pathology, Siberian State Medical University, Tomsk, Russian Federation.
Russian Federation
DM, Professor, Head of the Department of Morphology and General Pathology, Siberian State Medical University, Tomsk, Russian Federation.
Anna N. Dzuman
Siberian State Medical University
Russian Federation
PhD, Associate Professor, Department of Morphology and General Pathology, Siberian State Medical University, Tomsk, Russian Federation
Russian Federation
PhD, Associate Professor, Department of Morphology and General Pathology, Siberian State Medical University, Tomsk, Russian Federation
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Review
For citations:
Ivanova V.V., Milto I.V., Sukhodolo I.V., Dzuman A.N. Major salivary gland hypertrophy model in immature rats: morphometric and histochemical epithelial cell characteristics. Bulletin of Siberian Medicine. 2017;16(3):61-69. (In Russ.) https://doi.org/10.20538/1682-0363-2017-3-61-69
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