Modeling of functional ovarian cysts by introducing a follicle-stimulating hormone
https://doi.org/10.20538/1682-0363-2017-3-137-144
Abstract
Aim. To study the morphofunctional state of ovaries after the introduction of recombinant FSH.
Materials and methods. Two groups of mature female Wistar rats were used in the study. The experimental group consisted of 35 rats with a model of follicular ovarian cysts, while 25 rats were in the control group.
Rats were taken out of the experiment on days 3, 7, 15, 30 and 60. Paraffin slides of ovaries were stained with hematoxylin and eosin by Van Gieson. Histological and morphometric investigations were performed.
Results. On day 7 of FSH administration, the maximum increase in the size of the ovaries was observed due to the formation of single-cell follicular cysts. Rats in the experimental group showed a marked decrease in the number of growing follicles on day 7 and 15. The increase in atretic bodies and follicles in comparison with the control group was observed on day 7 and lasted until the end of the experiment. Additionally, on day 7 of the experiment, hyperemia and vasoconstriction were noted. The number of yellow bodies decreased during the experiment, and an increase in collagen formation occurred starting from day 15 of the experiment.
Conclusion. The introduction of follicle-stimulating hormone for 7 days leads to disruption of folliculogenesis, strengthening of atresia in the ovaries, and the formation of functional cysts.
About the Authors
Daria A. Trus
Siberian State Medical University (SSMU)
Russian Federation
Postgraduate Student, Department of Histology, Embryology and Cytology, SSMU, Tomsk, Russian Federation.
Russian Federation
Postgraduate Student, Department of Histology, Embryology and Cytology, SSMU, Tomsk, Russian Federation.
Olga A. Tikhonovskaya
Siberian State Medical University (SSMU)
Russian Federation
DM, Professor, Department of Obstetrics and Gynecology, SSMU, Tomsk, Russian Federation.
Russian Federation
DM, Professor, Department of Obstetrics and Gynecology, SSMU, Tomsk, Russian Federation.
Aleksandr O. Okorokov
Siberian State Medical University (SSMU)
Russian Federation
PhD, Assistant, Department of Obstetrics and Gynecology, SSMU,Tomsk, Russian Federation.
Russian Federation
PhD, Assistant, Department of Obstetrics and Gynecology, SSMU,Tomsk, Russian Federation.
Liliya R. Mustafina
Siberian State Medical University (SSMU)
Russian Federation
DМ, Professor, Department of Histology, Embryology and Cytology, SSMU,Tomsk, Russian Federation.
Russian Federation
DМ, Professor, Department of Histology, Embryology and Cytology, SSMU,Tomsk, Russian Federation.
O. S. Timofeeva
Siberian State Medical University (SSMU)
Russian Federation
Resident, Department of Obstetrics and Gynecology, SSMU, Tomsk, Russian Federation.
Russian Federation
Resident, Department of Obstetrics and Gynecology, SSMU, Tomsk, Russian Federation.
Sergey V. Logvinov
Siberian State Medical University (SSMU)
Russian Federation
DM, Professor, Head of the Department of Histology, Embryology and Cytology, SSMU, Tomsk, Russian Federation.
Russian Federation
DM, Professor, Head of the Department of Histology, Embryology and Cytology, SSMU, Tomsk, Russian Federation.
Alexandr V. Gerasimov
Siberian State Medical University (SSMU)
Russian Federation
DМ, Professor, Department of Histology, Embryology and Cytology, SSMU, Tomsk, Russian Federation.
Russian Federation
DМ, Professor, Department of Histology, Embryology and Cytology, SSMU, Tomsk, Russian Federation.
Elena Yu. Varakuta
Siberian State Medical University (SSMU)
Russian Federation
DМ, Professor, Department of Histology, Embryology and Cytology, SSMU,Tomsk, Russian Federation.
Russian Federation
DМ, Professor, Department of Histology, Embryology and Cytology, SSMU,Tomsk, Russian Federation.
Aleksey V. Potapov
Siberian State Medical University (SSMU)
Russian Federation
DМ, Professor, Department of Histology, Embryology and Cytology, SSMU,Tomsk, Russian Federation.
Russian Federation
DМ, Professor, Department of Histology, Embryology and Cytology, SSMU,Tomsk, Russian Federation.
Galina A. Suhanova
Siberian State Medical University (SSMU)
Russian Federation
DBSc, Professor, Department of Biochemistry, SSMU, Tomsk, Russian Federation.
Russian Federation
DBSc, Professor, Department of Biochemistry, SSMU, Tomsk, Russian Federation.
Irina G. Kutsenko
Siberian State Medical University (SSMU)
Russian Federation
DM, Professor, Department of Obstetrics and Gynecology, SSMU, Tomsk, Russian Federation.
Russian Federation
DM, Professor, Department of Obstetrics and Gynecology, SSMU, Tomsk, Russian Federation.
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Review
For citations:
Trus D.A., Tikhonovskaya O.A., Okorokov A.O., Mustafina L.R., Timofeeva O.S., Logvinov S.V., Gerasimov A.V., Varakuta E.Yu., Potapov A.V., Suhanova G.A., Kutsenko I.G. Modeling of functional ovarian cysts by introducing a follicle-stimulating hormone. Bulletin of Siberian Medicine. 2017;16(3):137-144. (In Russ.) https://doi.org/10.20538/1682-0363-2017-3-137-144
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