EFFECT OF SILVER NANOPARTICLES IN EXPERIMENTAL PERITONITIS
https://doi.org/10.20538/1682-0363-2015-2-67-74
Abstract
In our research we have investigated the effect of intraperitoneal introduction of silver nanoparticles solution (linear particle size of 10–20 nm) into intact animals and in the simulation of experimental peritonitis. We have evaluated the indices of nonspecific proteinases and their inhibitors in blood serum and peritoneum lavage. The intraperitoneal introduction of silver nanoparticles solution to intact animals leads to the minimal reaction of proteinase-inhibitor systems components in blood serum and peritoneum lavage. When modeling inflammatory process in peritoneum cavity by intraperitoneal injection of 10% filtered fecal suspension of rats, application of silver nanoparticles solution is accompanied by decrease in extent of activation of proteinases and preservation of inhibitory potential, both at systemic and local level, which can indicate antiinflammatory effects of nanosilver.
About the Authors
D. V. Chegodar
Crimea State Medical University named after S.I. Georgievsky, Simferopol
Russian Federation
Russian Federation
A. V. Kubyshkin
Crimea State Medical University named after S.I. Georgievsky, Simferopol
Russian Federation
Russian Federation
V. V. Panasenko
Center of hygiene and epidemiology in the Crimea Republic and federal city of Sevastopol, Simferopol
Russian Federation
Russian Federation
References
1. Zhang L., Gu F.X., Chan J.M., Wang A.Z., Langer R.S., Farokhzad O.C. Nanoparticles in medicine: therapeutic applications and developments. Clin. Pharmacol. Ther., 2008, vol. 83, no. 5, pp. 761–769.
2. Chen X., Schlusener H.J. Nanosilver: nanoproduct in medical application. Toxicol. Lett., 2008, vol. 176, no. 1, pp. 1–12.
3. Chekman I.S., Movchan B.A., Zagorodny M.I., Gaponov Yu.V., Kurapov Yu.A., Krushinskaya L.A., Kardash M.V. Nanoserebro: tehnologii poluchenija, farmakologicheskie svojstva, pokazanija k primeneniju [Nanosilver: technologies of production, pharmacological properties, indications to application]. Iskusstvo lechenija, 2008, no. 5, pp. 32–34.
4. Chekman I.S., Priskoka A.O., Babij V.F., Antonenko O.V., Zagorodny M.I. Medicinskoe primenenie nanochastic serebra: toksikologicheskij aspekt [Medical applications of silver nanoparticles: toxicological aspect]. Sovremennye problemy toksikologii, 2010, no. 4, pp. 10–13.
5. Bidgoli S.A., Mahdavi M., Rezayat S.M., Korani M., Amani A., Ziarati P. Toxicity Assessment of Nanosilver Wound Dressing in Wistar Rat. Acta Medica Iranica, 2013, vol. 51, no. 4, pp. 203–208.
6. Cheraghi J., Hosseini E., Hoshmandfar R., Reza Sahraei, Abbas Farmany. In vivo effect of Silver Nanoparticles on serum ALT, AST and ALP activity in male and female mice. Adv. Environ. Biol., 2013, vol. 7, no. 1, pp. 116–122.
7. Sukovatykh B.S., Blinkov Yu.Yu., Frolova O.G. Mehanizmy razvitija rasprostranennogo peritonita [Mechanisms of the diffuse peritonitis development]. Vestnik jeksperimental'noj i klinicheskoj hirurgii – Bulletin of Experimental and Clinical Surgery, 2012, vol. 5, no. 2, pp. 470–478. 8. Yermola Yu.A., Fomochkina I.I., Kubyshkin A.V. Lokal'nye i sistemnye izmenenija pokazatelej nespecificheskih proteinaz i ih ingibitorov pri jeksperimental'nom peritonite [Local and systemic changes of nonspecific proteases and their inhibitors in experimental peritonitis]. Ukrainskij medicinskij al'manah, 2012, vol. 15, no. 5, pp. 80–82.
8. Babanin A.A., Kubyshkin A.V., Ermola Yu.A., Chegodar D.V., Yurkova I.N., Fedosov M.I. Changes of nonspecific proteinases and free-radical oxidation processes in treatment of experimental peritonitis with the nanobiosilver solution. The New Armenian Medical Journal, 2014, vol. 8, no. 1, pp. 46–51.
9. Lazarenko V.A., Lipatov V.A., Blinkov Yu.Yu., Skorikov D.V. Jeksperimental'naja model' rasprostranennogo kalovogo peritonita [Experimental model of diffuse fecal peritonitis]. Kurskij nauchno-prakticheskij vestnik "Chelovek i ego zdorov'e" – Kurskiy Scientifically-Practical Herald “Persons and his health”, 2008, no. 4, pp. 128–132.
10. Yurkova I.M., Estrella-Lopys V.R., Ryabushko V.I., Ryabushko L.I. The patent of the invention no 10539, MCI 7
11. A61K33/38, A61K31/715. A method for producing a watersoluble bactericidal composition comprising silver nanoparticles. No 200500475; Appl. 13.05.05; publ. 15.11.05; Bulletin no. 11. (in Russia).
12. Kubyshkin A.V., Kharchenko V.Z., Semenec P.F., Aliev L.L., Fomochkina I.I., Anisimova L.V. Metody opredelenija aktivnosti nespecificheskih proteinaz i ih ingibitorov v syvorotke krovi i biologicheskih zhidkostjah (Metodicheskie rekomendacii) [Methods of determination of the nonspecific proteinases and their inhibitors activity in blood serum and biological fluids (Methodical recommendation)]. Kiev, 2010. 28 p.
13. Chekman I.S. Nanofarmakologija: vzgljad na problem [Nanopharmacology: view at the problem]. Vestnik NAN Ukrainy, 2012, no. 7, pp. 21–25.
14. Rybachuk A.V., Chekman I.S. Protivomikrobnye svojstva nanoserebra [Antimicrobial properties of nanosilver]. Ukrainskij nauchno-medicinskij molodezhnyj zhurnal – The Ukrainian scientifically-medical youth magazine, 2009, no. 2, pp. 32–36.
15. Melaiye A., Sun Z., Hindi K., Milsted A., Ely D., Reneker D.H., Tessier C.A., Youngs W.J. Silver(I)-imidazole cyclophane gem-diol complexes encapsulated by electrospun tecophilic nanofibers: formation of nanosilver particles and antimicrobial activity. J. Am. Chem. Soc., 2005, vol. 127, no. 7, pp. 2285–2291.
16. Egorova E.M., Kubatiev A.A., Shvec V.I. Biologicheskie jeffekty nanochastic metallov [Biological effects of metal nanoparticles]. Moscow, Nauka Publ., 2014, 350 p.
Review
For citations:
Chegodar D.V., Kubyshkin A.V., Panasenko V.V. EFFECT OF SILVER NANOPARTICLES IN EXPERIMENTAL PERITONITIS. Bulletin of Siberian Medicine. 2015;14(2):67-74. (In Russ.) https://doi.org/10.20538/1682-0363-2015-2-67-74
Views:
891
Email this article 