Spontaneous apoptosis of lymphocytes of ovary cancer patients
https://doi.org/10.20538/1682-0363-2007-2-8-10
Abstract
Results of the study of the spontaneous apoptosis mechanisms of peripheral blood lymphocytes of ovary cancer (OC) patients are presented in the article. Dynamics of mitochondrial potential (MP) change, level of phosphatidylserine (PS) expression and DNA fragmentation were used as apoptosis markers. These parameters were estimated by flow cytometry. During the incubation process, DNA fragmentation in OC patient lymphocytes were more intensive and observed during earlier stages than those in donor lymphocytes. Also, reliable decrease of MP and increase of PS expression level were observed. The study results revealed increased reactivity of mitochondrial lymphocytes in OC patients and as a result, decreased resistance to spontaneous apoptosis processes.
About the Authors
I. I. Antoneyeva
Ульяновский государственный университет, г. Ульяновск
Russian Federation
Russian Federation
S. V. Boichuk
Казанский государственный медицинский университет, г. Казань
Russian Federation
Russian Federation
References
1. Билынский Б.Т., Володько Н.А., Шпарык Я.В. Иммунологические механизмы естественной противоопухолевой резистентности человека. Львов, 1989. 64 с.
2. Новиков В.С. Программированная клеточная гибель. СПб., 1996.
3. Castedo M., Hirsch T., Susin S. et al. Seguential asguisition of mitochondrial and plasma membrane alterations during larly lymphocyte apoptosis // J. Jmmunol. Muth. 1996. V. 157. P. 512-521.
4. Macho A., Decandin D., Cartedo M. et al. Chloromethyl-XRosamine is an aldehydefixable potential-sensitive fluorochrome for the detection of larly apoptosis // Cytometry. 1996. V. 25. P. 333 -340.
5. Martin S.J., Brein C.A., Nishiora W.K. et al. Proteolysis of fodrin (non Erythroid spectrin) during apoptosis // J. Biol. Chem. 1995. V. 270. P. 6425-6429.
6. Mower D.A., Peckhman D.W., Jelera V.A. et al. Decreased membrane phospholipids packing and decreased cell size precede DNA cleavage in maturu mouse B cell apoptosis // J. Immunol. 1994. V. 152. P. 4832-4842.
7. Nicoletti J., Migliorati J. Rapid and simple method of measurement of nuclear apoptosis // J. Immunol. Muth. 1991. V. 139. P. 271- 279.
8. Phehn R.T. The immune reaction as a stimulator of tumor growth // Science. 1972. V. 176. P. 170-171.
Review
For citations:
Antoneyeva I.I., Boichuk S.V. Spontaneous apoptosis of lymphocytes of ovary cancer patients. Bulletin of Siberian Medicine. 2007;6(2):8-10. (In Russ.) https://doi.org/10.20538/1682-0363-2007-2-8-10
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