Content of bone morphogenetic proteins in blood plasma of rats with metabolic syndrome during mandibular defect restoration
https://doi.org/10.20538/1682-0363-2026-2-65-72
Abstract
The aim was to study the concentration of bone morphogenetic proteins-2 (BMP) and -7 in the blood plasma of rats with metabolic syndrome during reparative osteogenesis in mandibular defect.
Materials and methods. The experiments were carried out on male Wistar rats, which were divided into 4 groups: 1) naïve; 2) control (with mandibular defect); 3) metabolic syndrome; 4) experimental (with metabolic syndrome and mandibular defect). Metabolic syndrome was simulated by replacing standard feed with a high-fat and highcarbohydrate diet. The development of metabolic syndrome was confirmed by changes in body weight, glucose and triacylglycerides concentrations in blood plasma. The mandibular defect was formed in rats according to the method proposed by Grigoryan et al. The content of BMP-2 and BMP-7 in blood plasma was determined by the enzyme-linked immunoassay on days 7, 14, 21, and 28 after the surgery. We calculated the median and the interquartile range Me [Q1; Q3] and applied the Mann – Whitney U-test and the Bonferroni correction. Differences in indicators were considered significant at p < 0.025.
Results. A significant increase in the content of the studied proteins in the blood plasma was determined in the group metabolic syndrome compared to the naïve group. BMP-2 and BMP-7 concentration in rats of the control group was higher throughout the experiment compared to the naïve group (p < 0.025). In the experimental group, a decrease in the concentration of BMP-2 (on days 7, 14, and 28) and BMP-7 (on days 7–28) was found compared to the control group (p < 0.025). An increase in the concentration of BMP-2 and BMP-7 was detected in the experimental group compared to the metabolic syndrome group (p < 0.025) on day 7 and on days 7–14, respectively.
Conclusion. A significant decrease in BMP-2 and BMP-7 concentration in the blood plasma was found in rats with metabolic syndrome during mandibular reparative osteogenesis compared to animals without metabolic disorders.
About the Authors
M. V. Makarova
Kursk State Medical University (KSMU)
Russian Federation
Russian Federation
3 Karl Marx St., 305041 Kursk, Russian Federation
V. N. Tsygan
Kirov Military Medical Academy
Russian Federation
Russian Federation
6g Academician Lebedev St., 194044 Saint-Petersburg, Russian Federation
Yu. D. Lyashev
Kursk State Medical University (KSMU)
Russian Federation
Russian Federation
3 Karl Marx St., 305041 Kursk, Russian Federation
A. Ye. Brusentsova
Kursk State Medical University (KSMU)
Russian Federation
Russian Federation
3 Karl Marx St., 305041 Kursk, Russian Federation
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Review
For citations:
Makarova M.V., Tsygan V.N., Lyashev Yu.D., Brusentsova A.Ye. Content of bone morphogenetic proteins in blood plasma of rats with metabolic syndrome during mandibular defect restoration. Bulletin of Siberian Medicine. 2026;25(2):65-72. https://doi.org/10.20538/1682-0363-2026-2-65-72
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