The influence of preanalytical factors on the algorithm of multiplex spectroscopy of placental samples
V. E. Rodionov,
A. M. Avdalyan,
A. I. Nichiporov,
I. A. Chernov,
V. I. Kukushkin,
S. T. Avraamova,
Yu. A. Kirillov https://doi.org/10.20538/1682-0363-2026-2-84-90
Abstract
Aim. To evaluate the effect of preanalytical processing of placental samples on the results of Raman spectroscopy.
Materials and methods. Thirty placental samples were prospectively studied. The samples were examined using the InSpectr M spectrometer at three stages of preanalytical processing of the material: native tissue, after fixation in formalin, after deparaffinization of the section. Statistical analysis included descriptive statistics, the Shapiro – Wilk test, the Friedman test with the post-hoc analysis, and the Spearman’s rank correlation.
Results. The analysis of the samples revealed 33 Raman peaks (415–2,915 cm–1), most of which were distributed abnormally (Shapiro – Wilk test, p < 0.05). The Friedman test with the Durbin – Conover post-hoc analysis revealed a group of stable analytes: 19 analytes retained intensity after fixation, 6 – after deparaffinization, and 10 – at all processing stages. The Spearman’s rank correlation revealed selective relationships between processing stages for individual analytes (890; 1,117; 1,204; 753; 830, and 2,225 cm–1).
Conclusion. The study revealed a significant effect of preanalytical sample processing on the spectral characteristics of placental tissue, the prospects for the clinical use of Raman spectroscopy, and the need to develop standardized protocols.
About the Authors
V. E. Rodionov
Moscow Multidisciplinary Clinical Center “Kommunarka”;
Tyumen State Medical University
Russian Federation
Russian Federation
8/3 Sosensky Stan St., 108814 Kommunarka village, Moscow, Russian Federation
54 Odesskaya St., 625023 Tyumen, Russian Federation
A. M. Avdalyan
Moscow Multidisciplinary Clinical Center “Kommunarka”
Russian Federation
Russian Federation
8/3 Sosensky Stan St., 108814 Kommunarka village, Moscow, Russian Federation
A. I. Nichiporov
Moscow Multidisciplinary Clinical Center “Kommunarka”
Russian Federation
Russian Federation
8/3 Sosensky Stan St., 108814 Kommunarka village, Moscow, Russian Federation
I. A. Chernov
Tyumen State Medical University
Russian Federation
Russian Federation
54 Odesskaya St., 625023 Tyumen, Russian Federation
V. I. Kukushkin
Institute of Solid State Physics, Russian Academy of Sciences
Russian Federation
Russian Federation
2 Academician Osipyan St., 142432 Chernogolovka, Moscow Region, Russian Federation
S. T. Avraamova
The First Sechenov Moscow State Medical University
Russian Federation
Russian Federation
8/2 Trubetskaya St., 119992 Moscow, Russian Federation
Yu. A. Kirillov
Moscow Multidisciplinary Clinical Center “Kommunarka”;
Tyumen State Medical University;
Avtsyn Research Institute of Human Morphology, Petrovsky National Research Centre of Surgery
Russian Federation
Russian Federation
8/3 Sosensky Stan St., 108814 Kommunarka village, Moscow, Russian Federation
54 Odesskaya St., 625023 Tyumen, Russian Federation
3 Tsyurupy St., 117418 Moscow, Russian Federation
References
1. Громова О.А., Торшин И.Ю., Чучалин А.Г., Лазебник Л.Б. Ферритин как биомаркер полиорганной патологии: противовоспалительные и антиоксидантные пептиды стандартизированного гидролизата плаценты человека. Экспериментальная и клиническая гастроэнтерология. 2024;(8):113–122. DOI: 10.31146/1682-8658-ecg-228-8-113-122.
2. Чернышова А.Л., Черняков А.А., Трущук Ю.М., Юнусова Н.В., Севостьянова Н.В., Архипова Я.И. и др. Молекулярно-генетический и биохимический профили злокачественных новообразований на фоне беременности. Успехи молекулярной онкологии. 2024;11(3):32–40. DOI: 10.17650/2313-805X-2024-11-3-32-40.
3. Zhuang Z., Zhu M., Huang Y., Liu J., Guo Z., Xiong K. et al. Study of molecule variation in various grades of human nuclear cataracts by confocal micro-Raman spectroscopy. Appl. Phys. Lett. 2012;101(17):173701. DOI: 10.1063/1.4762838.
4. Zhuang Z., Li N., Guo Z., Zhu M., Xiong K., Chen S. Study of molecule variations in renal tumor based on confocal micro-Raman spectroscopy. J. Biomed. Opt. 2012;18(3):031103. DOI: 10.1117/1.JBO.18.3.031103.
5. Zhuo S., Chen J., Wu G., Xie S., Zheng L., Jiang X. et al. Quantitatively linking collagen alteration and epithelial tumor progression by second harmonic generation microscopy. Appl. Phys. Lett. 2010;96(21):213704. DOI: 10.1063/1.3441337.
6. Zheng-Fei Z., Han-Ping L., Zhou-Yi G., Shuang-Mu Z., BiYing Y., Xiao-Yuan D. Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells. Chinese Physics B. 2010;19(4):049501. DOI: 10.1088/1674-1056/19/4/049501.
7. Chen S.J., Zhang Y., Ye X.P., Hu K., Zhu M.F., Huang Y.Y. et al. Study of the molecular variation in pre-eclampsia placenta based on micro-Raman spectroscopy. Archives of Gynecology and Obstetrics. 2014;290(5):943–946. DOI: 10.1007/s00404-014-3282-9.
8. Pielesz A., Bobiński R., Biniaś D., Gawłowski A., Waksmańska W., Ulman-Włodarz I. et al. FT Raman spectroscopy in the evaluation of biomarkers of normal and pathological placenta tissue. Molecular and Cellular Biochemistry. 2019;458(1):125–132. DOI: 10.1007/s11010-019-03536-5.
9. Başar G., Parlatan U., Şeninak Ş., Günel T., Benian A., Kalelioğlu İ. Investigation of preeclampsia using raman spectroscopy. Journal of Spectroscopy. 2012;27(4):376793. DOI: 10.1155/2012/376793.
10. Monaghan J.F., Cullen D., Wynne C., Lyng F.M., Meade A.D. Effect of pre-analytical variables on Raman and FTIR spectral content of lymphocytes. Analyst. 2023;148(21):5422–5434. DOI: 10.1039/D3AN00686G.
11. Bocklitz T., Walter A., Hartmann K., Rösch P., Popp J. How to pre-process Raman spectra for reliable and stable models? Analytica Chimica Acta. 2011;704(1):47–56. DOI: 10.1016/j.aca.2011.06.043.
12. Campanella B., Legnaioli S., Onor M., Benedetti E., Bramanti E. The role of the preanalytical step for human saliva analysis via vibrational spectroscopy. Metabolites. 2023;13(3):393. DOI: 10.3390/metabo13030393.
13. Movasaghi Z., Rehman, S., Rehman Ihtesham U. Raman spectroscopy of biological tissues. Applied Spectroscopy Reviews. 2007;42(5):493–541. DOI: 10.1080/05704920701551530.
14. Roy A., Mantay M., Brannan C., Griffiths S. Placental tissues as biomaterials in regenerative medicine. BioMed Research International. 2022;2022(1):6751456. DOI: 10.1155/2022/6751456.
15. Howat W.J., Wilson B.A. Tissue fixation and the effect of molecular fixatives on downstream staining procedures. Methods. 2014;70(1):12–19. DOI: 10.1016/j.ymeth.2014.01.022.
16. Alturkistani H., Faris T., Zuhair M. Histological Stains: A Literature Review and Case Study. Global Journal of Health Science. 2015;8:72–72. DOI: 10.5539/gjhs.v8n3p72.
Review
For citations:
Rodionov V.E., Avdalyan A.M., Nichiporov A.I., Chernov I.A., Kukushkin V.I., Avraamova S.T., Kirillov Yu.A. The influence of preanalytical factors on the algorithm of multiplex spectroscopy of placental samples. Bulletin of Siberian Medicine. 2026;25(2):84-90. https://doi.org/10.20538/1682-0363-2026-2-84-90
Views:
57
JATS XML
Email this article 