The results of study, in which we examined the influence of nanosized magnetite on breath organs histological structure and contractility activity of the airways of guinea pigs by method of mechanography has been presented. In the lungs of experimental animals an inflammatory response developed as a result of long-term inhalation intake of nanosized magnetite. Also the functional status of the airways changed and appeared as changing of amplitude of contractility response under the action of histamine.

120 patients were examined using instrumental methods, such as hemocoagulography and agregatography. It has been various methods of postoperative anesthesia have different effect on the functional status of haemostasis system following hip replacement. Optimization of postoperative anesthesia provides a way of preventing thrombohemorrhagic complications

 

Features of brain bioelectric activity and thyroid system in adolescents living in Subpolar andPolar regionsof the North are considered. Hyperactivity of subcortical diencephalic brain structures in adolescents of the Polar region is revealed. Adolescents of Subpolar region have more intensive age optimization of neurodynamic processes. There are noted latitude distinctions of thyroid hormones role for age formation of brain bioelectric activity in adolescents.

The function of endothelium of microcirculation vessels, vegetative and psychoemotional status, lipid spectrum and physical efficiency in 43 young sportsmen are corrected with the aid of Tentorium api-phyto products (APP).

The following tests were conducted: samples of reactive hyperemia and hyperventilation, ultrasound investigation of brachial artery, determination of performance efficiency, total cholesterin, triglycerides, cortisol, red cells, vegetative and psychoemotional status by the Spielberger’s test and SAN test, as well as biomicroscopy of sclera. To relief the psychophysical stress, improve the endothelium function and performance efficiency, the sportsmen received APP as a food additive.

After the reception of APP, the lipid and cortisol levels decreased markedly, and the level of red cells increased. The aerobic capacity increased. The recovery time after exercise shortened. The vegetative stress level decreased significantly, and the psychoemotional status improved. The endothelium dysfunction removed.

It is shown that APP is a new method of prophylaxis of sudden death in sport, in people with blood circulation pathology, creative professionals, and businessmen.

A method of local adrenal organ-preserving cryolysis using a porous nickelide titanium cryoprobe created for this purpose was developed in experiments in 14 dogs. The efficacy of the method was demonstrated by the morphological evidence and hormone tests.

Leucomisine model of acute hyperlipidemia in rats induced by administration of ethanol reduces the levels of free fatty acids and triacylglycerols in serum and the content of triacylglycerols in the liver of experimental animals. Introduction sesquiterpenoid leucomisine increases the activity of enzymes antiperoxidant protecti- on – glutathione peroxidase and glutathione reductase and the level of total and reduced glutathione, which prevents the activation of lipid peroxidation in the liver caused by ethanol. Lipid-lowering effect induced by the hyperlipidemia leucomisine when, like nicotinic acid, may be due to an inhibitory effect on lipolysis in adipose tissue. Along with this leucomisine has an antioxidant effect due to the activation of enzymes antiperoxidant protect and increase the redox potential of glutathione in the liver of rats. 

The study has demonstrated that proapoptic concentrations of donors of NO (100 mmol SNP and 100 µmol NOC-5), H₂S  (10 mmol NaHS), and CO (50 µmol CORM-2) gases caused an increase in the intracellular level of active forms of oxygen in Jurkat cells. As this took place, the activation of redox-dependent transcription factor р53 was observed as Jurkat cells were exposed to 100 mmol SNP and 10 mmol NaHS. In the case of 100 µmol NOC-5 and 50 µmol CORM-2, an increase of р53 was not observed, but the expression of target gens of this transcription factor р21 (under the effect of NO and СО) and bax (under the effect of NO) increased. The antiproliferative concentration of hydrogen sulfide donor (50 µmol ) did not cause an increase in the intracellular production of active forms of oxygen and the activation of redox-dependent signal mechanisms.

The wide application of cardiac surgery using the aorto-pulmonary bypass technique needs the development of new drugs, capable to minimize the heart damaging during ischemia and reperfusion. But the direct delivery of drugs into the damaged tissues and cells is a big problem. The liposomes as unique transport system can be used to solve this problem.

The aim of the study was to evaluate the influence of "empty" liposomes of various diameter and the liposomes of different composition on the myocardium contractile function which has underwent the ischemia and the subsequent reperfusion. The obtained results allowed us to make the conclusion that liposomes of 50 nanometers in diameter are most effective to cardiac protection versus liposomes of 100 nanometers.

Inclusion the emoxipine into the composition of liposomes provide the best results on contractile function of an ischemic myocardium.

Local and system influence the Repleri gel implants on the base of hyaluronic acid used to correct age-related changes in skin has been studied. Human stromal stem cells with CD44 membrane receptor to hyaluronic acid migrates in vitro into gel structure and maturates morphologically in fibroblast-like forms stained positively with acid phosphatase. Repleri gels implantation in the group of women with local and system age-related changes in homeostasis has positive local effect connected with full deletion of nasolabial fold in facial skin. Repleri gels injection has been decreasing during 
1 year the system indices of real biological age as compared with chronological (passport) one. Lipoproteins and aspartate aminotransferase concentrations diminished statistically in women blood. An effect revealed may be considered as potential way of anti-age therapy of not only skin but parenchymal organs. At the same time, a long-term control of homeostasis distant indices in women with serious changes in parenchymal organs is necessary after local Repleri gels injection because of organism’s hormonal reactions.

The resistance of red cells to the mechanic, osmotic, and acid factors, as well as the permeability of red-cell membrane to low-molecular hydrophilic substances in patients with ischemic heart disease (IHD) with moderate (24 patients) and pronounced (13 patients) hemolysis after surgery under conditions of cardia bypass has been studied. It is found that the development of pronounced hemolysis (in contrast to the moderate one) is associated with the high permeability of the red-cell membrane before surgery at the reduced acid and mechanic resistance of cells, which decreases after surgery and combines with normalization of acid resistance of red cells with the red-cell membrane permeability kept at the initially high level. The osmotic resistance of red cells in IHD patients is increased regardless of the level of hemolysis and the stage of the study, and its minimal level is observed only in patients with moderate hemolysis before surgery.

The composition of lipid phase of red-cell membrane in patients with ischemic heart disease (IHD) with pronounced postperfusion hemolysis (18 patients) before coronary artery bypass surgery and 1 hour after the completion of cardia bypass (CB) has been studied. It is shown that patients with IHD with pronounced hemolysis are characterized by the normal ratio of phospholipids (PL) fractions in red-cell membrane before surgery, which is connected with eth high content of young forms of red cells in blood at this stage. After surgery, the fraction of lysophosphatidylcholine and phosphatidic acid in red-cell membrane increases against the background of decrease of phosphatidylinositol  and phosphatidylcholine, which likely reflects the simultaneous activation of phospholipases of three classes (A, C, and D) in red cells during CB. Regardless of the phase of the study, the total content of PL in red-cell membrane of IHD patients with pronounced hemolysis is decrease at the high level of cholesterol (CS) and the CS/Pl ratio.

In experimental liver pathology caused by isoniazide or paracetamol administration to albino rats lipids derived from maral antlers and peat decreased the blood activity of aminotransferases, γ-glutamyltranspeptidase, acid and alkaline phosphatases, phospholipase А, content of common bilirubin, activated the detoxication of biliribin, ammonium and phenols, inhibited the liver formation of dienic conjugates, Schiff’s bases, malonic dialdehyde, improve the reduced glutathione function. Maral anthlers and peat lipids in effective doses 30 and 60 mg/kg had the more pronounced hepatoprotective and antioxidant action than lipids in dose 10 mg/kg and essentiale forte N.

Literature review takes a look at the correlation of widespread disease – obesity and respiratory function. Obesity gives a complex effect on all organs and systems of organism. In recent years has been a lot of research conducted to assess the impact of obesity on lung function. Summarizing the results of the con-ducted research at the moment, we can conclude that obesity is definitely affects the respiratory function, including patients with broncho-pulmonary disease, worsening of their symptoms. 

In the review the current understanding of some clinically and pathogenetically meaningful features of the lipid and carbohydrate metabolism in the indigenous and migrant population of the North and equivalent areas of Siberia are stated. Physiological and pathophysiological importance of the changes in human metabolism in the North are discussed in connection with the processes of adaptation and disadaptation to extreme climatic and geographical factors.

Diabetic neuropathy is the most common complications of diabetes and is a leading cause of disability of patients. According to epidemiological studies, depending on the methods of examination frequency of diabetic neuropathy among the patients ranged from 5 to 100%. The disease significantly reduces the quality of life of patients and is one of the major risk factors for foot ulcers, burns, frostbite, gangrene and diabetic foot syndrome.

The main objective of this publication – to make a review of the literature in recent years to a wider circle of local doctors with the most modern methods of early diagnosis of diabetic neuropathy.

The aim of the clinical test was to develop a method for combined rehabilitation of patients after endoscopic cholecystectomy at the early stage with the use of magnetic-laser therapy, UHF therapy, and mineral waters.

The analysis of obtained results has indicated that the combined rehabilitation of cholelithiasis patients after endoscopic cholecystectomy with the use of natural and и preformed physical factors favored the improvement of indices of functional activity of the hepatobiliary system, psychoemotional state, and adaptability of the organism, not causing their stress.

The aim is to report a rare observation of pseudotumors of small bowel leading to chronic invagination and recidivating small bowel obstruction.

The case is interesting because the information about pseudotumors of small bowel is unavailable in the literature, whereas in large bowel they are quite frequently observed, and their number is a tendency to increase. A complication of pseudoturmors is a chronic invagination  of small bowel, which is also rarely observed (in 9.05–9.58%). Its clinical picture is subtle, causing difficulties in diagnosis. In the reported observation, the diagnosis “chronic invagination” was also made only at surgery, while the diagnosis “pseudoturmor” was made after histologic examination of the specimen.

For well-timed diagnosis, in the presence of clinical findings of recidivating small bowel obstruction, chronic invagination should be considered as one of the possible causes of obstruction.

During studying of opinion of physicians and heads of Tomsk oblast federal healthcare institutions' departments it was revealed that stuff of institutions have a positive attitude to current changes only in case of being informed enough about territorial program of state guarantees of providing of free medical care to population and about main directions of reforms. On the basis of received results of survey suggestions were formed, which are directed to improving the awareness of employees and formation of personnel policy of institutions.

The material for the study included patients with chronic obstructive pulmonary disease (COPD) in association with arterial hypertension (AH) II stage – 50 people. A comprehensive clinical and functional study of the assessment of emotional status. Found that with the increase of the severity of COPD and hypertension increases the frequency and severity of cognitive impairment up to dementia.

The creative biography of the famous Russian scientist – Honored Scientist of Russia, MD, Director (1990 to 2011), chief scientific officer of the Tomsk Research Institute of Balneology and Physiotherapy FMBA of Russia, Head (1995 to 2012) of Chair of Rehabilitation Medicine, Physiotherapy and Health Resort of Siberian State Medical University (Tomsk), Professor Ye.F. Levitsky is presented. The main trends of scientific and social activities of the scientist are concerned.

Epidemiology situation and factors its determinates was analyzed. Reports of tuberculosis hospitals used and results of observed two hundred and sixty-three children with tuberculosis. It was determinate that reasons of increase children morbidity of tuberculosis: bad epidemiology situation in adults in region, not all adult patients with tuberculosis was hospitalized, defects of prophylactic of tuberculosis. Tuberculosis in children having contact with tuberculosis was determinates earlier and structure of clinical forms was better than in children which have no contact.

The composition and effectiveness of the treatment of patients 159 drug-resistant TB of Mycobacterium tuberculosis. Dominated by common processes (87%), massive bacterioexcretion (74.2%) and multidrug-resistant TB. Full clinical effect achieved in 54.8% (n = 68), including 28.8% (37) by surgical methods. 

The aim of the work is to find an optimal combination of treatment-and-health-improving activities for the preventive treatment. There are four groups of children with the different levels of the risk: very high, high, medium and low. The most important factors of the morbidity are the following: a contact with a bacteria-excreting person and with MDR, social disadvantage, hyperergic reactions. An essential condition of an effective prevention is a realization of the treatment-and-health-improving activities for the strengthening of the resistance of the body for the tuberculosis infection. The chemoprophylaxis had been realizing for 3 or 6 months according to the following developed modes: “0A”, “0Б”, “0B”. Children from the family contact with MDR got isoniazid 15 mg/kg weight and ethambutol or pyrazinamide. 

The difficulties in the interpretation of identified during bronchoscopy local bronchi alterations in patients with pulmonary tuberculosis make this problem of current interest. The comprehensive survey of 287 patients revealed the specific origin of local alterations of bronchial tree in 50% of patients with destruc-tive pulmonary tuberculosis and with bacterioexcretion

We have investigated dendritic cells (DC), obtained by transformation from peripheral blood monocytes. We show the possible mechanisms of infringement of dendritic cells (DC) functional activity in patients with pulmonary tuberculosis (TB). Found that in patients with TB the number of DC, expressing TLR2, costimulation molecules CD86, is reduced, secretion of cytokine IL-12 changes. It is possible the mecha-nisms of deactivation DC mediated from effects of M. tuberculosis 

The work analyzed subpopulation structure of peripheral blood T-regulatory cells (T-reg) and production of cytokines with immunosuppressor activity (IL-10, TGF-b) in vitro in patients with disseminated pulmonary tuberculosis, depending on the sensitivity of Mycobacterium tuberculosis to anti-tuberculosis drugs. It has been shown that Trn – natural T-regulatory lymphocytes (CD4+CD25+Foxр3+) and TGF-b cytokine, formed by them, play the leading role in the formation of immunosuppression under disseminated pulmonary tuberculosis. Besides, subpopulation imbalance of Treg-cells in patients with disseminated pulmonary tuberculosis, in case at multi-drug-resistance and drug-susceptible, is shown by the increased content of T-reg-cells with CD4+CD25+Foxр3+phenotypein combination with the deficiency of CD4+CD25+Foxр3– T-reg lymphocytes in blood. The increase in the amount of CD4+CD25+Foxр3+ T-reg-cells in blood of patients with disseminated multi-drug resistance pulmonary tuberculosis is associated with the increase in basal and BCG-induced production of TGF-bin vitro.

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